Research progress on intra-arterial thrombolysis for the treatment of central retinal artery occlusion / 国际眼科杂志(Guoji Yanke Zazhi)

Central retinal artery occlusion(CRAO), also known as eye stroke, always results in acute and painless visual loss. At present, conservative treatments, such as eye massage, lowering intraocular pressure and vasodilators have little effect on reducing visual loss. Intra-arterial thrombolysis(IAT)has significantly improved prognosis in patients with acute ischemic stroke, thus IAT has been gradually applied in the treatment of CRAO. IAT injects fibrinolytic drugs directly into the ophthalmic artery by a microcatheter, and dissolves the emboli that block the central retinal artery to restore the blood flow of the retina. Theoretically, IAT may be effective for CRAO as what has been found for stroke, but existing clinical studies exhibited inconsistent results. This paper summarizes the feasibility, efficacy, and safety of IAT treatment in CRAO. It will also analyze related factors that affect the prognosis, putting forward potential development directions and providing insights for the further clinical application of IAT.

Discussion on the pathogenesis of "spleen deficiency and dampness accumulation" of colorectal polyps based on intestinal flora and glucose and lipid metabolism / 国际中医中药杂志

Intestinal flora imbalance and abnormal glucose and lipid metabolism are important risk factors and pathological mechanisms of colorectal polyps. "Spleen deficiency and dampness accumulation" is the core pathogenesis of colorectal polyps. The imbalance of intestinal flora is related to spleen deficiency, and the application of Chinese herbs for invigorating spleen is helpful to the recovery of intestinal flora balance. Abnormal glucose and lipid metabolism is related to dampness accumulation, and it is effective to treat it with bitter and spicy herbs or spleen-invigorating and dampness-eliminating herbs. The interaction between intestinal flora imbalance and abnormal glucose and lipid metabolism changes intestinal microenvironment, damages intestinal epithelial cells, causes abnormal proliferation of intestinal stem cells and leads to colorectal polyps, which is consistent with the pathogenesis of spleen deficiency and dampness accumulation in Traditional Chinese Medicine. Thus, we tried to explore the biological connotation of the pathogenesis of "spleen deficiency and dampness accumulation" of colorectal polyps from the perspective of the interaction of intestinal flora and glucose and lipid metabolism, in order to provide reference for identifying high-risk population and analyzing the therapeutic mechanism of compound prescription for invigorating spleen and removing dampness.

Using the Multi-Theory Model (MTM) of Health Behavior Change to Explain Yoga Practice.

BACKGROUND: Diverse groups, including college students, are being encouraged to practice yoga. Research suggests that college students fail to attain the mental and physical benefits of yoga practice. OBJETIVE: The purpose of this study was to utilize the fourth-generation, multi-theory model (MTM) of health behavior change to explain change regarding yoga practice of asanas, shava asana, pranayama, dhyana, yama and niyama among college students. METHOD: This cross-sectional study relied on a quota sample of students 18 years and older attending Jackson State University, a historically black college in Jackson, Mississippi, United States. MEASURES: A 36-item face and content valid instrument was used to collect data. Stepwise multiple regression was used to analyze the survey data for identifying the best possible predictors of yoga practice. A statistical significance level of 0.05 was set a priori. RESULTS: A total of 70 participants, mean age 28.62 years (SD, 6.11), predominately female (84%) and black (87%) completed the survey. The initiation model constructs- changes in the physical environment (ß = 3.99, P = .002) and behavioral confidence (ß = 0.331, P = .008)-were significant, explaining 40% of the variability in the dependent variable. Practice change was statistically significant (F1,65 =7.569; P = .0001; adjusted R2 = 0.460) for the maintenance model, explaining 46% of the variability. CONCLUSION: The MTM model of health behavior change is effective for explaining the intent to initiate and maintain yoga behavior among college students.

Data mining and analysis of the rules of prescriptions in the treatment of epigastric pain in A Hundred Years of Traditional Chinese Medicine Clinicians in China / 国际中医中药杂志

Objective:To analyze the prescriptions for the treatment for epigastric pain in A Hundred Years of Traditional Chinese Medicine Clinicians in China. Methods:The clinical medical records of well-known TCM doctors for the treatment of epigastric pain were included in the A Hundred Years of Traditional Chinese Medicine Clinicians in China. The database of clinical prescriptions and medicines was built, and the ancient and the Ancient and Modern Medical Records Cloud Platform (V 2.1) was applied and the evaluation of drug efficacy, sex, taste, and menstruation were conducted. Descriptive analysis, association rules, hierarchical clustering, and complex network analysis were applied in the rule of medication, drug pairs and core prescriptions. Results:Data was cleaned according to the inclusion and exclusion criteria, and 270 prescriptions were collected, involving 265 kinds of drugs, and the cumulative frequency of drug use was 3 249. The analysis showed that the drugs commonly used by famous traditional Chinese medicine doctors for the treatment of epigastric pain were mainly warm drugs, tast-balance drugs, and mildly cold drugs. The main flavors of medicines were pungent medicines, bitter medicines, sweet medicines, and effects were mainly drugs that regulate qi, clear heat, and invigorate the spleen. The frequency of drug meridians mainly belonged to spleen meridian, stomach meridian, lung meridian, liver meridian, heart meridian. The effects of the drug were mainly to dry dampness, reduce side effects, regulate qi, clear heat, invigorate spleen, and soften liver and analgesics. Association rule analysis found that the commonly used drug pairs were Pinellia- Tangerine Peel, Atractylodes Macrocephala- Poria, Poria- Tangerine Peel, Atractylodes- Tangerine Peel, Amomum tangerine Peel, Ochre- generation- Inula, Rhizoma Coptis- Evodia, and so on. Cluster analysis showed that Liujunzi Decoction, Zuojin Pill, and Shaoyao Gancao Decoction were mainly used. The drug complex network diagram showed that the core prescription of the drug is Xiangsha Liujunzi Decoction. Conclusion:The well-known TCM doctor focuses on strengthening the spleen and regulating qi, reconciling qi and blood, The treatment identified that the mutual warming and clearing, tonifying and deoppilation, and neutral are the main principles.

Expert Consensus on Rheumatic Immune Diseases Responding Specifically to Traditional Chinese Medicine / 中国实验方剂学杂志

In the clinical practice of rheumatic immune diseases in traditional Chinese medicine （TCM），it`s still unclear about the dominant diseases and breakthrough points. It`s urgent missions to formulate TCM diagnosis and treatment guidelines widely recognized and integrated by traditional Chinese medicine and Western medicine. In order to clarify the dominant diseases and breakthrough points in rheumatism，China association of Chinese medicine initiated a research group covering experts in the field of rheumatism of traditional Chinese medicine and Western medicine. Based on questionnaire survey and on-site discussion，experts had reached the following consensus. Evidence-based medicine research using modern medical methods and scientific methods should be carried out to provide objective clinical evidences. "Four mutuality" were put forward as the basis for the work of integrated traditional Chinese and Western medicine，that is the mutual communication using the exchangeable context，the mutual explanation using common theories，the mutual certification using common standards，and the mutual integration using common means. Key works should focus on solving refractory rheumatism in the future. In terms of dominant diseases and breakthrough points，this paper introduces 21 breakthrough points in 6 dominant diseases，including rheumatoid arthritis，ankylosing spondylitis，Sjogren's syndrome，hyperuricemia and gout，systemic lupus erythematosus and fibromyalgia syndrome. Advice on this discussion can provide valuable references for developing the treatment scheme of rheumatism with TCM and integrated Chinese and Western medicine and clinical practice and scientific research.

Rauwolfia vomitoria extract suppresses benign prostatic hyperplasia by reducing expression of androgen receptor and 5α-reductase in a rat model / 中西医结合学报

OBJECTIVE@#Herbal medicine is an important therapeutic option for benign prostatic hyperplasia (BPH), a common disease in older men that can seriously affect their quality of life. Currently, it is crucial to develop agents with strong efficacy and few side effects. Herein we investigated the effects of the extract of Rauwolfia vomitoria, a shrub grown in West Africa, on BPH.@*METHODS@#Rats with testosterone-induced BPH were treated with R. vomitoria. Prostates were histologically analyzed by Hematoxylin and eosin staining. Proliferation index and the expression levels of androgen receptor and its associated proteins were quantified through immunohistochemistry and immunoblotting. Androgen receptor target genes were examined by quantitative real-time polymerase chain reaction. The sperm count and body weight of rats were also measured.@*RESULTS@#The oral administration of R. vomitoria extract significantly reduced the prostate weight and prostate weight index in BPH rats, supported by the decreased thickness of the prostate epithelial layer and increased lumen size. Similar effects were observed in the BPH rats treated with the reference drug, finasteride. R. vomitoria extract significantly reduced the testosterone-induced proliferation markers, including proliferating cell nuclear antigen and cyclin D1, in the prostate glands of BPH rats; it also reduced levels of androgen receptor, its associated protein steroid 5α-reductase 1 and its downstream target genes (FK506-binding protein 5 and matrix metalloproteinase 2). Notably, compared with the finasteride group, R. vomitoria extract did not significantly reduce sperm count.@*CONCLUSION@#R. vomitoria suppresses testosterone-induced BPH development. Due to its milder side effects, R. vomitoria could be a promising therapeutic agent for BPH.

Advances in Research on Different Compatibility Ratios of Coptidis Rhizoma-Evodia Fructus / 中国实验方剂学杂志

Coptidis Rhizoma-Evodia Fructus is a classic herb pair in traditional Chinese medicine prescriptions, the famous prescription is called Zuojinwan, which comes from Danxi Xinfa, is composed of Coptidis Rhizoma-Evodia Fructus (6∶1). In this formula, Coptidis Rhizoma has the effect of clearing heat and drying diuresis, purging fire to remove toxin and clearing heart. Evodia Fructus has the effects of expelling cold and alleviating pain, checking upward adverse flow of Qi tostop vomiting, and assisting yang to stop diarrhea. Coptidis Rhizoma has the properties of bitter and cold, and Evodia Fructus has the properties of pungent and calidus. Pungent drugs have divergent effects, and bitter drugs have sedimentation effect, when used in combination, they can clear the liver and purge fire, calm the adverse-rising energy and stop vomiting. On the basis of Zuojinwan, Coptidis Rhizoma-Evodia Fructus medicine has derived different compatibility ratios. Different ratios are different in terms of efficacy, usage, clinical application. Although with the application of modern analytical instruments and the development of molecular pharmacology theory, the chemical constituents and Pharmacological effects of Coptidis Rhizoma and Evodia Fructus have been fully studied, as to the principle of compatibility, and the study of pharmacological effects and chemical constituents after the compatibility of the two drugs in different proportions, there is still no comprehensive system summary. This article makes a systematic and comprehensive explanation of Coptidis Rhizoma-Evodia Fructus from the aspects of famous literature, chemical composition, pharmacological effects and clinical applicationthrough querying literature and ancient books. In order to make this herb pair more standardized, and provide reference materials for further research and development for this herb pair.

The role of traditional Chinese medicine characteristic lung rehabilitation for treatment of patients with chronic obstructive pulmonary disease and syndrome of lung and kidney qi deficiency at steady state / 中国中西医结合急救杂志

Objective To observe the clinical effect of traditional Chinese medicine (TCM) characteristic lung rehabilitation in treatment of patients with chronic obstructive pulmonary disease (COPD) and TCM syndrome of lung and kidney qi deficiency at stable period. Methods Sixty patients with stable COPD and lung and kidney qi deficiency syndrome admitted to the First Affiliated Hospital of Anhui University of Chinese Medicine from June to August 2017 were enrolled, and they were divided into routine treatment group and lung rehabilitation treatment group according to the random number table method, each group 30 cases. The routine treatment group was given Seretide (serevent/futicasone) dry powderi nhalation therapy; on the basis of therapy in the routine treatment group, the lung rehabilitation treatment group was treated with TCM characteristic lung rehabilitation technology (acupoint application + Chinese medicine ionic induction + oral administration of Chinese medicine Liuweibuqi granules, delivery at appropriate intervals); both groups were treated for 2 months. The changes of TCM syndrome score, western medicine symptom score, the times of acute exacerbation of COPD, COPD assessment test (CAT) score, lung function indexes: forced expiratory volume in one second (FEV1), FEV1/forced vital capacity (FVC) were observed before and after treatment in two groups. Results After treatment, TCM syndrome score, western medicine symptom score, CAT score, and after treatment the times of acute exacerbation of COPD in both groups were significantly lower than those before treatment, and the above indexes in the lung rehabilitation treatment group were markedly lower than those in routine treatment group [TCM syndrome score:11.93±1.80 vs. 14.27±2.88, western medicine symptom score: 14.20±2.75 vs. 11.93±4.23, CAT score: 14.87±2.60 vs. 16.23±4.39, the times of acute exacerbation of COPD (times): 0.63±0.49 vs. 0.95±0.83, all P < 0.05]. The improvement of FEV1 in the two groups was not significant; but FEV1/FVC in lung rehabilitation treatment group was obviously higher than that before treatment, FEV1/FVC in lung rehabilitation treatment group was significantly higher than that in the routine treatment group [(57.93±7.27)% vs. (52.49±6.61)%, P < 0.05]. Conclusion The application of TCM characteristic lung rehabilitation in the treatment of COPD patients with stable lung and kidney qi deficiency syndrome based on bronchodilators and glucocorticoids can reduce the number of acute exacerbation, improve the patients' clinical symptoms and living quality, but the improvement of lung function is not significant.

Comparison of MRI features and pathological findings between intracranial solitary fibrous tumors and hemangiopericytomas / 实用放射学杂志

Objective To analyze MRI features of intracranial solitary fibrous tumors (SFT)and hemangiopericytomas (HPC)and combined with pathology to improve recognition of the diseases.Methods MRI and pathologic data of 13 intercranial SFT and 9 HPC were analyzed retrospectively.Results The intracranial SFT were round or oval,well-defined,with internal hypointensity and obviously enhanced area on T2and enhanced MRI-so called"yin-yang"pattern,rare hemorrhage,slight peritumoral edema and rare skull destruction.The HPC were lobulated or irregular,unclear margin,hyperintensity on T2,common hemorrhage,obvious peritumoral edema and common skull destruction.The differences between the two tumors were significant in relation to the tumor shape,T2signal,"yin-yang"pattern, hemorrhage and skull destruction(P<0.05).There were no significant differences in relation to the internal cystic necrosis,peritumoral edema, dural attachment and dural tail sign (P>0.05).Both of them showed the similar immunohistochemical features:the expression of Vim,CD34,CD99 and Bcl-2 were positive,and the expression of EMA was negative.Conclusion The MRI features of intracranial SFT and HPC are different in tumor shape,T2signal,"yin-yang"pattern,hemorrhage and skull destruction.MRI features are helpful to diagnosis and judge prognoses preliminary.

Clinical observation of Sweet Dream Oral Liquid curing deficiency of spleen and kidney style MCI / 中草药

Objective To observe the clinical effect of Sweet Dream Oral Liquid on the mild cognitive impairment (MCI) patients of spleen and kidney deficiency. Methods A total of 102 patients with MCI were randomly divided into two groups, the treatment group with 53 patients and the control group with 49 patients. Both groups were given Citicoline Sodium Tablets for 8 weeks. The treatment group was given Sweet Dream Oral Liquid extra. Mini-mental state examination (MMSE), Transcranial Doppler (TCD) and Traditional Chinese Medicine Syndrome Score (TCMSS) were performed before and after the treatment. Compliance and adverse reactions for both groups were observed during the treatment. Results MMSE improved after the treatment for both groups; Especially, MMSE of the treatment group improved more obviously (P < 0.01); The difference between the two groups was significant (P < 0.05); For treatment group, the average blood flow velocity (Vm) of the cerebral arteries was increased significantly after the treatment(P < 0.01); Compared with the control group, the difference was obvious (P < 0.05); The total effective rate of the treatment group on spleen and kidney deficiency syndrome was 96.23%. It was 18.37% for the control group. Difference between the two groups was significant (P < 0.01). After the treatment, TCMSS of the treatment group was significantly reduced (P < 0.01); It was a significant difference comparing with the control group (P < 0.01). During the treatment, both groups had a good compliance, no adverse reactions and no shedding patients. Conclusion Sweet Dream Oral Liquid can improve the cognitive function and living quality of the patients with deficiency of spleen and kidney style MCI by reducing TCMSS, improving cerebral hemodynamics and rising MMSE score.

Laser Scanning Confocal Microscopical Study of the Effect of Acupuncture and Medicine on NSC Differentiation in Rats with Stroke of qi Deficiency and Blood Stasis Type / 上海针灸杂志

Objective To investigate the effect of cluster needling at scalp points plus gavage of modified Buyang Huanwu decoction on NSC differentiation in rats with ischemic stroke of qi deficiency and blood stasis type.Methods A model of qi deficiency and blood stasis syndrome was made using healthy rats. Dil dye was given for pre-labelling after the success of model making. The rats were randomized into groups A, B, C, D, E and F. Group F consisted of 4 rats and each of the other groups, of 12 rats. A rat model of ischemic stroke of qi deficiency and blood stasis type was made by MCAO at 48 hrs after pre-labelling. After the success of model making, group A was the model group, group B was treated by cluster needling at scalp points, group C was treated by an oral gavage of Buyang Huanwu decoction, group D was treated by an oral gavage of modified Buyang Huanwu decoction, group E was treated by cluster needling at scalp points plus an oral gavage of modified Buyang Huanwu decoction and group F was the sham operation group. The rats were sacrificed at various time points respectively and the materials were taken. By nerve function assessment, double immunofluorescence staining and laser scanning confocal microscopy, a comparative study was conducted to investigate the effects of different treatments on NSC differentiation.Results There was a statistically significant difference in the nerve function score between group B, C, D, E or F and group A after one week of treatment (P<0.01), between group B or E and group A after two weeks of treatment (P<0.05,P<0.01) and between group E and group C or D after one and two weeks of treatment (P<0.01,P<0.05). After one and two weeks of treatment, there were statistically significant differences in Brdu﹢/NeuN﹢ cell count, Brdu﹢/GFAP﹢ cell count, Dil﹢/Brdu﹢NeuN﹢ cell count and Dil﹢/Brdu﹢/GFAP﹢ cell count between group B, C, D or E and group A (P<0.01), between group D or E and group B or C (P<0.01) and between groups E and D (P<0.01).Conclusions Various treatments have a promoting effect on nerve stem cell differentiation in the rats. Of various treatments, cluster needling at scalp points plus gavage of modified Buyang Huanwu decoction has the best therapeutic effect.

The Usage and Economic Analysis in Using Traditional Chinese Medicine Injection in a Hospital from 2011 to 2013 / 中国卫生经济

To analyze clinical usage of traditional Chinese medicine ( TCM ) injections in a hospital , to evaluate the rationality and economy, so as to promote drug rational use. Methods: Based on the quantity of issue drugs in computer information management system, using the amount of sorting method and the dosage frequency analysis method, according the defined daily doses (DDDs) of the drug to calculate DDDs and the average daily cost(DDC) , sort the total amount of drug usage and DDDs then seek their ratio. Results:Medicine of the cardiovascular system and the antitumor system is rising in a hospital from 2011 to 2013. Amount structure of cardiovascular system drugs is always beyond 60%, and total usage amount of TCM injections remained as top 3. Individual TCM injections sort ratio exceeds the specified range. Conclusion:In overall, the clinical TCM injections use synchronism better, which is basic rational in a hospital, the clinical departments could choose the drug of appropriate price according to actual condition.

[Study on the quality and genetic diversity of Gentiana macrophylla pall of different habitats].

OBJECTIVE: To study the quality and genetic diversity in Gentiana macrophylla of different habitats for controlling the quality of Gentiana macrophylla. METHODS: Main characteristics and microscopic identification were adapted to identify Gentiana macrophylla; HPLC method was applied for analysising the contents of Gentiana macrophylla. RAPD method was applied to study genetic structure and genetic diversity of Gentiana macrophylla. RESULTS: The experiment showed that the Gentiana macrophylla of different habitats have different main charactericstics, microscopic identifications and contents, and effective ingredient contents of gentiopicroside. The levels of population genetic diversity of various groups followed by HUANGZHONG County population (K) > HUAN County population (J) > ZiWU Mountain population (H). CONCLUSION: The study of external morphology, internal structure, active ingredient content and genetic level of Gentiana macrophylla from different areas provides a scientific basis for protecting wild species and ensuring the quality in introducing and cultivating Gentiana macrophylla.

Isolation and identification of chemical constituents from Taxus cuspidta Sibe et Zucc / 药学学报

Three compounds were isolated from the extract of Taxus cuspidta Sibe et Zucc with the column chromatography on silica gel and preparative HPLC methods. Their structures were identified according to the physicochemical properties and spectral analysis, and they were identified as (E)-1-methoxy-2-O-(p-coumaroyl)-myo-inositol (1), 2-deacetoxy-7beta, 9a, 10beta-trideacetyltaxinine J (2) and (3aS, 4aR, 6S, 8S, 8aS, 9R, 10R, 10aS)-benz[f]azulene-6, 8, 9, 10 (3H)-terol, 3a, 4, 4a, 5, 6, 7, 8, 8a, 9, 10-decahydro-10a-(1-hydroxyl-1-methylethyl)-1, 8a-dimethyl-5-methylene (3). Among them, compound 1 was a new compound, and compounds 2, 3 were two novel natural products.

Flavonoids and phenolic acid derivatives from flos farfarae / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the chemical constituents of the flower buds of Tussilago farfara.</p><p><b>METHOD</b>The chemical constituents were isolated by silica gel and Sephadex LH-20 column chromatography, and structurally elucidated by spectral evidence together with physiochemical properties.</p><p><b>RESULT</b>Seven flavonoids, quercetin (1), quercetin-3-O-beta-D-glucopyranoside (2), quercetin-4'-O-beta-D-glucopyranoside (3), hyperoside (4), rutin (5), kaempferol (6), kaempferol-3-O-alpha-L-rhamnopyranosyl-(1-6)-beta-D-glucopyranoside (7), together with eight phenolic acid derivatives, caffeic acid (8), methyl caffeate (9), ethyl caffeate (10), (E)-2,5-dihydroxycinnamic acid (11), 3,4-di-O-caffeoylquinic acid (12), 4,5-di-O-caffeoylquinic acid (13), methyl4,5-di-O-caffeoylquinate (14) and chlorogenic acid (15) were isolated from the flower buds of Tussilago farfara.</p><p><b>CONCLUSION</b>Compounds 7, 9-14 are isolated from this plant, also from this genus, for the first time.</p>

[Preparation technology of Zhangyanming dispersible tablet and determination of puerarin by HPLC].

OBJECTIVE: To study the extraction and pharmceutics technology of Zhangyanming dispersible tablet and establish the method for determining the content of puerarin by HPLC. METHODS: Using total extracted puerarin and disintegrating time as evaluation indexes and the conditions for determination were as follows: Hypersil BDS C18 column (5 microm, 4.6 mm x 200 mm), Methanol-H2O (25:75) as the mobile phase, the detection wavelength at 250 nm, the column temperature at 30 degrees C, and 1.0 mL/min as the mobile phase speed. RESULTS: The calibration curve was linear at the range of 0.1699 to 1.3594 microg for the puerarin content and the linear equation was y = 3.054 x 10(6)x - 24019 (r = 0.9998). The average recovery ratio was 100.03%, and the relative standard deviation was 1.79%. CONCLUSIONS: The new preparation technology is reasonable and HPLC can be used to determine the content of puerarin in Zhangyanming dispersible tablet because the operation was convenient, fast, easy to repeat, and the analysis results were accurate and especial.

[Study on discrimination of tea based on color of multispectral image].

Tea is one of the most popular beverages worldwide. Its categories have a great relationship to its beneficial medicinal properties. The present work attempted to study the feasibility to use multispectral imaging technique as a rapid and non-destructive method to discriminate tea varieties. Two categories of tea discriminated hardly by naked eye were sorted. The images were 1 036 pixels vertically by 1 384 pixels horizontally with 24-bit depth, and were captured using a red (R) waveband, near infrared (NIR) waveband and green (G) waveband multispectral digital imager, MS3100 (Duncan Technologies, Inc., CA, USA). The three wavebands of image (Red, Green, NIR) can be composed into one image which contains more information than images recorded by ordinary digital cameras, especially, the NIR image is more sensitive to the color of organic matter than visible spectrum. The three images of one sample can be obtained simultaneously. The color features of tea were calculated using the standard notations: mean and mean square deviation. Then, the two color features of 3CCD and ordinary digital cameras were extracted and calculated by Matlab 7. 3 software respectively, and were contrasted. A total of 60 samples were adopted, and the features of mean and mean square deviation of NIR waveband image were applied as inputs to a back propagation neural network (BP-ANN) with one hidden layer. The forty samples (twenty for each category) were selected randomly to build BP-ANN model, and this model was used to predict the varieties of 20 unknown samples (ten for each category). The two categories of tea can be discriminated by the information of color of images of 3CCD, but can not by the ordinary digital cameras. The result indicted that the discrimination rate of classification set of BP-ANN model was up to 100% within 0.3 of threshold. It concluded that multi-spectral imaging technique has a high potential to identify categories of green tea fast and non-destructively.

Effects of SDZ on LOVO Cell Line and the Expression of E-Cadherin and N-Cadherin / 中国药房

OBJECTIVE:To investigate the effects of SDZ on LOVO cell line and the expression of E-Cadherin and N-Cadherin. METHODS: The apoptosis of SDZ on growth of LOVO cells was observed under electron microscope and inverted microscope. The expression of E-Cadherin and N-Cadherin treated by SDZ were detected by RT-PCR. RESULTS: SDZ promoted the apoptosis of LOVO cell lines and increased the expression of E-Cadherin,however,it did no effect on the expression of N-Cadherin. CONCLUSION: SDZ can enhance the intercellular adhesiveness and degrade the capability of infiltration and metastasis of tumor cells.

Scanning of drug targets related to uterus contraction from the uterine smooth muscles by cDNA microarray / 中南大学学报(医学版)

OBJECTIVE@#To screen the differentially expressed gene profile from the smooth muscles in the fundus uterus at the active stage of labor, and to provide candidate genes for picking out the drug targets related to uterine contraction.@*METHODS@#Differentially expressed genes of uterine smooth muscles in the corpus from pro and post spontaneous parturition and those induced by oxytocin,as well as those from the corpus and the lower portion spontaneous parturition,were scanned respectively by human full-length genetic cDNA microarray with 8064 probe sets. Semi-quantitative RT-PCR was applied to testify the expression of voltage dependent calcium channel-L subtype (CACNA). The differentially expressed genes in the structure and function of the drug targets were picked out by bio-informatics to serve as candidate drug targets related to uterine contraction.@*RESULTS@#The expressions of 29 genes were upregulated in fundus smooth muscles from the pro and post natural parturition, the pro and post inductive parturition of oxytocin, and the natural parturition. The expression of CACNA gene in RT-PCR was in accordance with that in the microarray. Among the 29 genes, neuromedin B receptor (NMBR) gene and neuropeptide Y (NPY) gene were the genes which not only had the targets of uterine contracted medicine, but also could contract the uterine. The differential expression ratios of NMBR in the above 3 types of uterine myometrium were 6.9,11.3, and 9.0, respectively while those of NPY were 6.0,29.8, and 2.9 respectively.@*CONCLUSION@#NMBR, whose expression in the uterine smooth muscles is always up-regulated at different parturition conditions, is likely to be an ideal candidate target of uterotonic drugs.

Clinical and experimental study of xiaoshui decoction in the treatment of primary liver cancer caused ascites / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To observe the clinical efficacy of Xiaoshui Decoction (XSD) in treating ascites in patients suffered from primary liver cancer of Pi-deficiency with damp harassment syndrome (PDDHS) as well as to study the effect through the experiment in mice.</p><p><b>METHODS</b>Sixty-one patients confirmed to be primary liver cancer of PDDHS and accompanied with ascites were randomly divided into the treated group (n=33) and the control group (n=28). The treated group was treated by XSD combined with chemotherapy by locally applying of DDP via abdominal infusion, while the control group treated by DDP infusion alone. The treatment lasted for two months. The conditions of ascites, quality of life (QOL), survival period, and TCM syndrome after treatment were observed. In the experimental study, the mice models of ascites were grouped and treated to observe the conditions of ascites and their survival period.</p><p><b>RESULTS</b>The short-term total effective rate of the treated group and the control group was 42.4% and 21.4%, the interval of aspirating ascites after treatment was 17.95 +/- 9.63 days and 10.87 +/- 7.76 days, and the 1-year survival rate 33.3% and 14.3%, respectively, significant difference was shown between the two groups in the three parameters (all P < 0.05 ). QOL was improved in both groups with insignificant difference (P > 0.05). Besides, the main symptoms were improved in patients of both groups, especially in the ameliorating of fatigue, abdominal distension, nausea and vomiting. Evaluation on safety of treatment showed that XSD had no obvious toxic and adverse reaction, and so it was safe in use. Experimental study showed that on the two mice models of ascites induced by inoculating two kinds of tumor cell, the effect of XSD was superior to that of the control group in aspects of reducing ascites and prolonging survival period, showing significant difference (P < 0.05).</p><p><b>CONCLUSION</b>Satisfactory short-term efficacy in treating primary liver cancer with ascites of the Pi-deficiency with damp harassment syndrome could be obtained by XSD. Its effect in prolonging survival period was confirmed by experimental study. XSD can also improve the symptoms and QOL of patients, therefore, it is an effective and reliable remedy for treatment of primary liver cancer with ascites.</p>