RESUMO
Objective: To investigate the clinicopathologic features and possible causes of granulomatous lobular mastitis(GLM). Methods: Three hundred cases of GLM were collected from surgical specimens diagnosed at Longhua Hospital, Shanghai University of Traditional Chinese Medicine from January 2015 to November 2017. Morphologic features were reviewed using HE staining. A total of 116 cases were investigated by Gram staining. The expression of CD3, CD20, CD68, IgG, IgG4, CD38 and CD138 was detected by immunohistochemical staining. Results: The age of the patients was 23 to 47 years and the median age was 32 years. All patients were female, 96.7% (290/300) had a history of lactation.There were 143 cases of left breasts, 138 cases of right breast and 19 cases of bilateral breasts. Serum prolactin increased in 39.7%(119/300) patients. Within 15.7%(47/300) of patients were associated with nodular erythema or joint swelling and pain of the lower extremities. Pathological observation showed that lobular-centric suppurative granulomatous inflammation, accompanied by dilatation of intralobular and interlobular ducts. There were 16 cases accompanied with duct ectasia. Immunohistochemistry showed CD3-positive lymphocytes were more than CD20-positive lymphocytes in the peripheral aggregation zone of neutrophils within granulomatous lesions. Gram positive bacteria were found in the lipid vacuoles of the 51.7%(60/116) patients. Conclusions: GLM has distinctive histologic features. It may be related to corynebacterium infection, or accompanied by the increase of serum prolactin and erythrocyte sedimentation rate. The age, location and history of the disease are importance in the diagnosis and differential diagnosis.
Assuntos
Mama/patologia , Mastite Granulomatosa/patologia , Adulto , Mama/microbiologia , Aleitamento Materno , China , Feminino , Mastite Granulomatosa/sangue , Mastite Granulomatosa/diagnóstico , Mastite Granulomatosa/microbiologia , Humanos , Pessoa de Meia-Idade , Adulto JovemRESUMO
Two new fatty abietane diterpenoids, oleoyl neocryptotanshinone (1) and oleoyl danshenxinkun A (2), were isolated as minor components from the roots of Salvia miltiorrhiza. Their structures were established on the basis of spectral evidence. In preliminary tests, they selectively inhibited rabbit platelet aggregation induced by arachidonic acid.
Assuntos
Diterpenos/química , Ácidos Oleicos/química , Plantas Medicinais/química , Inibidores da Agregação Plaquetária/química , Animais , China , Diterpenos/isolamento & purificação , Diterpenos/farmacologia , Hidrólise , Técnicas In Vitro , Espectroscopia de Ressonância Magnética , Ácidos Oleicos/isolamento & purificação , Ácidos Oleicos/farmacologia , Raízes de Plantas/química , Agregação Plaquetária/efeitos dos fármacos , Inibidores da Agregação Plaquetária/isolamento & purificação , Inibidores da Agregação Plaquetária/farmacologia , Coelhos , Espectrofotometria UltravioletaRESUMO
Two minor acetophenones, 2,5-dihydroxy-4-methoxy-acetophenone (2) and 2,5-dihydroxy-4-methylacetophenone (7) from Paeonia species were found to selectively inhibit the aggregation of rabbit platelets induced by arachidonic acid. They were more potent than the major compound, paeonol (1), and 7 also inhibited the formation of TXA2 and PGD2 from arachidonic acid.
Assuntos
Acetofenonas/farmacologia , Plantas Medicinais/química , Inibidores da Agregação Plaquetária/farmacologia , Animais , Ácido Araquidônico/farmacologia , Plaquetas/efeitos dos fármacos , Plaquetas/fisiologia , Técnicas In Vitro , Raízes de Plantas/química , CoelhosRESUMO
A new hexacyclic triterpenoid, mudanpinoic acid A (1), and a new gallic acid glycoside, mudanoside B (2), along with nine known compounds--benzoic acid, resacetophenone, paeoniflorigenone, beta-sitosterol, betulinic acid, oleanoic acid, quercetin, beta-sitosterol-beta-D-glucoside, and trans-caffeic acid stearyl ester-were isolated from the dried root cortex of Paeonia suffruticosa. The structures of the novel compounds were elucidated on the basis of spectral methods, and that of compound 1 was confirmed by X-ray crystallographic analysis.