RESUMO
To study chemical constituents contained in Tetraena mongolica. Chemical constituents were separated and purified by using such methods as silica gel, Toyopearl HW-40C and HPLC preparative chromatography. Their structures were identified by organic spectral method. One new compound was separated from T. mongolica and identified olean-11-oxo-12-en-28-ol-3beta-yl-caffeate.
Assuntos
Compostos Orgânicos/análise , Zygophyllaceae/química , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/normas , Compostos Orgânicos/química , Compostos Orgânicos/isolamento & purificação , Controle de QualidadeRESUMO
A chemical investigation of the endemic relict shrub Tetraena mongolica led to the isolation of four new triterpenes: 11α,12α:13ß,28-diepoxyoleanane-3ß-yl trans-caffeate (1), 3ß-hydroxy-11α,12α-epoxyoleanane-28-al (2), olean-11-en-28-al-3ß-yl trans-caffeate (3), and 28-acetoxy-olean-12-en-3ß-yl trans-caffeate (4). Their structures were elucidated by extensive spectroscopic methods.
Assuntos
Medicamentos de Ervas Chinesas/isolamento & purificação , Saponinas/isolamento & purificação , Triterpenos/isolamento & purificação , Zygophyllaceae/química , Medicamentos de Ervas Chinesas/química , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Saponinas/química , Triterpenos/químicaRESUMO
OBJECTIVE: To study the immunosuppressive constituents from Tetraena mongolica. METHOD: Chemical constituents were isolated and purified by repeated column chromatography( silica gel, Toyopearl HW40C and preparative HPLC). Their structures were elucidated on the basis of spectral data analysis. The MTT assay was applied to evaluate the isolated compounds on the inhibition effect of lymphocyte transformation. RESULT: Six triterpenes were isolated and their structures were identified as follows: 3beta-hydroxy-11alpha, 12alpha:13beta,28-diepoxyoleanane(1), 3beta-(3, 4-dihydroxycinnamoyl)-erythrodi-ol(2), olean-28-al-3beta-yl-caffeate(3), erythrodiol (4), 12-oleanaen-3beta-caffeate(5), 3-O-(E) -coumaroylerythrodiol(6). Compound 24 exhibited the inhibition effects on lymphocyte transformation. CONCLUSION: Compounds 1-6 were isolated from this plant for the first time, and compound 1 was a new nature product. Compound 2-4 showed significant immunosuppressive activity.
Assuntos
Imunossupressores/química , Imunossupressores/isolamento & purificação , Triterpenos/química , Triterpenos/isolamento & purificação , Zygophyllaceae/química , Animais , Células Cultivadas , Imunossupressores/farmacologia , Ativação Linfocitária/efeitos dos fármacos , Espectroscopia de Ressonância Magnética , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Triterpenos/farmacologiaRESUMO
A family of unusual proteins is deposited in flat, structural platelets in reflective tissues of the squid Euprymna scolopes. These proteins, which we have named reflectins, are encoded by at least six genes in three subfamilies and have no reported homologs outside of squids. Reflectins possess five repeating domains, which are highly conserved among members of the family. The proteins have a very unusual composition, with four relatively rare residues (tyrosine, methionine, arginine, and tryptophan) comprising approximately 57% of a reflectin, and several common residues (alanine, isoleucine, leucine, and lysine) occurring in none of the family members. These protein-based reflectors in squids provide a marked example of nanofabrication in animal systems.