RESUMO
The objective of this study was to determine the optimum conditions for calcium oxide (CaO) treatment of anaerobically stored corn stover by in situ and in vitro methods. Four ruminally cannulated, non-lactating, non-pregnant Holstein cows were used to determine the in situ effective degradabilities of dry matter (ISDMD), organic matter (ISOMD), neutral detergent fibre (ISNDFD), in vitro organic matter disappearance (IVOMD) and gas production in 72 h (GP72h ) of corn stover. A completely randomized design involving a 3 × 3 factorial arrangement was adopted. Ground corn stover was treated with different levels of CaO (3%, 5% and 7% of dry stover) at varying moisture contents (40%, 50% and 60%) and stored under anaerobic conditions for 15 days before analysis. Compared with untreated corn stover, the CaO-treated stover had increased ash and calcium (Ca) contents but decreased aNDF and OM contents. The moisture content, CaO level and their interaction affected (p < 0.01) the content of aNDF, ash and OM, and the ratio of aNDF/OM. The greatest ISDMD, ISOMD and ISNDFD were observed when stover was treated with 7% CaO and 60% moisture, while no differences (p > 0.01) in these in situ degradability parameters were observed between the stover treated with 5% CaO at 60% moisture content and those treated with 7% CaO at 60% moisture content. Corn stover treated with 5% CaO at 50% moisture had the maximum IVOMD and GP72 h among the treatments, and there was no difference (p > 0.01) between 50% and 60% moisture. Results from this study suggested that 5% CaO applied at 60% moisture could be an effective and economical treatment combination.
Assuntos
Ração Animal/análise , Compostos de Cálcio/química , Bovinos/fisiologia , Óxidos/química , Água/química , Zea mays , Anaerobiose , Animais , Digestão/fisiologia , Feminino , Armazenamento de Alimentos , Concentração de Íons de Hidrogênio , MetanoRESUMO
Nutritional status and glucose precursors are known regulators of gluconeogenic gene expression. Glycerol can replace corn in diets fed to dairy cows and use of glycerol is linked to increased rumen propionate production. The effect of dietary glycerol on the regulation of gluconeogenic enzymes is unknown. The objective of this study was to examine the effect of glycerol on expression of pyruvate carboxylase (PC), cytosolic and mitochondrial phosphoenolpyruvate carboxykinase (PEPCK-C and PEPCK-M), and glucose-6-phosphatase. Twenty-six multiparous Holstein cows were fed either a control diet or a diet where high-moisture corn was replaced by glycerol from -28 through +56 d relative to calving (DRTC). Liver tissue was collected via percutaneous liver biopsy at -28, -14, +1, +14, +28, and +56 DRTC for RNA analysis. Expression of PC mRNA increased 6-fold at +1 and 4-fold at +14 DRTC relative to precalving levels. Dietary glycerol did not alter expression of PC mRNA expression. Expression of PEPCK-C increased 2.5-fold at +14 and 3-fold at +28 DRTC compared with +1 DRTC. Overall, dietary glycerol increased PEPCK-C expression compared with that of cows fed control diets. The ratio of PC to PEPCK-C was increased 6.3-fold at +1 DRTC compared with precalving and tended to be decreased in cows fed glycerol. We detected no effect of diet or DRTC on PEPCK-M or glucose-6-phosphatase mRNA, and there were no interactions of dietary treatment and DRTC for any transcript measured. Substituting corn with glycerol increased the expression of PEPCK-C mRNA during transition to lactation and suggests that dietary energy source alters hepatic expression. The observed increase in PEPCK-C expression with glycerol feeding may indicate regulation of hepatic gene expression by changes in rumen propionate production.
Assuntos
Glicerol/administração & dosagem , Fígado/enzimologia , Ração Animal/análise , Animais , Bovinos , Óleo de Sementes de Algodão , Dieta/veterinária , Feminino , Regulação da Expressão Gênica , Gluconeogênese , Glucose-6-Fosfatase/genética , Glucose-6-Fosfatase/metabolismo , Medicago sativa , Micronutrientes/administração & dosagem , Micronutrientes/análise , Fosfoenolpiruvato Carboxiquinase (ATP)/genética , Fosfoenolpiruvato Carboxiquinase (ATP)/metabolismo , Piruvato Carboxilase/genética , Piruvato Carboxilase/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Rúmen/metabolismo , Zea maysRESUMO
Growth of the corn ethanol industry has created a need for alternatives to corn for lactating dairy cows. Concurrent expansion in soydiesel production is expected to increase availability and promote favorable pricing for glycerol, a primary co-product material. The objective of this study was to determine the feeding value of glycerol as a replacement for corn in diets fed to lactating dairy cattle. Sixty lactating Holstein cows housed in individual tie stalls were fed a base diet consisting of corn silage, legume forages, corn grain, soyhulls, roasted soybeans, and protein supplements. After a 2-wk acclimation period, cows were fed diets containing 0, 5, 10, or 15% refined glycerol for 56 d. Cows were milked twice daily and weekly milk samples were collected. Milk production was 36.3, 37.2, 37.9, and 36.2 +/- 1.6 kg/d and feed intake was 23.8, 24.6, 24.8, and 24.0 +/- 0.7 kg/d for 0, 5, 10, and 15% glycerol treatments, respectively, and did not differ except for a modest reduction in feed intake during the first 7 d of the trial for 15% glycerol (treatment x time effect). Milk composition was not altered by glycerol feeding except that milk urea nitrogen was decreased from 12.5 +/- 0.4 to 10.2 +/- 0.4 mg/dL with glycerol addition. Cows fed diets containing 10 and 15% glycerol gained more weight than those fed rations containing 0 or 5% glycerol but body condition scores did not differ with glycerol feeding. The data indicate that glycerol is a suitable replacement for corn grain in diets for lactating dairy cattle and that it may be included in rations to a level of at least 15% of dry matter without adverse effects on milk production or milk composition.