Response of garlic (Allium sativum L.) to the combined toxicity of microplastics and arsenic.

The extensive utilization of mulch films in agricultural settings, coupled with the persistence of microplastic remnants in soil following the natural degradation of plastics, has given rise to detrimental microplastic impacts on crops. Arsenic (As) contamination in the environment is known to accumulate in crops through aquatic pathways or soil. Garlic (Allium sativum L.), a globally popular crop and seasoning, contains alliin, a precursor of its flavor compounds with medicinal properties. While alliin exhibits antimicrobial and antioxidant effects in garlic, its response to microplastics and arsenic has not been thoroughly investigated, specifically in terms of microplastic or As uptake. This study aimed to explore the impact of varied stress concentrations of microplastics on the toxicity, migration, and accumulation of As compounds. Results demonstrated that polystyrene (PS) fluorescent microspheres, with an 80 nm diameter, could permeate garlic bulbs through the root system, accumulating within vascular tissues and intercellular layers. Low concentrations of PS (10 and 20 mg L-1) and As (2 mg L-1) mitigated the production and accumulation of reactive oxygen species (ROS) and antioxidant enzymes in garlic. Conversely, garlic exhibited reduced root vigor, substance uptake, and translocation when treated with elevated As concentrations (4 mg L-1) in conjunction with PS concentrations of 40 and 80 mg L-1. An escalation in PS concentration facilitated As transport into bulbs but led to diminished As accumulation and biomass in the root system. Notably, heightened stress levels weakened garlic's antioxidant defense system, encompassing sulfur allicin and phytochelatin metabolism, crucial for combating the phytotoxicity of PS and As. In summary, PS exerted a detrimental influence on garlic, exacerbating As toxicity. The findings from this study offer insights for subsequent investigations involving Liliaceae plants.

Effects of foliar application of graphene oxide on cadmium uptake by lettuce.

Although graphene oxide (GO) has been widely used to enhance soil quality and crop yield, there is currently little information regarding the effects of foliar application of GO on cadmium (Cd) toxicity to plants. In this study, we investigated the response to GO in lettuce cultivated under Cd stress in hydroponic conditions. Lettuce was grown from seeds in a nutrient solution supplemented with 2 mg/L Cd and the leaves were sprayed with 0, 30, and 60 mg/L GO. The results indicated that application of 30 mg/L GO significantly increased the total length, surface area, average diameter, and hair number of lettuce roots, and effectively alleviated the negative effects of Cd on root growth. Furthermore, foliar application of 30 mg/L GO, but not 60 mg/L GO, significantly improved the quality of lettuce, including reduction in Cd accumulation in leaves and roots and increase in soluble sugar, protein, and vitamin C content. Transmission electron microscopy revealed that GO nanoparticles, which entered the leaves and were subsequently transported to the roots via the vascular system (phloem), reduced the damaging effect of Cd on cellular organelles, including the cell wall and membrane, chloroplasts, and starch granules. The effect may be attributed to the absorption of GO by lettuce cells, where it fixed Cd2+, thus reducing Cd2+ bioavailability, or to the improvement of Cd tolerance through regulation of lettuce metabolic pathways. Gaussian simulation analysis revealed that Cd caused significant changes in the GO molecule, resulting in detachment of an epoxy group from the GO carbon ring and breakage of OH bonds in hydroxyl groups, whereupon the oxygen freed from the OH bond formed a new bond with Cd. Collectively, these results indicate that foliar application of 30 mg/L GO can enhance the tolerance of lettuce to Cd, promote plant growth, and improve nutritional quality.