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Métodos Terapêuticos e Terapias MTCI
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1.
Vet Parasitol ; 200(1-2): 225-8, 2014 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-24332964

RESUMO

Two studies were carried out in order to test the effects of neem tree extracts (Azadirachta indica A. Juss) on sheep bot fly larvae (Oestrus ovis L. Diptera: Oestridae). First, aqueous extracts from neem seeds (ASNE) at 0, 5 y 10% (w/v) concentrations were tested on larval mortality in vitro. In a second study, the effect of oral administration with neem seed meal (0, 100 y 200mg/kg) and neem leaves (1% of diet) on number of larvae found at necropsy and larval development was evaluated in experimentally O. ovis-infected sheep. Results in Experiment 1 showed a significant (P<0.05) effect of ASNE on time to L1 mortality in a dosis-dependent manner. In Experiment 2, oral administration of seeds or leaves did not affect the number of larvae found at necropsy of the sheep, but interfered with larval development and there was a tendency to reduce larval weight at the end of the infection period (55d).


Assuntos
Azadirachta/química , Dípteros/efeitos dos fármacos , Miíase/veterinária , Extratos Vegetais/farmacologia , Extratos Vegetais/uso terapêutico , Doenças dos Ovinos/tratamento farmacológico , Animais , Larva/efeitos dos fármacos , Miíase/tratamento farmacológico , Folhas de Planta , Sementes/química , Ovinos , Resultado do Tratamento
2.
Int J Parasitol ; 27(1): 125-8, 1997 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-9076538

RESUMO

A culture medium in which 1st instar larvae of Oestrus ovis can survive for up to 2 months has been developed with Dulbecco's Modified Eagle's Medium (DMEM) pH 7.7, penicillin 100 U/ml, streptomycin 100 micrograms ml-1, gentamicin 10 micrograms ml-1 and foetal calf serum (50%) added. Larvae were incubated in flat plastic tissue culture bottles (3 ml of medium) in a 5% CO2 atmosphere at 37 degrees C in darkness. Subsequently an antiparasitic in vitro screening test was developed with moxidectin and closantel. These drugs were not as effective in vitro as in vivo. This might be due to the fact that they cause damage to parasites and host immune responses, then contribute to their death.


Assuntos
Antiparasitários/toxicidade , Dípteros/fisiologia , Inseticidas/toxicidade , Salicilanilidas/toxicidade , Animais , Antibacterianos , Avaliação Pré-Clínica de Medicamentos/métodos , Larva/efeitos dos fármacos , Larva/fisiologia , Macrolídeos/toxicidade
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