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1.
Molecules ; 29(7)2024 Apr 07.
Artigo em Inglês | MEDLINE | ID: mdl-38611943

RESUMO

Luteolin-7-O-ß-d-glucuronide (LGU) is a major active flavonoid glycoside compound that is extracted from Ixeris sonchifolia (Bge.) Hance, and it is a Chinese medicinal herb mainly used for the treatment of coronary heart disease, angina pectoris, cerebral infarction, etc. In the present study, the neuroprotective effect of LGU was investigated in an oxygen glucose deprivation (OGD) model and a middle cerebral artery occlusion (MCAO) rat model. In vitro, LGU was found to effectively improve the OGD-induced decrease in neuronal viability and increase in neuronal death by a 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and a lactate dehydrogenase (LDH) leakage rate assay, respectively. LGU was also found to inhibit OGD-induced intracellular Ca2+ overload, adenosine triphosphate (ATP) depletion, and mitochondrial membrane potential (MMP) decrease. By Western blotting analysis, LGU significantly inhibited the OGD-induced increase in expressions of receptor-interacting serine/threonine-protein kinase 3 (RIP3) and mixed lineage kinase domain-like protein (MLKL). Moreover, molecular docking analysis showed that LGU might bind to RIP3 more stably and firmly than the RIP3 inhibitor GSK872. Immunofluorescence combined with confocal laser analyses disclosed that LGU inhibited the aggregation of MLKL to the nucleus. Our results suggest that LGU ameliorates OGD-induced rat primary cortical neuronal injury via the regulation of the RIP3/MLKL signaling pathway in vitro. In vivo, LGU was proven, for the first time, to protect the cerebral ischemia in a rat middle cerebral artery occlusion (MCAO) model, as shown by improved neurological deficit scores, infarction volume rate, and brain water content rate. The present study provides new insights into the therapeutic potential of LGU in cerebral ischemia.


Assuntos
Lesões Encefálicas , Glucuronídeos , Luteolina , Animais , Ratos , Infarto da Artéria Cerebral Média/tratamento farmacológico , Simulação de Acoplamento Molecular , Transdução de Sinais , Proteínas Quinases
2.
Food Chem ; 135(2): 402-7, 2012 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-22868106

RESUMO

An ultra performance liquid chromatography (UPLC) method combined with solid phase extraction (SPE) sample pre-treatment was developed and validated for the rapid quantification of L-theanine in ready-to-drink (RTD) teas. UPLC analysis of twenty-seven RTD teas from the Chinese market revealed that the L-theanine levels in various types of RTD teas were significantly different. RTD green teas were found to contain highest mean L-theanine level (37.85±20.54 mg/L), followed by jasmine teas (36.60±12.08 mg/L), Tieguanying teas (18.54±3.46 mg/L) black teas (16.89±6.56), Pu-erh teas (11.31±0.90 mg/L) and oolong teas (3.85±2.27 mg/L). The ratio of total polyphenols content to L-theanine content could be used as a featured parameter for differentiating RTD teas. L-theanine in RTD teas could be a reliable quality parameter that is complementary to total polyphenols.


Assuntos
Camellia sinensis/química , Cromatografia Líquida de Alta Pressão/métodos , Glutamatos/análise , Polifenóis/análise , Extração em Fase Sólida/métodos , Chá/química , China , Cromatografia Líquida de Alta Pressão/instrumentação , Glutamatos/isolamento & purificação , Polifenóis/isolamento & purificação
3.
Zhongguo Zhong Yao Za Zhi ; 30(18): 1423-5, 2005 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-16381460

RESUMO

OBJECTIVE: To study on HPLC fingerprint of Propolis and to control it's quality. METHOD: The chromatographic fingerprints of seven samples from different producing areas were determined by RP-HPLC. RESULT: The chromatograms of Propolis from different producing areas were very similar. CONCLUSION: The quality of Propolis can be controlled by determination the HPLC fingerprint.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Materia Medica/química , Própole/química , Contaminação de Medicamentos , Flavonoides/análise , Controle de Qualidade , Reprodutibilidade dos Testes
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