RESUMO
In the present work, Dioscorea opposita Thunb polysaccharide-zinc (DPS-zinc) inclusion complex was synthesized for the first time and its anti-diabetes activities against Streptozotocin (STZ)-induced diabetic rats were assayed. The detail structural and morphological analysis of as-prepared DPS-zinc complex was done by using different spectroscopic and microscopic methods. The pharmacological study revealed that DPS-zinc presented a potent hypoglycemic effect on STZ-induced diabetic rats, decreased the glucose and insulin levels, reduced malondialdehyde (MDA) contents and increased SOD and T-AOC activities significantly in liver. The levels of T-CHO, TG and LDL-C in serum were dramatically decreased while HDL-C level was increased with the treatment of proposed inclusion complex. In brief, DPS-zinc could be considered as a potential candidate for developing an ingredient of functional foods for zinc supplements with the hypoglycemic action.
Assuntos
Dioscorea/química , Hipoglicemiantes/química , Hipoglicemiantes/farmacologia , Compostos Organometálicos/química , Compostos Organometálicos/farmacologia , Polissacarídeos/química , Zinco/química , Animais , Diabetes Mellitus Experimental/sangue , Diabetes Mellitus Experimental/tratamento farmacológico , Diabetes Mellitus Experimental/metabolismo , Feminino , Hipoglicemiantes/uso terapêutico , Insulina/sangue , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Malondialdeído/metabolismo , Camundongos , Compostos Organometálicos/uso terapêutico , Estresse Oxidativo/efeitos dos fármacos , Açúcares/sangueRESUMO
OBJECTIVE: Dioscorea opposita Thunb is the famous food and traditional medicine in China and it was rich in polysaccharides. Polysaccharides of Dioscorea Opposita Thunb possess immunoregulatory activity, free radical scavenging activity and anti-diabetic activity. A novel polysaccharide- iron(III) complex (CYPIC) was synthesized by using crude polysaccharide extracted from Dioscorea opposita Thunb. The component, structure, morphology and molecular weights of CYPIC were analysed, and the anti-anemia, acute toxicity and nonspecific immune regulating activities of CYPIC were assayed. The results showed that CYPIC could increase red blood cell count (RBC), hemoglobin (Hb), hematocrit (HCT), thymus and spleen index of mice with iron deficiency anemia (IDA). Although the structure and deeper mechanisms of CYPIC should be further studied, CYPIC has the potential to be used as an iron supplement for the treatment of iron deficiency anemia. CONCLUSION: The large scale industrial production was suggested due to the simple preparation processing of CYPIC.