Use of natural antioxidants for the correction of changes in general and local parameters of lipid peroxidation and antioxidant defense system during experimental eye burn.

The effect of natural antioxidants in grade III chemical eye burn was studied in experiments on rabbits at various stages of burn disease. The use of histochrome, Gingko Biloba, and diquertin in combination with complex drug therapy decelerated the decrease in the antioxidant potential of tear fluid and blood plasma. This treatment was also followed by a decrease in the concentration of end products of free radical oxidation.

[The role of selenium in the regulation of lipid peroxidation in biological membranes and glutathione peroxidase activity]. / Uchastie selena v reguliatsii perekisnogo okisleniia lipidov biomembran i aktivnost' glutationperoksidazy.

The antioxidative effect of selenium cannot be exclusively due to the functioning of the selenium-dependent glutathione peroxidase mechanism of utilization of various hydroperoxides. This hypothesis is based on the following experimental evidence. Selenium ions are readily incorporated into animal organs and tissues immediately after injection (2 hours) as well as into cell organelles and cytosol where they inhibit lipid peroxidation. The activity of glutathione peroxidase (EC 1.1.1.19) in rat liver and guinea pig cytosol is thereby unchanged but increases drastically after 12 hours reaching a maximum an the 3rd-4th day. The effectiveness of lipid peroxidation inhibition does not increase under these conditions. Although the glutathione peroxidase activity is absent in the nuclei and microsomes, exogenous selenium inhibits lipid peroxidation in these organelles. The activity of the rat liver cytosolic enzyme markedly exceeds that of its guinea pig counterpart. However, lipid peroxidation in guinea pig liver occurs less intensively than that in rat liver cytosol.

[Lipid peroxidation in the synaptosomal and mitochondrial fractions of separate brain structures in hypoxia]. / Perekisnoe okislenie lipidov v sinaptosomal'noi i mitokhondrial'noi fraktsiiakh otdel'nykh struktur mozga pri gipoksii.

The paper studies intensification of lipid peroxide oxidation in separate brain structures (the medulla oblongata, cerebellum, visual and sensomotor cortex), synaptosomal and mitochondrial fractions under hypoxia. It has been established that acute hypoxia increases accumulation of lipid peroxidation (LPO) products, hydroperoxide and malonyl dialdehyde. Intensification of LPO in synaptosomes and mitochondria is more pronounced as compared to the whole structures. Preliminary treatment with antioxidants (vitamin E and ionol) considerably suppressed LPO intensity under both hypoxia and hypoxia with reoxygenation. Intensification of LPO in synaptosomes and mitochondria is suggested to be the key point in structural-functional disturbances of the nervous system under hypoxia and ischemia.

[Dynamics of changes in the electroretinogram of isolated frog and turtle retinas during rhythmic photic stimulation in conditions of induced lipid peroxidation]. / Dinamika izmeneniia élektroretinogrammy izolirovannoi setchatki liagushek i cherepakh pri ritmicheskom svetovom razdrazhenii v usloviiakh indutsirovannogo lipopereokisleniia.

Comparative study of isolated retinas of frogs and turtles exposed to rhythmical photostimulation was conducted. It was shown that there are marked differences between the responses of isolated retinas of frogs and turtles to the rhythmical photostimulation. A considerable depression of the total amplitude of the rhythmical electroretinogram was observed under the conditions of induced lipid peroxidation. Introduction of a selenium compound to the superfusing medium increased to a certain extent both the single and rhythmical responses of isolated retinas of the animals.