An Integrated Approach to the Anti-Inflammatory, Antioxidant, and Genotoxic Potential of Portuguese Traditional Preparations from the Bark of Anacardium occidentale L.

Anacardium occidentale L. stem bark Traditional Herbal Preparations (AoBTHPs) are widely used in traditional medicine to treat inflammatory conditions, such as diabetes. The present study aims to evaluate the anti-inflammatory, antioxidant, and genotoxic potential of red and white Portuguese AoBTHPs. Using a carrageenan-induced rat paw edema model, a significant anti-edema effect was observed for all tested doses of white AoBTHP (40.2, 71.5, and 127.0 mg/kg) and the two highest doses of red AoB THP (71.5 and 127.0 mg/kg). The anti-edema effect of red AoBTHP's highest dose was much more effective than indomethacin 10 mg/kg, Trolox 30 mg/kg, and Tempol 30 mg/kg. In DPPH, FRAP, and TAC using the phosphomolybdenum method, both types of AoBTHPs showed similar antioxidant activity and no genotoxicity up to 5000 µg/plate in the Ames test. The LC-UV/DAD-ESI/MS fingerprint allowed the identification of gallic and protocatechuic acids as the two main marker compounds and the presence of catechin, epicatechin, epigallocatechin gallate, and ellagic acid in both AoBTHPs. The obtained results support the validation of red and white AoB and their THPs as anti-inflammatory agents and contribute to the possible development of promising new therapeutic options to treat inflammatory conditions.

Micromorphology and Chemical Studies on Anacardium occidentale L. Stem Bark as an Herbal Medicine.

The red and white types of Anacardium occidentale L. stem barks (AoB) are used in the Community of Portuguese Language Countries, including Portugal, to make traditional herbal preparations for the treatment of diabetes. This work aims to obtain the macroscopic, micromorphological, chemical, and purity data necessary to use both types of AoBs as medicinal plants safely. Macroscopically, a brown inner (red AoB) or a dark-brown inner surface (white AoB) was observed. Light and scanning electron microscopy showed that sclereid cells with thin cell walls were significantly larger (p < 0.001) in red AoB than in white AoB, but calcium oxalate druses and starch grain areas were significantly larger (p < 0.001) in white AoB than in red AoB. The chromatographic profiles (thin-layer chromatography and high-performance liquid chromatography) of both types of AoBs were characterized by the presence of gallic and protocatechuic acids and other phenolic derivatives. The condensed tannins were the major secondary metabolites class (143.69 ± 4.67 mg and 73.79 ± 4.46 mg catechin equivalents/g AoB in red and white types, respectively). The loss on drying and the total ash were, respectively, 7.07 ± 0.16% and 2.31 ± 0.18% in red AoB and 6.58 ± 0.16% and 1.94 ± 0.14% in white AoB. The obtained data are helpful in ensuring the quality of AoB as raw material for the pharmaceutical market.