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1.
Toxicol Ind Health ; 32(1): 106-17, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-24021433

RESUMO

The herbicide itself and the degradation products are highly toxic on biological systems. The aim of this study is to investigate the potential toxic effects of trifluralin (TRF) on the urinary system of male rats and to investigate the protective effects of resveratrol (RSV) in TRF-induced urinary system damage. A total of 35 male Wistar rats were randomly divided into: (1) control group, (2) sham group, (3) low dose TRF group (0.8 g/kg/day), (4) high dose TRF group (2 g/kg/day) and (5) high dose TRF + RSV group 10 mg/kg/day. RSV was administered for 21 days by intragastric gavage at a dose of 10 mg/kg/day after induction of TRF. Kidney, ureter and urinary bladder tissue was examined using light microscopy and ultrastructurally. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling was performed to detect apoptosis. Superoxide dismutase (SOD), glutathion peroxidase (GPx) and malondialdehyde (MDA) levels were also evaluated biochemically for oxidative stress parameters. Histological evaluation showed that TRF increases apoptosis and oxidative stress, causes histological tissue damages and biochemical changes in the kidneys but does not cause any damage to the ureter and bladder. Treatment with RSV significantly attenuated tissue damage in the urinary system of rats. Apopitotic cells were significantly decreased in the treatment group. Additionally, treatment with RSV decreased SOD and GPx levels and increased MDA levels in the kidney tissue of animals subjected to TRF. These results show that RSV can significantly minimize histological damage and biochemical differences in treating TRF-induced kidney injury in rats.


Assuntos
Antioxidantes/farmacologia , Estilbenos/farmacologia , Trifluralina/toxicidade , Sistema Urinário/efeitos dos fármacos , Animais , Apoptose/efeitos dos fármacos , Peso Corporal , Relação Dose-Resposta a Droga , Glutationa Peroxidase/metabolismo , Marcação In Situ das Extremidades Cortadas , Nefropatias/induzido quimicamente , Nefropatias/tratamento farmacológico , Masculino , Malondialdeído/metabolismo , Estresse Oxidativo/efeitos dos fármacos , Ratos , Ratos Wistar , Resveratrol , Superóxido Dismutase/metabolismo , Sistema Urinário/metabolismo , Sistema Urinário/patologia
2.
Iran J Allergy Asthma Immunol ; 11(4): 316-23, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23264408

RESUMO

Hedera helix is widely used to treat bronchial asthma for many years. However, effects of this herb on lung histopathology is still far from clear. We aimed to determine the effect of oral administration of Hedera helix on lung histopathology in a murine model of chronic asthma.BALB/c mice were divided into four groups; I (Placebo), II (Hedera helix), III (Dexamethasone) and IV (Control). All mice except controls were sensitized and challenged with ovalbumin. Then, mice in group I received saline, group II 100 mg/kg Hedera helix and group III 1 mg/kg dexamethasone via orogastic gavage once daily for one week. Airway histopathology was evaluated by using light and electron microscopy in all groups.Goblet cell numbers and thicknesses of basement membrane were found significantly lower in group II, but there was no statistically significant difference in terms of number of mast cells, thicknesses of epithelium and subepithelial smooth muscle layers between group I and II. When Hedera helix and dexamethasone groups were compared with each other, thickness of epithelium, subepithelial muscle layers, number of mast cells and goblet cells of group III were significantly ameliorated when compared with the group II. Although Hedera helix administration reduced only goblet cell counts and the thicknesses of basement membrane in the asthmatic airways, dexamethasone ameliorated all histopathologic parameters except thickness of basement membrane better than Hedera helix.


Assuntos
Remodelação das Vias Aéreas/efeitos dos fármacos , Antiasmáticos/farmacologia , Asma/tratamento farmacológico , Hedera , Pulmão/efeitos dos fármacos , Preparações de Plantas/farmacologia , Administração Oral , Animais , Antiasmáticos/administração & dosagem , Asma/imunologia , Asma/patologia , Membrana Basal/efeitos dos fármacos , Membrana Basal/ultraestrutura , Doença Crônica , Dexametasona/farmacologia , Modelos Animais de Doenças , Feminino , Células Caliciformes/efeitos dos fármacos , Células Caliciformes/ultraestrutura , Pulmão/imunologia , Pulmão/ultraestrutura , Camundongos , Camundongos Endogâmicos BALB C , Microscopia Eletrônica , Ovalbumina , Preparações de Plantas/administração & dosagem , Plantas Medicinais , Fatores de Tempo
3.
Allergy Asthma Proc ; 30(2): 186-91, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19118503

RESUMO

Platelet-activating factor (PAF) is an inflammatory mediator involved in the pathophysiology of asthma, suggesting a therapy antagonizing its effects may play a role in the disease treatment. The aim of the study was to determine the effects of Ginkgo biloba, a PAF antagonist, on lung histology. Thirty-five BALB/c mice were divided into five groups; A, B, C, D, and the control. All mice except controls were sensitized and challenged with ovalbumin. Mice in group A (placebo) received saline; group B received G. biloba, 100 mg/kg; group C received G. biloba, 150 mg/kg; and group D received dexamethasone, 1 mg/kg via orogastric gavage for 7 consecutive days. Chronic structural changes and airway remodeling were evaluated by using light and electron microscopy in all groups. Evaluation of lung histology indicated that the number of goblet cells, mast cells, thicknesses of epithelium, and basement membrane were significantly improved in groups B and C when compared with group A. There was no statistically significant difference in thicknesses of subepithelial smooth muscle between groups A, B, and C. When doses of G. biloba were compared with each other, only the number of goblet cells was significantly lower in group C than in group B. When G. biloba and dexamethasone groups were compared with each other, thicknesses of basement membrane and subepithelial smooth muscle were found to be lower in group D than in groups B and C. G. biloba alleviates all established chronic histological changes of lung except smooth muscle thickness in a mouse model of asthma.


Assuntos
Asma/tratamento farmacológico , Asma/patologia , Ginkgo biloba , Pulmão/efeitos dos fármacos , Pulmão/patologia , Fitoterapia , Extratos Vegetais/uso terapêutico , Mucosa Respiratória/efeitos dos fármacos , Mucosa Respiratória/patologia , Animais , Asma/imunologia , Doença Crônica , Modelos Animais de Doenças , Imunização Secundária , Injeções Intraperitoneais , Pulmão/imunologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Ovalbumina/imunologia , Folhas de Planta , Fator de Ativação de Plaquetas/antagonistas & inibidores , Mucosa Respiratória/imunologia
4.
Allergy Asthma Proc ; 29(5): 493-8, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18611306

RESUMO

Currently, asthma therapies are effective in reducing inflammation but airway remodeling is poorly responsive to these agents. New therapeutic options that have fewer side effects and reverse chronic changes in the lungs are essential. This study aimed to determine the efficacy of oral administration of ginseng on lung histopathology in a murine model of chronic asthma. BALB/c mice were divided into four groups: control, placebo, ginseng, and dexamethasone. All mice except those in the control group were sensitized and challenged with ovalbumin. Then, mice in the ginseng group were given 2 gr/kg per day of ginseng and mice in the dexamethasone group received 1 mg/kg per day of dexamethasone via orogastic gavage once daily for 1 week. Lung histopathology was evaluated by using light and electron microscopy in all groups. All of the chronic changes of airways in the ginseng group were significantly ameliorated when compared with the placebo group. When compared with the dexamethasone group, the ginseng group had significantly lower numbers of mast cell count. Thicknesses of basement membrane, epithelium, and subepithelial smooth muscle were not statistically different between the ginseng and dexamethasone groups. Goblet cell numbers were much more reduced in the dexamethasone group. Ginseng is effective in resolving the established chronic histopathological changes of the lungs in the murine model of asthma.


Assuntos
Asma/tratamento farmacológico , Panax , Fitoterapia , Animais , Asma/induzido quimicamente , Asma/patologia , Dexametasona/administração & dosagem , Feminino , Pulmão/patologia , Camundongos , Camundongos Endogâmicos BALB C , Ovalbumina/farmacologia
5.
Neurosci Lett ; 438(3): 265-9, 2008 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-18490106

RESUMO

It is known that the brain tissue is extremely sensitive to ischemia-reperfusion (IR) injury and therefore, brain ischemia and consecutive reperfusion result in neural damage and apoptosis. The proinflammatory cytokines such as tumor necrosis factor alfa (TNF-alpha) and interleukin-1 beta (IL-1beta) are produced during neurological disorders including cerebral ischemia. On the other hand, nerve growth factor (NGF), which is essential for the differentiation, survival and functions of neuronal cells in the central nervous system, regulate neuronal development through cell survival and cell death signaling. In the present study, we aimed to investigate the effect of selenium (Se) on prefrontal cortex and hippocampal damage in rats subjected to cerebral IR injury. Selenium was injected intraperitoneally at the doses of 0.625 mg/(kg day) after induction of IR injury. Prefrontal cortex and hippocampal damage was examined by cresyl-violet staining. Apostain and caspase-3 immune staining were used to detect apoptosis. TNF-alpha, IL-1beta and NGF levels were also evaluated. Histopathological evaluation showed that treatment with selenium after ischemia significantly attenuated IR-induced neuronal death in prefrontal cortex and hippocampal CA1 regions of rats. Apoptotic cells stained with apostain and caspase-3 were significantly decreased in treatment group when compared with the IR group. Additionally, treatment with selenium decreased the TNF-alpha and IL-1beta levels and increased the NGF levels in prefrontal cortex and hippocampal tissue of animals subjected to IR. The present results suggest that selenium is potentially a beneficial agent in treating IR-induced brain injury in rats.


Assuntos
Antioxidantes/uso terapêutico , Córtex Pré-Frontal/efeitos dos fármacos , Traumatismo por Reperfusão/tratamento farmacológico , Traumatismo por Reperfusão/patologia , Selênio/uso terapêutico , Animais , Caspase 3/metabolismo , Modelos Animais de Doenças , Hipocampo/efeitos dos fármacos , Marcação In Situ das Extremidades Cortadas , Interleucina-1beta , Masculino , Fatores de Crescimento Neural , Ratos , Ratos Wistar , Fator de Necrose Tumoral alfa
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