Bioremediation of uranium (â ¥) using a native strain Halomonas campaniensis ZFSY-04 isolated from uranium mining and milling effluent: Potential and mechanism.

A significant surge in the exploitation of uranium resources has resulted in considerable amounts of radioactive effluents. Thus, efficient and eco-friendly uranium removal strategies need to be explored to ensure ecological safety and resource recovery. In this study, we investigated the resistance of Halomonas campaniensis strain ZFSY-04, isolated from an evaporation pool at a uranium mine site, and its potential mechanism of uranium (Ⅵ) removal. The results showed that the strain exhibited unique uranium tolerance and its growth was not significantly inhibited under a uranium concentration of 700 mg/L. It had a maximum loading capacity of 865.40 mg/g (dry weight), achieved following incubation under uranium concentration of 100 mg/L, pH 6.0, and temperature 30 °C, for 2 h, indicating that the removal of uranium by the strain was efficient and rapid. Combined with kinetic, isothermal, thermodynamic, and microspectral analyses, the mechanism of uranium loading by strain ZFSY-04 was metabolism-dependent and diverse, including, physical and chemical adsorption on the cell surface, extracellular biomineralisation, intracellular bioaccumulation, and biomineralisation. Our results highlight the unique properties of indigenous strains, including high resistance, high efficiency, rapid uranium removal, and various uranium removal strategies, which make it suitable as a new tool for in situ bioremediation and uranium-contaminated environmental resource recovery.

Equilibrium, kinetics and thermodynamics of biosorption of U(VI) by Jonesia quinghaiensis strain ZFSY-01 isolated from the wastewater of a uranium mine.

The adsorption ability of a native Jonesia quinghaiensis strain ZFSY-01, a microorganism isolated from uranium tailing wastewater, to U(VI) in wastewater under different conditions was studied in this work. The results showed that 391.5 mg U/g and 78.3% of adsorption capacity and efficiency were achieved under an optimum adsorption condition, respectively. Especially, the adsorption capacity of this strain reached the maximum (Q=788.9 mg U/g) under 100 mg/L of strain dosage. Simultaneously, the linear regression coefficients for the used isothermal sorption model indicate that the biosorption process is compatible with the Freundlich isotherm, the Temkin isotherm and the Halsey isotherm model. Based on the fitted kinetic parameters, the data from the experiments fit well with models of pseudo-second-order kinetics and intraparticle diffusion, suggesting that the strain ZFSY-01 immobilized U(VI) by physical and chemical adsorption. In addition, thermodynamic parameters demonstrated that the sequestration of U(VI) by the strain is spontaneous and endothermic. Based on the above analysis, strain ZFSY-01 can effectively remove U(VI) ions from high- or low-concentration uranium-containing wastewater and is expected to become a promising biological adsorbent.

Remedial effect and operating status of a decommissioned uranium mill tailings (UMT) repository: A micro-ecological perspective based on bacterial community.

From a radioecological perspective, increasing attention has been paid to the long-term stabilisation of decommissioned uranium mill tailings (UMT) repositories. However, little is known about the evaluation of decommissioning and remedial effects of UMT repositories from a microecological perspective based on bacterial communities. Here, we analysed the distribution and structure of soil community assemblies along different vertical soil profiles in a decommissioned UMT repository and explored the impact of soil properties, including physicochemical parameters, metal(loid)s, and radionuclides, on the bacterial assemblage. We found that the α diversity of the bacterial community was unaffected by variations in different soil profiles and taxa were classified at the phylum level with small significant differences. In contrast, the bacterial community structure in and around the UMT repository showed significant differences; however, this difference was significantly affected by soil metal(loid)s and physicochemical properties rather than soil radionuclides. In addition, seven bacterial genera with significant differences between the inner and surrounding regions of the repository could be used as potential indicators to further investigate the remedial effects on soil environmental quality. These findings provide novel insights into the construction of an assessment system and in situ biomonitoring of UMT repositories from a microecological perspective based on bacterial communities.