Pharmacological administration of the isoflavone daidzein enhances cell proliferation and reduces high fat diet-induced apoptosis and gliosis in the rat hippocampus.

Soy extracts have been claimed to be neuroprotective against brain insults, an effect related to the estrogenic properties of isoflavones. However, the effects of individual isoflavones on obesity-induced disruption of adult neurogenesis have not yet been analyzed. In the present study we explore the effects of pharmacological administration of daidzein, a main soy isoflavone, in cell proliferation, cell apoptosis and gliosis in the adult hippocampus of animals exposed to a very high-fat diet. Rats made obese after 12-week exposure to a standard or high-fat (HFD, 60%) diets were treated with daidzein (50 mg kg(-1)) for 13 days. Then, plasma levels of metabolites and metabolic hormones, cell proliferation in the subgranular zone of the dentate gyrus (SGZ), and immunohistochemical markers of hippocampal cell apoptosis (caspase-3), gliosis (GFAP and Iba-1), food reward factor FosB and estrogen receptor alpha (ERα) were analyzed. Treatment with daidzein reduced food/caloric intake and body weight gain in obese rats. This was associated with glucose tolerance, low levels of HDL-cholesterol, insulin, adiponectin and testosterone, and high levels of leptin and 17ß-estradiol. Daidzein increased the number of phospho-histone H3 and 5-bromo-2-deoxyuridine (BrdU)-ir cells detected in the SGZ of standard diet and HFD-fed rats. Daidzein reversed the HFD-associated enhanced immunohistochemical expression of caspase-3, FosB, GFAP, Iba-1 and ERα in the hippocampus, being more prominent in the dentate gyrus. These results suggest that pharmacological treatment with isoflavones regulates metabolic alterations associated with enhancement of cell proliferation and reduction of apoptosis and gliosis in response to high-fat diet.

Las ratas tratadas con el suplemento dietético Vitamix® (Ceregumil® con vitaminas) muestran mayor resistencia física y capacidad antioxidante / Rats fed the dietary supplement vitamix® (Ceregumil® with vitamins) show greater physical resistance and antioxidant capacity

Objetivo: Vitamix® es un producto dietético compuesto por un extractohidroalcohólico de cereales y leguminosas con miel, glicerofosfato de calcio, vitaminas B y D selenio y flúor. El producto base, Ceregumil®, patentado en 1912, ha sido muy popular como reconstituyente, y los usuarios refieren una sensación de salud, resistencia a enfermedades o mayor predisposición para el trabajo o el ejercicio. Material y método: En el presente trabajo se analiza el efecto de Vitamix®, utilizado como suplemento alimenticio en ratas de laboratorio, en diversos parámetros fisiológicos y pruebas físicas. Periódicamente se realizaron hemogramas y se midieron la ingesta y el peso de los animales, así como las concentraciones sanguíneas de glucosa, triglicéridos, colesterol, transaminasas y malondialdehído, un producto de la lipoperoxidación. Se realizaron pruebas de resistencia física y sellevó a cabo un estudio histoquímico del hígado. Resultados: Los animales que tomaron Vitamix® tenían menor peso e ingesta en edades avanzadas, mostraban mayor capacidad antioxidante, mayor resistencia en la prueba del alambre y menor fatiga en la piscina de Morris. En este último caso, la mejoría era notable en los animales considerados de mal desempeño suplementados con Vitamix®. El resto de los parámetros medidos se mantuvieron estadísticamente similares a los de los controles y no se observaron alteraciones hepáticas de ningún tipo. Conclusiones: Este estudio supone una base científica y experimental para conocer el efecto de dichos complementos en los parámetrosfisiológicos (AU)

Objective: Vitamix® is a dietary product composed of a hydro-alcoholic extract of cereals and pulses with honey, calciumgly cerophosphate, vitamins B and D, selenium and fluoride. The basic product, Ceregumil®, patented in 1912, was highly popular as tonic and consumers reported a feeling of health, resistance to illness, and increased predisposition to work and exercise. Material and method: In the present study we analysed the effect of Vitamix® used as dietary supplement, on several physiological parameters in laboratoryrats. We periodically performed hemograms and measured intake and weight, as well as blood levels of glucose, triglycerides, cholesterol, transaminases and malondialdehyde, a lipoperoxidation product. Physical probes were performed and a histochemical study was done in the liver. Results: Rats fed with Vitamix® displayed lower intake and body weight in adult ages, showed and increased antioxidantactivity, higher resistance in the wire hang test and lower fatigue in the Morris pool, specially those specimens considered as bad performers supplemented with Vitamix®. The rest of the measured parameters remained similar to control and no hepatic alterations were found.Conclusions: This study supports as cientific basis to know the effect of these complements over physiologicalparameters (AU)

Rats fed the dietary supplement vitamix® (ceregumil® with vitamins) show greater physical resistance and antioxidant capacity.

OBJECTIVE: Vitamix® is a dietary product composed of a hydro-alcoholic extract of cereals and pulses with honey, calcium glycerophosphate, vitamins B and D, selenium and fluoride. The basic product, Ceregumil®, patented in 1912, was highly popular as tonic and consumers reported a feeling of health, resistance to illness, and increased predisposition to work and exercise. MATERIAL AND METHOD: In the present study we analysed the effect of Vitamix® used as dietary supplement, on several physiological parameters in laboratory rats. We periodically performed hemograms and measured intake and weight, as well as blood levels of glucose, triglycerides, cholesterol, transaminases and malondialdehyde, a lipoperoxidation product. Physical probes were performed and a histochemical study was done in the liver. RESULTS: Rats fed with Vitamix® displayed lower intake and body weight in adult ages, showed and increased antioxidant activity, higher resistance in the wire hang test and lower fatigue in the Morris pool, specially those specimens considered as bad performers supplemented with Vitamix®. The rest of the measured parameters remained similar to control and no hepatic alterations were found. CONCLUSIONS: This study supports a scientific basis to know the effect of these complements over physiological parameters.