RESUMO
Lectin from Chelidonium majus L. (CML) significantly stimulates the proliferation of human lymphocytes and has hemagglutination activity towards group B human erythrocytes and potent antimicrobial properties against multiresistant enterococci and staphylococci. In the present work we describe the effect of lectin from Chelidonium majus L on normal and cancercells in culture in vitro. The studies were performed on three types of cells: CHO, R2C and on normal mouse fibroblasts. Effects on the cultures were examined 24 h after addition of CML. Exposure to CML resulted in growth inhibition of CHO and R2C cells but not of fibroblasts. Moreover, evident apoptotic lesions were observed in CHO cells and less well marked apoptotic lesions in R2C cells. In contrast, only insignificant numbers of fibroblasts reacted to the applied lectin.
Assuntos
Antineoplásicos Fitogênicos/farmacologia , Lectinas/química , Lectinas/farmacologia , Papaver/química , Plantas Medicinais , Animais , Células CHO , Cromatografia Líquida de Alta Pressão , Cricetinae , DNA/química , Eletroforese em Gel de Poliacrilamida , Fibroblastos , Camundongos , Lectinas de Plantas , Espectrofotometria Ultravioleta , Células Tumorais CultivadasRESUMO
It has been recently recognized that lectins exhibit other activities besides hemagglutination. Previously we have found that purified lectin from Chelidonium majus showed DNase activity (Fik, Gozdzicka-Józefiak & Kedzia, 1995, Herba Polon. 41, 84-95). Comparison of lectin and DNase from the sap from leaves and roots of Chelidonium majus proved that both these compounds are composed of 24 kDa monomer subunits which have an identical N-terminal sequence but differ in amino-acid composition and degree of glycosylation. Possible interrelationship between lectin and DNase is discussed.
Assuntos
Desoxirribonucleases/metabolismo , Lectinas/química , Papaver/química , Papaver/enzimologia , Plantas Medicinais , Sequência de Aminoácidos , Cromatografia Líquida de Alta Pressão , Desoxirribonucleases/química , Desoxirribonucleases/isolamento & purificação , Glicoproteínas/química , Glicoproteínas/isolamento & purificação , Glicoproteínas/farmacologia , Testes de Hemaglutinação , Humanos , Lectinas/isolamento & purificação , Lectinas/farmacologia , Dados de Sequência Molecular , Fragmentos de Peptídeos/química , Folhas de Planta , Lectinas de Plantas , Raízes de PlantasRESUMO
New glycoprotein (CML) isolated from Chelidonium maius exhibits good antibacterial activity against methicillin resistant staphylococci and enterococci. It may constitute new antimicrobial agent against methicillin and vancomycin-resistant staphylococci as well as multiresistant enterococci.