Composto homeopático reduz a liberação de ânion superóxido pelas células mononucleares de ema (Rhea americana) / Homeopathic compound reduces the release of superoxide anion by mononuclear cells of rhea (Rhea americana)

The action of the "Stress Factor Ostrich (Arenales - Fauna and Flora)" was tested in the release of superoxide anion by cells in the peripheral blood of rhea (Rhea americana). Sixteen samples of 0.5mL of venous blood were collected through the jugular vein in the morning and placed in heparinized tubes. The leukocytes were separated into polymorphonuclear (PMN) and mononuclear (MN). The production of superoxide anion by phagocytes of peripheral blood was determined using the chromogen ferricytochrome C. There was a reduction of superoxide by MN cells in the presence of "Stress Factor Ostrich" indicating a positive influence of product against oxidative stress. Furthermore, future researches, such as the evaluation of other reactive oxygen intermediates and antioxidant enzymes, researches.

Composto homeopático reduz a liberação de ânion superóxido pelas células mononucleares de ema (Rhea americana) / Homeopathic compound reduces the release of superoxide anion by mononuclear cells of rhea (Rhea americana)

The action of the "Stress Factor Ostrich (Arenales - Fauna and Flora)" was tested in the release of superoxide anion by cells in the peripheral blood of rhea (Rhea americana). Sixteen samples of 0.5mL of venous blood were collected through the jugular vein in the morning and placed in heparinized tubes. The leukocytes were separated into polymorphonuclear (PMN) and mononuclear (MN). The production of superoxide anion by phagocytes of peripheral blood was determined using the chromogen ferricytochrome C. There was a reduction of superoxide by MN cells in the presence of "Stress Factor Ostrich" indicating a positive influence of product against oxidative stress. Furthermore, future researches, such as the evaluation of other reactive oxygen intermediates and antioxidant enzymes, researches.

Systemic inflammation and carotid diameter in obese patients: pilot comparative study with flaxseed powder and cassava powder.

BACKGROUND: Botanical omega-3 fatty acid (alphalinolenic acid/ALA) has been shown to alleviate the prothrombotic and proinflammatory profile of metabolic syndrome, however clinical protocols are still scarce. Aiming to focus an obese population, a pilot study was designed. METHODS: Morbidly obese candidates for bariatric surgery (n = 29, age 46.3 ± 5.2 years), 82.8% females (24/29), BMI 44.9 ± 5.2 kg/m², with C-reactive protein/CRP > 5 mg/L were recruited. Twenty were randomized and after exclusions, 16 were available for analysis. Flaxseed powder (60 g/day, 10 g ALA) and isocaloric roasted cassava powder (60 g/day, fat-free) were administered in a double-blind routine for 12 weeks. RESULTS: During flaxseed consumption neutrophil count decreased and fibrinogen, complement C4, prothrombin time and carotid diameter remained stable, whereas placebo (cassava powder) was associated with further elevation of those measurements. CONCLUSIONS: Inflammatory and coagulatory markers tended to exhibit a better outlook in the flaxseed group. Also large-artery diameter stabilized whereas further increase was noticed in controls. These findings raise the hypothesis of a less deleterious cardiovascular course in seriously obese subjects receiving a flaxseed supplement.

Distribution of NADPH-diaphorase cells in visual and somatosensory cortex in four mammalian species.

The distribution of the well-labeled nicotinamide adenine dinucleotide phosphate diaphorase (NADPHd) Type I neurons was evaluated in the isocortex of four mammalian species: the Didelphis opossum, the Monodelphis opossum, the rat and the marmoset. In Didelphis opossum, laminar distribution was examined in tangential and non-tangential sections. The density increases from superficial to deep layers of the gray matter. In rats' tangential sections, infragranular and supragranular layers have higher density than layer IV. Cell density measurements in the visual and the somatosensory cortices were compared in tangential sections from flattened hemispheres of the four species. Somatosensory areas were identified histochemically in rat (barrel fields) and marmoset (S1 and S2/PV). In the opossums, areas S1 and S2/PV were identified by multiunit recording. Except in the rat, primary visual cortex (V1) was labeled histochemically by NADPHd and/or cytochrome oxidase. In the four species, cell density in somatosensory cortex was significantly higher than in visual cortex. Taken together these results demonstrate that NADPHd Type I neurons are not homogeneously distributed in the isocortex of these mammals. In conclusion, the tangential distribution of Type I neurons in the sensory areas examined, but not its laminar distribution, was similar in the four species. Given that rats, marmosets and opossums are distantly related species, and that the latter are considered to have more 'generalized' brains, it is conceivable that this pattern of tangential distribution of Type I neurons is a general feature of mammalian isocortex.