RESUMO
The present research was carried out to look into therapeutic insight of biosynthesized silver nanoparticles (AgNPs) by leaf extract of Byttneria herbacea Roxb (BH). The analysis of biosynthesized BH-AgNPs by UV-visible spectroscopy shows an intense surface plasmon resonance (SPR) peak at 422 nm initially and 437 nm after 30 min which certainly reveals the formation of BH-AgNPs. Fourier Infra-red Spectroscopy (FT-IR) reveals that BH-AgNPs are biosynthesized by using different bioactive compounds like O-H stretch of free hydroxyl alcohol and phenols, N-H bond of primary amines present in the leaf extract. Transmission Electron Microscope (TEM) analysis revealed that BH-AgNPs are almost spherical in nature with an average size range from of 2 nm to 12 nm. The particle size analysis by Dynamic Light Scattering (DLS) reveals that the BH-AgNPs are poly-dispersed in nature with an average size of 8 nm ± 2 nm, with a negative zeta potential value of -21 mV which reveals the biosynthesized BH-AgNPs are very stable. The BH-AgNPs (Byttneria herbacea -AgNPs) revealed excellent free radical scavenging activity and exceptional antimicrobial activity. The anti-proliferative and cytotoxic studies in KB oral cancer cells revealed biosynthesized BH-AgNPs can employ as future novel therapeutic agents in cancer treatment and other biomedical applications.
Assuntos
Nanopartículas Metálicas , Neoplasias Bucais , Humanos , Nanopartículas Metálicas/química , Prata/química , Espectroscopia de Infravermelho com Transformada de Fourier , Extratos Vegetais/química , Antibacterianos/químicaRESUMO
The current investigation highlights the green synthesis of silver nanoparticles (AgNPs) by the insectivorous plant Drosera spatulata Labill var. bakoensis, which is the first of its kind. The biosynthesized nanoparticles revealed a UV visible surface plasmon resonance (SPR) band at 427 nm. The natural phytoconstituents which reduce the monovalent silver were identified by FTIR. The particle size of the Ds-AgNPs was detected by the Nanoparticle size analyzer confirms that the average size of nanoparticles was around 23 ± 2 nm. Ds-AgNPs exhibit high stability because of its high negative zeta potential (- 34.1 mV). AFM studies also revealed that the Ds-AgNPs were spherical in shape and average size ranges from 10 to 20 ± 5 nm. TEM analysis also revealed that the average size of Ds-AgNPs was also around 21 ± 4 nm and the shape is roughly spherical and well dispersed. The crystal nature of Ds-AgNPs was detected as a face-centered cube by the XRD analysis. Furthermore, studies on antibacterial and antifungal activities manifested outstanding antimicrobial activities of Ds-AgNPs compared with standard antibiotic Amoxyclav. In addition, demonstration of superior free radical scavenging efficacy coupled with potential in vitro cytotoxic significance on Human colon cancer cell lines (HT-29) suggests that the Ds-AgNPs attain excellent multifunctional therapeutic applications.
Assuntos
Drosera/metabolismo , Nanopartículas Metálicas/química , Prata/metabolismo , Anti-Infecciosos/farmacologia , Sequestradores de Radicais Livres/farmacologia , Química Verde , Células HT29 , Humanos , Nanopartículas Metálicas/uso terapêutico , Testes de Sensibilidade Microbiana , Tamanho da Partícula , Análise Espectral/métodos , Difração de Raios XRESUMO
In the present investigation, green synthesis of silver nanoparticles (AgNPs) was carried out using aqueous leaf extract of Argyreia nervosa. The results of the spectral characterisation have revealed that the surface Plasmon resonance band was observed at 421 nm confirms the formation of AgNPs. The Fourier Transform Infra-red Spectroscopy result shows the reduction of silver nitrate into AgNPs by the reduction of different functional groups. Transmission Electron Microscope analysis revealed that the particles are roughly spherical and poly-disperse in shape and size, the particles are within the size range of 10-55 nm. Dynamic Light Scattering revealed that the nanoparticles were also within the range of 10-50 nm, An-AgNPs have a high negative zeta potential value of -38.9 mV. An-AgNPs showed efficient free radical scavenging activity and showed excellent antimicrobial activity. Anti-proliferative and cytotoxic effect of An-AgNPs was carried out by MTT assay against KB oral cancer cells, the IC50 value of An-AgNPs is 58.64 µg/ml. The cell's growth is arrested at the G2/M phase, so the An-AgNPs activated the Caspase 3 pathway which leads to the Apoptosis of KB oral cancer cells. So it is concluded that the green synthesised An-AgNPs have manifold functions.
Assuntos
Nanopartículas Metálicas , Neoplasias Bucais , Antibacterianos/química , Antioxidantes/química , Antioxidantes/farmacologia , Linhagem Celular , Humanos , Nanopartículas Metálicas/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Prata/farmacologiaRESUMO
Biosynthesis of plant-mediated silver nanoparticles is gaining significant importance due to environmentally safe 'green method' and it is an efficient alternative method. In the present study, silver nanoparticles were synthesized by using root extract of Glycyrrhiza glabra an important medicinal plant. The AgNPs are characterized by spectral analysis; the surface plasmon resonance (SPR) peak of AgNPs showed maximum absorption at 445 nm. Fourier-transform infrared spectroscopy (FT-IR) data show that the O-H hydroxyl groups, carboxylic acids, ester and ether groups and C-O stretching of alcohols have been utilized in the formation of AgNPs. The X-ray powder diffraction (XRD) data reveal that the AgNPs are face-centered cubic (fcc) in structure. The size was determined by particle size analyzer and atomic force microscope (AFM); the results reveal that AgNPs were spherical in shape and the average grain size is determined as 41.5-46.5 nm. Transmission electron microscopy (TEM) micrographs obtained show that AgNPs were roughly spherical and well dispersed with the sizes ranging from 10 to 45 nm ± 5 nm. The biofabricated AgNPs are extremely stable due to its high negative zeta potential -34.1 mV which indicates that the nanoparticles are polydispered in nature. The cytotoxic studies of AgNPs on human CD34 +ve stem cells in microcarrier culture reveal excellent growth at different concentrations of biosynthesized AgNPs. This is the first report of microcarrier culture of CD34 +ve stem cells on biosynthesized AgNPs.
RESUMO
The enhancement of plant secondary metabolite production in cell suspension cultures through biotic or abiotic elicitation has become a potential biotechnological approach for commercialization or large-scale production of bioactive compounds. Gymnema sylvestre R.Br. is an important medicinal plant, rich in a group of oleanane triterpenoid saponins called gymnemic acid, well known for its anti-diabetic activity. Two endophytic fungal strains were isolated from the leaves of G. sylvestre and identified as Polyancora globosa and Xylaria sp. based on the PCR amplification and internal transcribed spacer (ITS 1-5.8S-ITS 2) sequencing of 18S rRNA gene. The process of elicitation of cell suspension cultures of G. sylvestre with dried powder of fungal mycelia (DPFM) and extracellular culture filtrate (ECF) of endophytic fungi consistently enhanced the accumulation of gymnemic acid and the DPFM was proved to be an effective elicitor when compared to the ECF. The DPFM elicited the gymnemic acid content in the range of 2.57-10.45-fold, while the ECF elicited the gymnemic acid content in the range of 2.39-7.8-fold. P. globosa, a novel and a rare endophytic fungal strain, has shown a great influence on the production of gymnemic acid. Cell suspension cultures elicited with DPFM of P. globosa produced higher amount of gymnemic acid content (124.23 mg/g dried cell weight) compared to the cultures elicited with DPFM of Xylaria sp. (102.24 mg/g DCW). But the cultures treated with consortium of DPFM of both fungi showed great influence on the production of gymnemic acid (139.98 mg/g DCW) than the cultures treated with DPFM alone. Similarly, cultures treated with consortium of ECF of both fungi produced more gymnemic acid content (94.86 mg/g DCW) compared with cultures treated with ECF of Xylaria sp. (77.93 mg/g DCW) and ECF of P. globosa (78.65 mg/g DCW) alone.
RESUMO
New and novel strategies are of recent interest in the development of silver nanoparticles. The plant extracts are eco-friendly, economical and cost effective for synthesis of nanoparticles. In this paper, we represent biofabrication of silver nanoparticles (AgNPs) using Andrographis paniculata and the synthesized AgNPs was monitored by ultra-violet visible spectroscopy (UV-Vis). The morphology and crystalline nature of AgNPs were determined from scanning electron microscopy (SEM) with Energy dispersive X-ray (EDX), X-ray diffraction patterns (XRD), Fourier transform-infrared spectroscopy (FT-IR). The size and the stability were detected by using Nanoparticle analyzer. The average size of the AgNPs was found to be 54 ± 2 nm and the Zeta potential was found to be -50.7 mV. The synthesized AgNPs have very good antifungal activity.
Assuntos
Andrographis/química , Antifúngicos/síntese química , Desenho de Fármacos , Nanopartículas Metálicas/química , Extratos Vegetais/química , Prata/química , Antifúngicos/química , Aspergillus niger/efeitos dos fármacos , Estabilidade de Medicamentos , Microscopia Eletrônica de Varredura , Oxirredução , Tamanho da Partícula , Penicillium/efeitos dos fármacos , Extratos Vegetais/isolamento & purificação , Espectrofotometria Ultravioleta , Propriedades de Superfície , Difração de Raios XRESUMO
Nanoparticles have been used to alter and improve the pharmacokinetic and pharmacodynamic properties of various types of drug molecules. The plant extracts are eco-friendly, economical and cost effective for synthesis of large scale of nanoparticles. In this paper we represent the synthesis of silver nanoparticles (AgNPs) from room dried leaves of Vinca rosea. The AgNPs were characterized by UV-vis spectroscopy. The AgNPs are crystalline in nature, were determined from scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX), X-ray diffraction patterns (XRD), and also the size of the NPs was calculated by using Hariba Nanoparticle analyzer and the stability was calculated by using the Zetapotential. The nanoparticles obtained from leaf extracts were of size 27±2 and 30±2 respectively and Zetapotential of AgNPs was found to be -63.1 mV, so it indicates the dispersion and stability. The synthesized AgNPs have very good antimicrobial activity.