Effectiveness of agricultural waste in the enhancement of biological denitrification of aquaculture wastewater.

Nitrogen pollution in aquaculture wastewater can pose a significant health and environmental risk if not removed before wastewater is discharged. Biological denitrification uses external carbon sources to remove nitrogen from wastewater; however, these carbon sources are often expensive and require significant energy. In this study, we investigated how six types of agricultural waste can be used as solid carbon sources in biological denitrification. Banana stalk (BS), loofah sponge (LS), sorghum stalk (SS), sweet potato stalk (SPS), watermelon skins (WS) and wheat husk (WH) were studied to determine their capacity to release carbon and improve denitrification efficiency. The results of batch experiments showed that all six agricultural wastes had excellent carbon release capacities, with cumulative chemical oxygen demands of 37.74-535.68 mg/g. During the 168-h reaction, the carbon release process followed the second-order kinetic equation and Ritger-Peppas equation, while carbon release occurred via diffusion. The kinetic equation fitting, scanning electron microscopy, and Fourier transform infrared spectroscopy results showed that LS had the lowest cm and the maximum t1/2 values and only suffered a moderate degree of hydrolysis. It also had the lowest pollutant release rate and cumulative chemical oxygen demand, as well as the most efficient removal of total phosphorous (TP) and total nitrogen (TN). Therefore, we concluded that LS has the lowest potential risk of excess carbon release and capacity for long-lasting and stable carbon release. The WS leachate had the highest TN contents, while the SPS leachate had the highest TP content. In the 181-h denitrification reaction, all six agricultural wastes completely removed nitrate and nitrite; however, SS had the highest denitrification rate, followed by LS, WH, BS, SPS, and WS (2.16, 1.35, 1.35, 1.34, 1.34, and 1.01 mg/(L·h), respectively). The denitrification process followed a zero-order and first-order kinetic equation. These results provide theoretical guidance for effectively selecting agricultural waste as a solid carbon source and improving the denitrification efficiency of aquaculture wastewater treatment.

[The effect of Ca2+ channel blocker on insulin secretion in rat pancreatic islet cells].

OBJECTIVE: To study the effect and mechanism of Ca2+ channel blocker on the insulin secretion in islet beta cells of rats. METHODS: The cells were divided into two groups: the low sugar concentration and high sugar concentration groups. These cells were examined with radioimmunoassay and fluorescence method and the effects of nifedipine (NIF), verapamil (VER), and diltiazem (DIL) on the contents of Ca2+ in the islet cells and the secretion volume of insulin at different concentrations were observed. RESULTS: In the low sugar concentration group, NIF, VER and DIL at concentration of 25, 50 and 100 microg/L respectively showed no effect on the contents of Ca2+ in islet cells and the insulin secretion (no statistical significance, P > 0.05). In the high sugar concentration group, these drugs had no inhibitory effect on insulin secretion at concentration of 25 microg/L. However, there was remarkable reduction in Ca2+ contents and insulin secretion when NIF was at the concentration of 50 and 100 microg/L, showing a relationship with dosage (P > 0.05, P > 0.01). VER, when given at 50 microg/L, showed a tendency of reduction in insulin secretion and there was a remarkable reduction at a dosage of 100 microg/L as compared with the control group (P > 0.05). When DIL was given at 100 microg/L, there was a noticeable difference in reduction of insulin secretion as compared with the control group (P > 0.05). CONCLUSION: High dosage of NIF, VER and DIL has an inhibitory effect on the entrance of extracellular Ca2+ into islet cells and thus reduces insulin secretion.