Dry-wet cycling area enhances soil ecosystem multifunctionality in the aquatic-terrestrial ecotones of the Caohai Lake in China.

The microbial need for nutrient resources can be assessed by soil extracellular enzymes and their stoichiometry. Changes in lake water levels affect land use and nutrient management in the aquatic-terrestrial ecotones of the lakeshore. However, the drivers of changes in microbial nutrient limitation under different inundation gradients in the lake's aquatic-terrestrial ecotones remain unclear. Here, based on vector analysis, we assessed microbial nutrient limitation by studying soil enzyme activities in four different inundation zones (heavy, moderate, mild, and non-inundation) in the aquatic-terrestrial ecotones of Caohai Lake. The findings indicate that inundation conditions significantly influenced the soil properties and enzyme activities. The mean nitrogen and phosphorus acquisition enzymes were higher in both moderate inundation (Mod-inu) and mild inundation (Mil-inu) zone soils, indicating rapid N and P turnover rates in these two zones. However, microorganisms had higher carbon requirements and higher enzyme C:N and vector lengths in heavily inundated compared to lightly inundated. Compared to the non-inundation zone, the microbial phosphorus limitation was found to be most severe in heavy inundation (Hea-inu) and Mod-inu zones. Decreased phosphorus limitation following the inundation weakens could be contributed to improving soil ecosystem multifunctionality. The alterations in the soil extracellular enzymes and stoichiometric characteristics in various inundation zones were primarily influenced by factors such as soil moisture content, available phosphorus, and nitrate nitrogen. Overall, the Mod-inu and Mil-inu zones can better maintain the multifunctionality of the aquatic and terrestrial ecosystems; special attention should be given to the microbial phosphorus limitation in the Hea-inu zone in order to effectively manage nutrients and restore soil ecosystems in the aquatic-terrestrial ecotones.

Increase phosphorus availability from the use of alfalfa (Medicago sativa L) green manure in rice (Oryza sativa L.) agroecosystem.

Alfalfa is a good green manure source, but its effect on rice growth has not been fully elucidated. Two green manure species, alfalfa and broad bean (Vicia faba L.), and two N fertilizer levels, alone or combination, were applied to a rice field. The results indicated that alfalfa had more pronounced effects on increasing soil labile phosphorus (P) fractions (including NaHCO3-Pi, NaOH-Pi), P uptake and soil enzyme activities (dehydrogenase, urease, acid phosphatase and ß-glucosidase) than broad bean and N fertilizer. The transformation of NaHCO3-Po to labile P regulated by alfalfa played a significant direct and indirect effect on grain yield. Although a much lower N input from alfalfa addition, a similar grain yield with N fertilizer treatment was achieved, and the integration of alfalfa with N fertilizer produced the highest grain yield and P availability, which was associated with the highest urease, acid phosphatase and ß-glucosidase activity in soil. These results indicate that alfalfa green manure had a great ability of increasing grain yield through enhancing P availability in rice paddy, which could give us a way to reduce N fertilizer application by enhancing P availability.