[The action of lactoperoxidase, lactoferrin and lactoglobulin on Shigella sonnei in an in-vivo experiment]. / Deistvie laktoperoksidazy, laktoferrina i laktoglobulina na Shigella sonnei v éksperimente in vivo.

The synergism of the protective action of lactoperoxidase, lactoferrin and lactoglobulin obtained from cow milk, as well as lactoferrin obtained from human milk, was shown in experiments on white mice, infected intranasally with S. sonnei and used as an experimental model. The combined administration of these preparations in doses, each one having no protective action, contributed to the elimination of the bacteria from the lungs and prevented the death of the animals. This phenomenon was found to be nonspecific and suitable for use in the development of preparations for passive immunization against a wide spectrum of microorganisms.

[Experimental study of the preventive properties of immune milk containing Shigella antibodies]. / Eksperimental'noe izuchenie preventivnykh svoistv immunnogo moloka, soderzhashchego protivoshigelleznye antitela.

The protective properties of the mammary gland secretions of cows immunized with Shigella sonnei into the udder were studied. As a model for this study the intranasal and intraperitoneal infection of white mice was used. Immune milk was found to have pronounced protective properties against S. sonnei. When introduced intraperitoneally, this milk protected the animals infected with S. sonnei from death. When introduced intranasally, it not only protected the animals from death, but perceptibly inhibited the development of the pathological process in the pulmonary tissue, preventing the multiplication of shigellae and accelerating the elimination of the infective agents from the lungs of the infected animals. As a rule, the degree of protective action was determined by the level of antibodies to shigellae in the substrate under test.

[Activity of bovine colostral IgG in the human digestive tract]. / Aktivnost' v pishchevaritel'nom trakte cheloveka korov'ego molozivnogo IgG.

The biological activity of cow IgG administered orally to 11 volunteers in the colostrum of cows vaccinated with Sh. sonnei was studied. At the same time the degree of immunologic protection was determined on the experimental model of pepsin fragments of this IgG active against Sh. sonnei. IgG, introduced orally, could be regularly found in gastric and intestinal juices, as well as in coprofiltrates. A decrease in the concentration of IgG and related antibodies was observed as this IgG moved down along the digestive tract: the maximum loss of biological activity occurred in the lower sections of the intestine. The products of pepsin hydrolysis of immune cow IgG ensure pronounced protection against Sh. sonnei. The preservation of the biological activity of IgG in the digestive secretions of adults receiving immune colostrum orally indicates the expediency of further studies in the field of passive enteral immunization for the prevention and treatment of acute intestinal diseases.