Preparation and physicochemical evaluation of casein/basil seed gum film integrated with guar gum/gelatin based nanogel containing lemon peel essential oil for active food packaging application.

In this study, the impact of adding lemon peel extract loaded nangel made of gelatin and guar gum in casein/basil seed gum film was examined. The films' mechanical, thermal stability, morphology, barrier, transmittance, antibacterial, antioxidant, and biocompatibility properties were investigated. The findings of this study demonstrated that incorporating the nanogels loaded with lemon peel extract in casein-basil seed gum (CB) film led to improve the properties of CB film including the mechanical properties, thermal stability, hydrophobicity, water vapor permeability, and water solubility, and also changed the color of the films, and slightly decreased the light transmission. The SEM images showed that in low percentages of nanogel, there is no significant difference in the roughness of the surface, but in higher percentages, an accumulation of nanoparticles has occurred on the surface. All films containing nanogel had good antioxidant properties and it was also observed that they had an inhibitory effect against Escherichia coli and Staphylococcus aureus bacteria. None of the films had toxicity for endothelial cells line for 72 h. Therefore, CB films containing nanogel loaded with lemon peel extract have good potential for food packaging.

Evaluation of therapeutic effects of nanofibrous mat containing mycophenolate mofetil on oral lichen planus: In vitro and clinical trial study.

Objectives: Recently, topical drug delivery system has gained increasing interest in the treatment of oral lesions. Lichen planus is a chronic inflammatory disease affecting mucous membranes and skin. The current study aimed to fabricate a drug delivery system containing mycophenolate mofetil for the treatment of oral lichen planus lesions. Methods: Firstly, a nanofibrous mat containing mycophenolate mofetil, zinc oxide nanoparticles, and aloe vera was designed and fabricated. The antimicrobial, cytocompatibility, anti-inflammatory, and antioxidative characteristics of fabricated scaffolds were evaluated. Then, this nanofibrous mat was applied to 12 patients suffering from bilateral erythematous/erosive Oral Lichen planus (OLP) lesions for 2 weeks. The treatment outcomes, including oral symptoms and lesion size, were compared with the routine topical treatment of these lesions; Triamcinolone ointment. Results: The characterization of nanofibrous mat approved the successful fabrication of scaffolds. The fabricated nanofibers showed notable antimicrobial activity. The amounts of TNF 𝛼, IL6, and reactive oxygen species (ROS) of stimulated human gingival fibroblasts were decreased after exposure to NFs/Myco/Alv/ZnO scaffolds. The clinical trial results demonstrated the same therapeutic effects compared to the commercial ointment, while the symptoms of patients were significantly improved in the mats group.Significance. Considering the successful results of this study, the application of nanofibrous mat can be a promising product for improving treatment outcomes of OLP.

Optimization and characterization of a novel tea tree oil-integrated poly (ε-caprolactone)/soy protein isolate electrospun mat as a wound care system.

A set of poly (ε-caprolactone)/soy protein isolate (PCL/SPI) mats with different ratios of PCL to SPI was fabricated using the electrospinning method. The mat with PCL to SPI ratio of 95:5 (PS 95:5) had the narrowest nanofibers, the highest percentage of porosity, the lowest swelling ratio, the least vapor transmission, and the slowest degradation rate among the prepared mats. The hemolysis assay indicated that all mats can be considered biocompatible biomaterials. In continue, three different weight ratios of tea tree oil (TTO) were loaded into the PS 95:5 mat. The release profiles illustrated that higher amounts of TTO could be released in an acidic environment. The antioxidant activity of the mats increased by the increase in their TTO content. The cell viability test, cell adhesion images, and live/dead assay of TTO-loaded mats affirmed that all fabricated mats were biocompatible. The scratch wound assay expressed that TTO accelerates the rate of wound closure. The TTO-loaded mats illustrated antibacterial activity against both Escherichia coli and Staphylococcus aureus bacteria. The obtained outcomes revealed that TTO-loaded PCL/SPI mats can be considered promising potential wound dressings.

Electrospun nanofiber based on Ethyl cellulose/Soy protein isolated integrated with bitter orange peel extract for antimicrobial and antioxidant active food packaging.

The present work was aimed to produce a novel bioactive nanofiber (NFs) based on Ethyl cellulose (EC), Soy protein isolated (SPI), and containing Bitter orange peel extract (BOPE) by electrospinning technology. The EC/SPI NFs were formulated with different weight ratios of 1:1, 2:1, and 1:2 denoted as ES11, ES21, and ES12, respectively, and investigated by several analyses. Based on the obtained results, the maximum hydrogen interactions between these two polymers, ES11 NFs offered a uniform morphology without bead with the diameter of 185.33 nm as a result of the compatibility of the polymer solutions of EC and SPI. Moreover, appropriate thermal stability was presented along with more porosity (78%), maximum water vapor transmission rate (657 g/m2.24h), good tensile stress (6.12 MPa), and acceptable water contact angel (82.3°). Therefore, ES11 NFs were selected as the optimal sample for incorporation of the BOPE as the antibacterial and antioxidant agent. According to the antioxidant activity test, the highest concentration (20% wt) of this extract increased the antioxidant activity of NF around 64.7% and also inhibited the growth of pathogenic bacteria (S. areus, and E. coli). Therefore, the ES11 electrospun NFs containing 20% BOPE can be a beneficial system to increase the safety and quality of foods.

Fabrication and characterization of novel antibacterial chitosan/dialdehyde guar gum hydrogels containing pomegranate peel extract for active food packaging application.

This study aimed to investigate synthesis and structural characteristics of the chitosan (CS) - modified dialdehyde guar gum (DAGG) hydrogel through the Schiff base reaction. The highest swelling capacity was achieved as about 12,000% of dry weight of the freeze-dried powder at CS: DAGG hydrogel with the mixing ratio of 30:70. The swelling ratio was not affected by changes in pH, which could be considered as an important property in the control of moisture in absorbent pad. The FTIR results indicated that the new amide groups have been formed at 1680 cm-1, which can be attributed to the covalent bond between the amide groups of CS and the aldehyde groups of GG. Based on a SEM image, the prepared hydrogel showed the porous structure so it verified the crosslinking formation between the two polymers. Rheological analyses confirmed that formation compact and porous structure led to some noteworthy improvements in the strength of hydrogel prepared with a high ratio of DAGG. The hydrogel loaded with 5% pomegranate peel extract (PPE) showed both good antioxidant (81.13%) and antimicrobial activities. The hydrogel was observed to have a good potential to be used as an antibacterial pad.

Pectin modification assisted by nitrogen glow discharge plasma.

This study is concerned with modification of high methoxy pectin (HMP) assisted by nitrogen AC glow discharge plasma. Intrinsic viscosity of plasma treated pectin (PTP) was measured as primary index for structural changes in pectin molecules at different plasma conditions. The intrinsic viscosity of pectin increased within 7 min of exposure to plasma treatment and then remained constant. According to Fourier Transform Infrared Spectrometry (FT-IR) analysis, the intensity of carboxylate peaks increased in PTP samples due to de-esterification of pectin. Moreover, by decreasing the degree of esterification (DE) in PTP samples, the results of FT-IR were confirmed. Based on high performance size-exclusion chromatography (HPSEC) analysis, the molecular weight of PTP reduced. PTP gel had higher storage and loss modulus and shorter linear viscoelastic region. Moreover, X-ray diffraction (XRD) studies indicated an increase of crystallinity in PTP due to the formation of hydrogen bonds. Results revealed that nitrogen AC glow discharge plasma had significant influence on physicochemical and functional properties of pectin. The result of this study showed AC glow discharge plasma has promising potential towards modification of pectin.

Improvement in the stability of betanin by liposomal nanocarriers: Its application in gummy candy as a food model.

Betanin is a red food pigment with health beneficial effects. Despite interest in the use of betanin, low bioaccessibility and oxidation limit its application. To overcome these restrictions, the betanin was loaded in liposomal nanocarriers with the encapsulation efficiency of 80.35 ±â€¯1%. To assess the efficiency of these nanocarriers, gummy candy was selected as a food model and its nutritional properties such as betanin stability and antioxidant activity were probed. The results showed that the betanin content and antioxidant activity of samples containing betanin-nanoliposomes were at least twice to those of samples containing free betanin. The tests show no differences in the sensory parameters of panelists for gummy candies fortified by betanin-loaded nanoliposomes compared to those fortified by betanin alone. As a result, the liposomal nanoparticles may be introduced as a suitable platform to stabilize and increase the bioavailability of betanin for applications in nutraceutical and medical fields.