RESUMO
Extraction methods like maceration, ultrasonication, vortex mixer, soxhlet extraction and microwave assisted extraction (MAE), were evaluated for the extraction of Camptothecin (CPT) from the leaves and stem of Tabernaemontana alternifolia, Tabernaemontana divaricata and Tabernaemontana citrifolia. The extracts were analyzed by high performance thin layer chromatography (HPTLC). The results show that the leaves of Tabernaemontana alternifolia exhibited highest yield of CPT as compared to the other species. MAE was the most efficient extraction method with CPT extraction yield of 0.154 ± 0.004% w/w from Tabernaemontana alternifolia leaves followed by Soxhlet extraction, sonication, maceration, and vortex extraction methods.
Assuntos
Tabernaemontana , Tabernaemontana/química , Folhas de Planta/química , Camptotecina , Extratos Vegetais/químicaRESUMO
The present communication deals with preparation of ß-cyclodextrin (ßCD) grafted hydroxypropylmethylcellulose (HPMC) hydrogel films using citric acid as crosslinking agent with the aim of improving the loading and achieving controlled release of hydrophobic weak base (ketoconazole). The hydrogel films were characterized by attenuated total reflectance-fourier transform infrared (ATR-FTIR) spectroscopy, solid state 13C-nuclear magnetic resonance (13C NMR) spectroscopy, thermal analysis and scanning electron microscopy (SEM). The films were evaluated for ßCD content, carboxyl content, swelling ratio, drug loading, drug release and hemolytic assay. ATR-FTIR spectra indicated crosslinking via ester formation whereas 13C NMR, thermal analysis and SEM confirmed ßCD grafting. The ßCD grafted hydrogel films with high carboxyl content showed maximum swelling and high drug loading. The presence of grafted ßCD helped to retard the release of ketoconazole from the hydrogel films. The hemolytic assay suggested the biocompatible nature of the hydrogel films. Altogether, ßCD grafted HPMC hydrogel films were found to be suitable for delivery of poorly soluble weak bases.