Establishment of UPLC-PDA Fingerprint of Baoyuantang Substance Benchmark and Identification of Its Chemical Constituents / 中国实验方剂学杂志

Objective:To establish the fingerprint of Baoyuantang substance benchmark， and to analyze and identify the common peaks. Method:A total of 15 batches of Baoyuantang substance benchmark were prepared， ultra performance liquid chromatography-diode array detector method （UPLC-PDA） was used to establish the fingerprint of the substance benchmark， and the methodology was developed. The chromatographic conditions were as follows：ACQUITY UPLC BEH Shield C₁₈ column （2.1 mm×100 mm， 1.7 μm）， mobile phase of 0.05% formic acid solution （A） and 0.05% formic acid acetonitrile solution （ B） for gradient elution （0-0.5 min， 5%-19%B； 0.5-6 min， 19%B； 6-10 min， 19%-27%B； 10-20 min， 27%-45%B； 20-20.1 min， 45%-95%B； 20.1-23 min， 95%B）， the flow rate of 0.4 mL·min^-1， the column temperature of 30 ℃， the detection wavelength at 203 nm and 260 nm， and the injection volume of 2 μL. Similarity evaluation system of traditional Chinese medicine fingerprint （2012 edition） was used to establish the fingerprint and generate the control fingerprint. The chemical constituents of Baoyuantang substance benchmark were identified by comparison of standard substances and UPLC-electrospray ionization tandem mass spectrometry （UPLC-ESI-MS/MS） with full information tandem mass spectrometry （MS^E） and scanning range of <italic>m</italic>/<italic>z</italic> 50-1 200. Result:The similarities of 15 batches of Baoyuantang substance benchmark were above 0.90 by comparing with the control fingerprint. There were 37 common peaks， 22 of which were identified through UPLC-ESI-MS/MS， including liquiritin， violanthin， ginsenoside Rg₁， ginsenoside Rb₁， ginsenoside Re and so on. These components were all from Astragali Radix， Ginseng Radix et Rhizoma， Zingiberis Rhizoma Recens and Glycyrrhizae Radix et Rhizoma. Conclusion:This method is accurate， stable and reliable， which will basically reflect the overall chemical composition characteristics of Baoyuantang， and it provides experimental basis for development of the granules of this famous classical formulas.

Industry Status and Development Strategies of Chinese Ethnic Medicine / 中国实验方剂学杂志

Ethnic medicine is an important part of traditional Chinese medicine, which has encountered many problems in the development process, such as the lack of effective inheritance of valuable experience and practice, weak basic research and lack of talents, serious destruction of ethnic medicine resources, uneven quality of medicinal materials, weak intellectual property protection, etc. To sum up, these seriously restrict the development of ethnic medicine. Here, the authors propose some corresponding suggestions according to these problems. Firstly, we should try our best to protect and mine relevant professional books for promoting national medicine culture, establish complete system of national and local standards, strengthen the construction of standardized planting bases and germplasm resource banks, build a well-known brand of ethnic medicine and give full play to the leading role of the brand. Secondly, we should strengthen basic research on ethnic medicine and build an integrated system of production-study-research. By integrating the strength of culture, scientific research, talents and industry, this paper hopes to promote the vigorous development of ethnic medicine.

Study on preparation and quality evaluation of standard decoction of Gentiana Radix based on traditional decocting process / 中草药

Objective To evaluate the extraction ratio, content determination, transfer ratio, and feature maps or fingerprints by establishing 15 batches of standard decoction of Gentiana Radix from five different places. Methods The Gentiana Radix was dissolved in water based on the traditional decoction process, the content of gentiopicroside was determined by UPLC-DAD, the transfer ratio, the extraction ratio, the fingerprints and the common peaks were evaluated by UPLC/Q-TOF-MS/MS. Results The determination of gentiopicroside of 15 batches of standard decoction was in the range of 1.57-2.81 mg/mL, the transfer ratio of gentiopicroside was in the range of 50.71%-67.70%, the average transfer ratio was 59.20%, and the standard deviation was 7.31%. The extraction ratio was in the range of 15.72%-25.52%, the average extraction ratio was 19.20% and the standard deviation was about 2.85%. Ten peaks of fingerprint were confirmed in this research, such as lilac glucoside, 1-(4-hydroxy-3-methoxy)-phenyl- 1,2,3-glycerol or isomer, loganate, secologanoside, gentiopicroside, naptho(2,3-c)furan-1(3H)-one-9-(acetyloxy)-6-methoxy-8- [(2,3,4-tri-O-acetyl-6-O-(2,3,4-tri-oacetyl-β-D-xylopyranosyl)-β-D-glucopyranosyl)oxy] or isomer, C46H54O28 (unknown compound), C46H54O27 (unknown compound), macrophylloside B, and isomacrophylloside. Conclusion The preparation method in this paper conforms to the traditional decoction method, the analyzed samples had representiveness, the batches fit the fingerprint research requirements, the content of quality evaluation covers a wide range and the data obtained are representative. It can be used for the standard preparation and quality evaluation of traditional Chinese medicine and providing basic data for the quality and the industrialization of dispensing granules.

Research strategies in standard decoction of medicinal slices / 中国中药杂志

This paper discusses the research situation of the standard decoction of medicinal slices at home and abroad. Combined with the experimental data, the author proposes that the standard decoction of medicinal slices is made of single herb using standard process which should be guided by the theory of traditional Chinese medicine, based on clinical practice and referred to modern extraction method with a standard process. And the author also proposes the principles of establishing the specification of process parameters and quality standards and established the basis of drug efficacy material and biological reference. As a standard material and standard system, the standard decoction of medicinal slices can provide standards for clinical medication, standardize the use of the new type of medicinal slices especially for dispensing granules, which were widely used in clinical. It can ensure the accuracy of drugs and consistency of dose, and to solve current supervision difficulties. Moreover the study of standard decoction of medicinal slices will provide the research on dispensing granules, traditional Chinese medicine prescription standard decoction and couplet medicines standard decoction a useful reference.