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1.
Domest Anim Endocrinol ; 80: 106726, 2022 07.
Artigo em Inglês | MEDLINE | ID: mdl-35413656

RESUMO

This study was undertaken for the development of novel techniques that are based on immunoneutralization of inhibin bioactivity to improve Holstein cow fertility. A series of 4 experiments were carried out on 2 farms that were located in subtropical or temperate regions, to test the effects of immunization against inhibin alpha subunit on cow fertility under varying degrees of heat stress conditions. Though immunization against inhibin alone improved conception rate (CR) after TAI moderately in cows under mild heat stress conditions, the treatment plus progesterone supplementation substantially enhanced CR in the range of 25 to 35 percentages from severe heat stress to comfortable weather conditions. There existed an additive effect between immunization against inhibin and progesterone supplementation that maximally enhanced CR. Further, immunization against inhibin increased both FSH and activin A concentrations in blood during both follicular and luteal phases. It also significantly increased blood concentrations of E2 in the follicular phase but decreased P4 concentrations during the early pregnancy. However, interferon-tau concentrations in blood around the time of pregnancy recognition were doubled in the inhibin immunized cows. In conclusion, immunization against inhibin plus P4 treatment enhances ovarian follicle and the subsequent early embryo developments that help to greatly improve the fertility of Holstein dairy cows.


Assuntos
Doenças dos Bovinos , Transtornos de Estresse por Calor , Infertilidade , Animais , Bovinos , Doenças dos Bovinos/prevenção & controle , Suplementos Nutricionais , Estradiol , Feminino , Hormônio Foliculoestimulante , Transtornos de Estresse por Calor/veterinária , Resposta ao Choque Térmico , Imunização/veterinária , Infertilidade/veterinária , Inibinas , Gravidez , Progesterona
2.
Artigo em Inglês | MEDLINE | ID: mdl-22784191

RESUMO

Desorption corona beam ionisation (DCBI), the relatively novel ambient mass spectrometry (MS) technique, was utilised to screen for illicit additives in weight-loss food. The five usually abused chemicals - fenfluramine, N-di-desmethyl sibutramine, N-mono-desmethyl sibutramine, sibutramine and phenolphthalein - were detected with the proposed DCBI-MS method. Fast single-sample and high-throughput analysis was demonstrated. Semi-quantification was accomplished based on peak areas in the ion chromatograms. Four illicit additives were identified and semi-quantified in commercial samples. As there was no tedious sample pre-treatment compared with conventional HPLC methods, high-throughput analysis was achieved with DCBI. The results proved that DCBI-MS is a powerful tool for the rapid screening of illicit additives in weight-loss dietary supplements.


Assuntos
Fármacos Antiobesidade/química , Suplementos Nutricionais/análise , Contaminação de Alimentos , Inspeção de Alimentos/métodos , Depressores do Apetite/análise , Depressores do Apetite/química , China , Cromatografia Líquida de Alta Pressão , Ciclobutanos/análise , Ciclobutanos/química , Fenfluramina/análise , Fenfluramina/química , Indicadores e Reagentes/análise , Indicadores e Reagentes/química , Legislação sobre Alimentos , Espectrometria de Massas , Fenolftaleína/análise , Fenolftaleína/química , Reprodutibilidade dos Testes , Inibidores Seletivos de Recaptação de Serotonina/análise , Inibidores Seletivos de Recaptação de Serotonina/química
3.
Aliment Pharmacol Ther ; 35(8): 865-75, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22360412

RESUMO

BACKGROUND: Management of recurrent Clostridium difficile-associated disease (CDAD), particularly in elderly patients, remains clinically challenging. Faecal transplantation (FT) may restore normal microbiota and break the cycle of recurrent CDAD. AIM: To critically appraise the clinical research evidence on the safety and effectiveness of FT compared with standard care in the treatment of patients with CDAD. METHODS: A comprehensive literature search was conducted by a research librarian to identify relevant studies published between 2000 and 2011. The Cochrane Library, PubMed, EMBASE, CINAHL, Biological Abstracts, BIOSIS Previews and Web of Science were searched using the following Medical Subject Headings (MeSH) terms and keywords, alone or in combination: Clostridium infections/Clostridium difficile/pseudomembranous/colitis/faeces/rectal/colon flora/gastrointestinal/nasogastric tube/enema/donor/transplant/infusion/bacteriotherapy/human probiotic infusion. Methodological quality of the included case series studies was assessed in terms of patient selection criteria, consecutive recruitment, prospective data collection, reporting of lost to follow-up, and follow-up rates. RESULTS: No controlled studies were found. Based on the weak evidence from seven full-text case series studies of 124 patients with recurrent/refractory CDAD, FT appears to be a safe and effective procedure. In most cases (83%) symptoms improved immediately after the first FT procedure, and some patients stayed diarrhoea free for several months or years. CONCLUSIONS: Although these results appear to be promising, the treatment effects of faecal transplantation cannot be determined definitively in the absence of a control group. Results from randomised controlled trials that compare faecal transplantation to oral vancomycin without or with a taper regimen will help to better define the role of faecal transplantation in the management of recurrent CDAD.


Assuntos
Clostridioides difficile/isolamento & purificação , Infecções por Clostridium/terapia , Enterocolite Pseudomembranosa/terapia , Fezes/microbiologia , Trato Gastrointestinal/microbiologia , Metagenoma/fisiologia , Infecções por Clostridium/microbiologia , Enterocolite Pseudomembranosa/microbiologia , Humanos , Interações Microbianas
4.
J. venom. anim. toxins incl. trop. dis ; 16(1): 155-165, 2010. ilus, tab
Artigo em Inglês | LILACS, VETINDEX | ID: lil-542437

RESUMO

In the current work, the courtship and mating of Scorpiops luridus Zhu Lourenço & Qi, 2005 (Euscorpiidae) from Xizang province (Tibet), China, were studied for the first time in the laboratory. Most of the mating behaviors in Scorpiops luridus are not remarkably different from those exhibited by other scorpions. However, for the first time a male pulling a female with its chelicerae to rapidly accomplish the sperm uptake was observed. Additionally, the sexual stinging behavior displayed by the male occurred in the initial stage, not during the promenade stage as previously described in several scorpion species. Through observation and analysis, we speculate that venom injection during sexual stinging is selective, possibly relying on the status shown by the stung scorpion (passive or aggressive). In order to clearly describe the process of courtship and mating, both sequences are represented in a flow chart, while the main behavior components of these processes were identified, analyzed and discussed.(AU)


Assuntos
Animais , Escorpiões , Comportamento Sexual Animal , Comportamento Animal , Laboratórios , Espermatozoides
5.
Phytochemistry ; 68(10): 1448-58, 2007 May.
Artigo em Inglês | MEDLINE | ID: mdl-17434191

RESUMO

To understand the factors contributing to estrogenic properties of extracts from the genus Epimedium L. (Berberidaceae), we performed taxonomic, genetic and chemical characterization on 37 specimens from 18 species and related these to estrogen receptor (ERalpha and ERbeta) bioactivity, as measured by reporter genes in stable human cells. Boot strap values derived from amplified fragment length polymorphisms indicated that specimens of E. koreanum, E. brevicornum, E. myrianthum, E. leishanense, and E. membranaceum were genetically distinct and this was supported by their very similar ERalpha activities. In contrast, specimens from E. pubescens and E. sagittatum were diverse both genetically, chemically and in terms of ERalpha and ERbeta bioactivities. Strikingly, a genetic cluster comprising six rare Epimedium species exhibited strongest ERalpha and ERbeta activity, and this bioactivity was positively correlated with content of trace flavonoid aglycones (kaempferol, apigenin, quercetin, luteolin and breviflavone B). In contrast, there was no association between estrogenic activity and the major flavonol glycoside constituents (icariin and epimedin A-C). Although they exhibited equally strong ERalpha and ERbeta activity, E. koreanum can be clearly differentiated from E. pubescens and E. brevicornum by genetic distance and its significantly lower content of epimedin C. Our morphologic, genetic, chemical and bioactivity profiling provide the basis for the production of extracts with reproducible estrogenic properties. Such reproducibility will be critical for the standardization of Epimedium-based products.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Epimedium/química , Fitoestrógenos/farmacologia , Células Cultivadas , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Epimedium/classificação , Epimedium/genética , Receptor alfa de Estrogênio/química , Receptor beta de Estrogênio/química , Flavonóis/química , Flavonóis/isolamento & purificação , Genes Reporter , Humanos , Filogenia , Fitoestrógenos/química , Fitoestrógenos/isolamento & purificação , Polimorfismo Genético
6.
Yao Xue Xue Bao ; 36(5): 386-9, 2001 May.
Artigo em Chinês | MEDLINE | ID: mdl-12584865

RESUMO

AIM: To discuss the intraspecific relationship in Magnolia officinalis and the genuineness of Cortex Magnoliae officinalis, and to find some DNA characters of certified "Houpo". METHODS: Thirty-three samples from eleven locations, which can represent most of the distribution of M. officinalis, were selected. The total DNA was extracted. Severty-four random primers were tried to get good amplification. RESULTS: One hundred and sixteen bands amplified from seventeen primers, were clustered by NTSYS-pc software. Three branches were obtained. Some distinctive primers and bands, which represent certified species or fine breed, were obtained also. CONCLUSION: 1) M. officinalis should be divided into three geographic clans instead of two subspecies or varieties, they are, a) typical officinalis, b) typical biloba and c) Middle type. This conclusion agrees with the leaf form and other characters. 2) The genetic difference between "Chuanpo" and "Wenpo" is evident and the difference is in correspondence with the quantities of their chemical constituents. So, the genetic difference is the main reason of the genuineness of Cortex Magnoliae officinalis. 3) These results may be used to establish DNA database for identification of Cortex Magnoliae officinalis.


Assuntos
DNA de Plantas/análise , Magnolia/genética , Plantas Medicinais/genética , Impressões Digitais de DNA , Contaminação de Medicamentos , Marcadores Genéticos , Magnolia/classificação , Casca de Planta/genética , Controle de Qualidade , Técnica de Amplificação ao Acaso de DNA Polimórfico , Especificidade da Espécie
7.
Sheng Li Ke Xue Jin Zhan ; 32(4): 293-7, 2001 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-12545853

RESUMO

Selenium (Se) is an essential trace element for animals. Selenocysteine (Sec), the 21st aminoacid, is a component of selenoproteins and has been founded in the active center of selenoenzymes. The functions of Se within the body have been primarily shown in the forms of selenoproteins, especially selenoenzymes. Incorporation of selenocysteine occurs on the basis of genetic expression and Se is the only trace element under direct genetic control. Recently, findings have shown that Se and selenocompounds conducted many other potential functions such as protection against inflammatory factors, inhibition of protein kinase C (PKC), stimulation of MAP kinase (mitogen activated protein kinase/myelin basic protein kinase) and S6 kinase (ribosomal S6 protein kinase), regulation of the immune system and interaction with other elements and vitamins etc, suggesting that the roles of Se in human health may be more diverse than previously suspected.


Assuntos
Selênio/fisiologia , Selenocisteína/fisiologia , Humanos , Proteínas/fisiologia , Selenoproteínas , Tiorredoxina Dissulfeto Redutase/fisiologia
8.
J Chromatogr A ; 870(1-2): 501-10, 2000 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-10722108

RESUMO

A method for the screening and analysis of biologically active compounds in traditional Chinese medicine is proposed. Affinity chromatography using a human serum albumin (HSA) stationary phase was applied to separate and analyze the bioactive compounds from Artemisia capillaris Thunb. Five major peaks and several minor peaks were resolved based on their affinity to HSA, two of them were identified as scoparone (SCO, 6,7-dimethoxycoumarin) and capillarisin (CAP). CAP shows a much higher affinity to HSA than SCO. The effects of acetonitrile concentration, eluent pH, phosphate concentration and temperature on the retention behaviors of several major active components were also investigated, and it was found that hydrophobicity and eluent pH play major roles in changing retention values. The results demonstrate that the affinity chromatography with a HSA stationary phase is an effective way for analyzing and screening biologically active compounds in traditional Chinese medicine.


Assuntos
Artemisia/química , Cromatografia de Afinidade/métodos , Plantas Medicinais , Albumina Sérica/química , Acetonitrilas/química , Concentração de Íons de Hidrogênio , Medicina Tradicional Chinesa , Concentração Osmolar , Fitoterapia , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Espectrofotometria Ultravioleta , Temperatura
9.
Zhongguo Zhong Yao Za Zhi ; 24(4): 238-9, 256-inside back cover, 1999 Apr.
Artigo em Chinês | MEDLINE | ID: mdl-12205950

RESUMO

OBJECTIVE: To study the cerebral protective effects of some compounds isolated from traditional Chinese herbs. METHOD: Laser microcirculation dynamic analyzer and cultured rat cortical neurons were used. RESULT: Pueraria lobata flavonoids(LPF), Panax notoginseng saponins(PNS), oxymatrine, anisodamine and berberine could dilate microvessels and increase the cerebral blood flow of anesthetized mice's meninges. LPF, PNS, oxymatrine, and anisodamine also accelerated the flow of blood. Oxymatrine, like anisodamine, increased quantities of erythrocytes in high frequency movements. PNS could protect cultured rat cortical neurons from glutamate neurotoxicity. CONCLUSION: LPF, PNS, oxymatrine, anisodamine and berberine have certain protective effects on brain, but differ in mechanisms of action.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Fármacos Neuroprotetores/farmacologia , Plantas Medicinais/química , Alcaloides/farmacologia , Animais , Berberina/farmacologia , Encéfalo/irrigação sanguínea , Circulação Cerebrovascular/efeitos dos fármacos , Flavonoides/isolamento & purificação , Flavonoides/farmacologia , Ginsenosídeos/isolamento & purificação , Ginsenosídeos/farmacologia , Camundongos , Microcirculação/efeitos dos fármacos , Panax/química , Pueraria/química , Quinolizinas , Ratos , Alcaloides de Solanáceas/farmacologia
10.
J Food Prot ; 61(1): 98-102, 1998 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-9708260

RESUMO

Mechanisms of resistance to infection by the fungus Aspergillus flavus and accumulation of aflatoxin were studied in kernels of resistant (GT-MAS:gk, Mp420) and susceptible ( Pioneer 3154, Deltapine G-4666) corn genotypes. Proteins from kernel extracts of corn genotypes were analyzed by several methods of polyacrylamide gel electrophoresis. Consistent differences in protein profiles were detected among genotypes. Several proteins were unique to or present in greater concentration in resistant genotypes, whereas others were present only in susceptible genotypes. Extracts of resistant kernels showed markedly greater antifungal activity against A. flavus than did susceptible kernel extracts. Results from the present study suggest a role for kernel proteins in resistance to A. flavus infection and aflatoxin contamination in corn genotypes.


Assuntos
Aspergillus flavus/efeitos dos fármacos , Extratos Vegetais/farmacologia , Proteínas de Plantas/análise , Zea mays/química , Zea mays/microbiologia , Aspergillus flavus/fisiologia , Microbiologia de Alimentos , Genótipo , Zea mays/genética
11.
J Cell Biochem ; 68(4): 500-10, 1998 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-9493912

RESUMO

The multifunctional transcription factor YY1 is associated with the nuclear matrix. In osteoblasts, the interaction of several nuclear matrix-associated transcription factors with the bone specific osteocalcin gene contributes to tissue-specific and steroid hormone-mediated transcription. A canonical nuclear matrix targeting signal (NMTS) is present in all members of the AML/CBFbeta transcription factor family, but not in other transcription factors. Therefore, we defined sequences that direct YY1 (414 amino acids) to the nuclear matrix. A series of epitope tagged deletion constructs were expressed in HeLa S3 and in human Saos-2 osteosarcoma cells. Subcellular distribution was determined in whole cells and nuclear matrices in situ by immunofluorescence. We demonstrated that amino acids 257-341 in the C-terminal domain of YY1 are necessary for nuclear matrix association. We also observed that sequences within the N-terminal domain of YY1 permit weak nuclear matrix binding. Our data further suggest that the Gal4 epitope tag contains sequences that affect subcellular localization, but not targeting to the nuclear matrix. The targeted association of YY1 with the nuclear matrix provides an additional level of functional regulation for this transcription factor that can exhibit positive and negative control.


Assuntos
Nucléolo Celular/metabolismo , Proteínas de Ligação a DNA/química , Proteínas de Ligação a DNA/metabolismo , Matriz Nuclear/metabolismo , Fatores de Transcrição/química , Fatores de Transcrição/metabolismo , Transcrição Gênica , Animais , Compartimento Celular , Linhagem Celular , Proteínas de Ligação a DNA/genética , Fatores de Ligação de DNA Eritroide Específicos , Imunofluorescência , Células HeLa , Humanos , Camundongos , Oligonucleotídeos/genética , Plasmídeos/genética , Deleção de Sequência , Relação Estrutura-Atividade , Fatores de Transcrição/genética , Ativação Transcricional , Transfecção , Fator de Transcrição YY1
12.
Biochemistry ; 36(47): 14447-55, 1997 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-9398163

RESUMO

Proteins of the ATF/CREB class of transcription factors stimulate gene expression of several cell growth-related genes through protein kinase A-related cAMP response elements. The promoter activity of cell cycle regulated histone H4 genes is regulated by at least four principal cis-acting elements which mediate G1/S phase control and/or enhancement of transcription during the cell cycle. Using protein-DNA interaction assays we show that the H4 promoter contains two ATF/CREB recognition motifs which interact with CREB, ATF1, and ATF2 but not with ATF4/CREB2. One ATF/CRE motif is located in the distal promoter at the nuclear matrix-associated Site IV, and the second motif is present in the proximal promoter at Site I. Both ATF/CRE motifs overlap binding sequences for the multifunctional YY1 transcription factor, which has previously been shown to be nuclear matrix associated. Subnuclear fractionation reveals that there are two ATF1 isoforms which appear to differ with respect to DNA binding activity and partition selectively between nuclear matrix and nonmatrix compartments, consistent with the role of the nuclear matrix in regulating gene expression. Site-directed mutational studies demonstrate that Site I and Site IV together support ATF1- and CREB-induced trans-activation of the H4 promoter. Thus, our data establish that ATF/CREB factors functionally modulate histone H4 gene transcription at distal and proximal promoter elements.


Assuntos
Ciclo Celular/fisiologia , Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/metabolismo , Regulação da Expressão Gênica , Histonas/biossíntese , Histonas/genética , Matriz Nuclear/metabolismo , Regiões Promotoras Genéticas , Fatores de Transcrição/metabolismo , Ativação Transcricional , Fator 1 Ativador da Transcrição , Fator 2 Ativador da Transcrição , Sequência de Aminoácidos , Animais , Sequência de Bases , Sítios de Ligação , Células COS , Sequência Consenso , Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/química , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Proteínas de Ligação a DNA/química , Proteínas de Ligação a DNA/metabolismo , Fatores de Ligação de DNA Eritroide Específicos , Fase G1 , Células HeLa , Humanos , Zíper de Leucina , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Oligodesoxirribonucleotídeos , Proteínas Recombinantes/metabolismo , Reticulócitos/metabolismo , Fase S , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Fatores de Transcrição/química , Transfecção , Fator de Transcrição YY1 , Dedos de Zinco
13.
Zhongguo Zhong Yao Za Zhi ; 22(5): 268-70, 318, 1997 May.
Artigo em Chinês | MEDLINE | ID: mdl-11038955

RESUMO

The pollens of 11 species and one variety of Epimedium grown in Guizhou Province have been observed by SEM. The morphological characters (especially ektexine sculpture) of the pollens are described and the correlation among pollen morphology plant forms and chemical constituents is discussd.


Assuntos
Plantas Medicinais/ultraestrutura , Pólen/ultraestrutura , Magnoliopsida/ultraestrutura , Microscopia Eletrônica de Varredura
14.
Proc Natl Acad Sci U S A ; 94(1): 121-6, 1997 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-8990171

RESUMO

The responsiveness of genes to steroid hormones is principally mediated by functional interactions between DNA-bound hormone receptors and components of the transcriptional initiation machinery, including TATA-binding protein, TFIIB, or other RNA polymerase II associated factors. This interaction can be physiologically modulated by promoter context-specific transcription factors to facilitate optimal responsiveness of gene expression to hormone stimulation. One postulated regulatory mechanism involves the functional antagonism between hormone receptors and nonreceptor transcription factors interacting at the same hormone response element. Here we demonstrate that the multifunctional regulator YY1 represses 1,25-dihydroxyvitamin D3 (vitamin D)-induced transactivation of the bone tissue-specific osteocalcin gene. We identify YY1 recognition sequences within the vitamin D response element (VDRE) of the osteocalcin gene that are critical for YY1-dependent repression of vitamin D-enhanced promoter activity. We show that YY1 and vitamin D receptor (VDR)/retinoid X receptor heterodimers compete for binding at the osteocalcin VDRE. In addition, we find that YY1 interacts directly with TFIIB, and that one of the two tandemly repeated polypeptide regions of TFIIB spanning the basic domain is responsible for this interaction. TFIIB and VDR can also interact directly, and these factors synergize to mediate transactivation. Our results suggest that YY1 regulates vitamin D enhancement of osteocalcin gene transcription in vivo by interfering with the interactions of the VDR with both the VDRE and TFIIB.


Assuntos
Osso e Ossos/metabolismo , Proteínas de Ligação a DNA/metabolismo , Osteocalcina/genética , Receptores de Calcitriol/metabolismo , Receptores do Ácido Retinoico/metabolismo , Fatores de Transcrição/metabolismo , Ativação Transcricional , Vitamina D/farmacologia , Animais , Sítios de Ligação , Ligação Competitiva , Osso e Ossos/efeitos dos fármacos , Fatores de Ligação de DNA Eritroide Específicos , Modelos Genéticos , Proteínas Nucleares/metabolismo , Osteocalcina/biossíntese , Osteossarcoma , Ratos , Proteínas Recombinantes/metabolismo , Receptores X de Retinoides , Fator de Transcrição TFIIB , Transfecção , Células Tumorais Cultivadas , Fator de Transcrição YY1
15.
Zhongguo Zhong Yao Za Zhi ; 21(10): 614-6, 640, 1996 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-9772630

RESUMO

Five flavonoids were isolated from Epimedium wanshanense and identified as sagittatoside B, anhydroicaritin-3-O-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L-rhamnopyranoside, sagittatoside A, ikarisoside B and desmethylanhydroicaritin-3-O-alpha-L-rhamnopyranosyl-(1-->2)-alpha-L- rhamnopyranoside by means of IR, UV, 1HNMR, 13CNMR, MS and chemical evidence. They were obtained from this plant for the first time.


Assuntos
Medicamentos de Ervas Chinesas/química , Flavonoides/isolamento & purificação , Magnoliopsida/química , Flavonoides/química , Estrutura Molecular
16.
Zhongguo Zhong Yao Za Zhi ; 21(9): 523-5, 574, 1996 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-9772637

RESUMO

By means of RP-HPLC, nine major flavonoids in different parts of five Epimedium plants incorporated in the Chinese Pharmacopoeia (1990 edn.) were analyzed. The result shows that the total contents of nine flavonoids in four species were rhizome and roots > leaves > stems. Nevertheless, in terms of composition of the main constituents and relative contents of the five species, the leaves are similar to the stems but different from the rhizome and roots. The difference may be reflected in their pharmaceutical effects and thus deserves attention.


Assuntos
Flavonoides/análise , Magnoliopsida/química , Plantas Medicinais/química , Cromatografia Líquida de Alta Pressão/métodos , Magnoliopsida/classificação , Folhas de Planta/química , Raízes de Plantas/química , Caules de Planta/química , Plantas Medicinais/classificação
17.
Zhongguo Zhong Yao Za Zhi ; 21(6): 353-5, 383, 1996 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-9388923

RESUMO

Six chemical constituents were isolated from the whole plant of Epimedium fargesii and identified as epimedoside A, ikarisosids C, epimedin B, epimedin C, icariin and hyperin by means of UV, IR, 1H-NMR, 13C-NMR and MS spectral analysis. They were isolated from this plant for the first time.


Assuntos
Flavonoides/isolamento & purificação , Plantas Medicinais/química , Flavonoides/química
18.
Zhonghua Nei Ke Za Zhi ; 35(1): 15-8, 1996 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-9275639

RESUMO

The present study investigated effects of Radix angelicae sinensis (RAS) on systemic and portal hemodynamics in cirrhotic patients with portal hypertension. The results demonstrated that, after intravenous injection of the agent (20 mg.kg-1.min-1), systemic hemodynamics (heart rate, mean arterial pressure, inferior vena cava pressure, systemic vascular resistance, cardiac output, cardiac index) remained unchanged, but portal hemodynamics signicantly improved, wedged hepatic venous pressure (WHVP) (3.79 +/- 0.76 kPa vs 2.72 +/- 0.89 kPa, P < 0.01) and hepatic venous pressure gradient (HVPG) (2.88 +/- 0.73 kPa vs 1.78 +/- 0.68 kPa, P < 0.01) reduced by 29.40 and 35.78 percent respectively. However, anti-portal hypertensive effect of RAS was inverse proportion to the basical level of WHVP or HVPG in the cirrhotics. We conclude that RAS can effectively decrease portal pressure and yet gives no influence on systemic hemodynamics in the cirrhotic patients with portal hypertension.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Hemodinâmica/efeitos dos fármacos , Hipertensão Portal/fisiopatologia , Cirrose Hepática/fisiopatologia , Pressão na Veia Porta/efeitos dos fármacos , Adulto , Feminino , Humanos , Cirrose Hepática/terapia , Masculino , Pessoa de Meia-Idade
19.
Proc Natl Acad Sci U S A ; 92(23): 10526-30, 1995 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-7479833

RESUMO

NMP-1 was initially identified as a nuclear matrix-associated DNA-binding factor that exhibits sequence-specific recognition for the site IV regulatory element of a histone H4 gene. This distal promoter domain is a nuclear matrix interaction site. In the present study, we show that NMP-1 is the multifunctional transcription factor YY1. Gel-shift and Western blot analyses demonstrate that NMP-1 is immunoreactive with YY1 antibody. Furthermore, purified YY1 protein specifically recognizes site IV and reconstitutes the NMP-1 complex. Western blot and gel-shift analyses indicate that YY1 is present within the nuclear matrix. In situ immunofluorescence studies show that a significant fraction of YY1 is localized in the nuclear matrix, principally but not exclusively associated with residual nucleoli. Our results confirm that NMP-1/YY1 is a ubiquitous protein that is present in both human cells and in rat osteosarcoma ROS 17/2.8 cells. The finding that NMP-1 is identical to YY1 suggests that this transcriptional regulator may mediate gene-matrix interactions. Our results are consistent with the concept that the nuclear matrix may functionally compartmentalize the eukaryotic nucleus to support regulation of gene expression.


Assuntos
Núcleo Celular/química , Proteínas de Ligação a DNA/isolamento & purificação , Fatores de Transcrição/isolamento & purificação , Animais , Especificidade de Anticorpos , Sequência de Bases , Sítios de Ligação , Western Blotting , Compartimento Celular , Nucléolo Celular/química , Nucléolo Celular/ultraestrutura , Núcleo Celular/ultraestrutura , Reações Cruzadas , DNA/metabolismo , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/imunologia , Proteínas de Ligação a DNA/metabolismo , Fatores de Ligação de DNA Eritroide Específicos , Células HeLa , Humanos , Dados de Sequência Molecular , Matriz Nuclear/química , Oligodesoxirribonucleotídeos/metabolismo , Ligação Proteica , Ratos , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , Fatores de Transcrição/genética , Fatores de Transcrição/imunologia , Fatores de Transcrição/metabolismo , Fator de Transcrição YY1
20.
J Biol Chem ; 270(28): 16529-35, 1995 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-7622457

RESUMO

Iron regulatory proteins (IRP1 and IRP2) are RNA-binding proteins that bind to stem-loop structures, termed iron-responsive elements (IREs), present in either the 5'- or 3'-untranslated regions of specific mRNAs. The binding of IRPs to 5'-IREs inhibits translation of mRNA, whereas the binding of IRPs to 3'-IREs stabilizes mRNA. To study the structure and regulation of IRP2, we isolated cDNAs for rat and human IRP2. The derived amino acid sequence of rat IPR2 is 93% identical with that of human IRP2 and is present in lower eukaryotes, indicating that IRP2 is highly conserved. IRP1 and IRP2 share 61% overall amino acid identity. IRP2 is ubiquitously expressed in rat tissues, the highest amounts present in skeletal muscle and heart. IRP2 is encoded by multiple mRNAs of 6.4, 4.0, and 3.7 kilobases. The 3'-untranslated region of rat IRP2 contains multiple polyadenylation signals, two of which could account for the 4.0-kb and 3.7-kb mRNAs. The 3.7-kb mRNA is increased in iron-depleted cells and occurs with a reciprocal decrease in the 6.4-kb transcript. These data suggest that the 3.7-kb mRNA is produced by alternative poly(A) site utilization in iron-depleted cells.


Assuntos
Regulação da Expressão Gênica , Ferro/fisiologia , RNA Mensageiro/análise , Proteínas de Ligação a RNA/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Carcinoma Hepatocelular/metabolismo , DNA Complementar/isolamento & purificação , Humanos , Proteína 1 Reguladora do Ferro , Proteína 2 Reguladora do Ferro , Proteínas Reguladoras de Ferro , Camundongos , Dados de Sequência Molecular , Ratos
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