Two cinnamoyloctopamine antioxidants from garlic skin attenuates oxidative stress and liver pathology in rats with non-alcoholic steatohepatitis.

Hepatic oxidative stress plays a key role in the development of non-alcoholic steatohepatitis (NASH), therefore, treatment approaches that address the antioxidant is helpful in the therapy of patients with NASH. N-trans-coumaroyloctopamine (1) and N-trans-feruloyloctopamine (2) were identified as the primary antioxidant constituents of garlic skin with high antioxidant activities. The aim of this study was to elucidate the protective effect and mechanism of the antioxidants on NASH in rats. The results provide morphological and molecular biological evidences for the protective role of the antioxidant 2 in ameliorating oxidative stress and hepatic apoptosis in experimental NASH for the first time. Mechanism study indicated that the antioxidant 2 significantly reduced the expression of COX-2 mRNA and protein by western blot, RT-PCR and immunohistochemical techniques.

[Study on the content of imperatorin and HPLC fingerprint of Baizhi collected from different areas].

OBJECTIVE: To compare the content of imperatorin and HPLC fingerprint of Baizhi collected from different areas. METHODS: The medicinal materials and seeds of Baizhi from 15 main habitats of 7 provinces and cities were collected, and the seeds were planted in the germplasm resource garden of Suining, Sichuan. The content of imperatorin and HPLC fingerprint were compared using the samples collected from different areas and germplasm resource garden collected and dried at the most suitable harvest date. RESULTS: The differences on the content of imperatorin and HPLC fingerprint were obvious. The content of imperatorin significantly increased and the HPLC fingerprint were trending to coincide when the herbs were planted in the same place. CONCLUSION: The environmental factors and the cultivation technique had great influences on the quality of Baizhi.