Apple polyphenols improve intestinal barrier function by enhancing antioxidant capacity and suppressing inflammation in weaning piglets.

This study aimed to examine the effects of apple polyphenols (APPs) on antioxidant capacity, immune and inflammatory response, and barrier function in weaning piglets. Results showed that APPs improved jejunal barrier function by increasing the villus height, villus height/crypt depth, the mRNA levels of occludin, mucin-1, and mucin-4 and up-regulating the protein expression of occluding (P < 0.05). As for antioxidant capacity, APPs increased the activities of total superoxide dismutase and glutathione peroxidase and total antioxidant capacity level in jejunum (P < 0.05). Besides, APPs up-regulated the protein expressions of NAD(P)H quinone dehydrogenase 1 (NQO1), heme oxygenase-1 (HO-1), and nuclear-related factor 2 (NRF2) and down-regulated the protein expression of kelch-like ECH-associated protein 1 (Keap1). As regard to immune and inflammatory response, APPs increased the immunoglobulin A content in serum and decreased the mRNA levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1ß, toll-like receptor 4 (TLR4), nuclear factor-κB (NF-κB), and IL-8 in jejunum (P < 0.05). Overall, dietary APPs supplementation improves the jejunal barrier function by enhancing antioxidant capacity and suppressing the mRNA expression related to inflammation, which may be related to the NRF2 signal and TLR4/NF-κB signal.

Effect of dietary dihydromyricetin supplementation on lipid metabolism, antioxidant capacity and skeletal muscle fiber type transformation in mice.

This study aimed to investigate the effect of dietary dihydromyricetin (DHM) supplementation on lipid metabolism, antioxidant capacity and muscle fiber type transformation. Twenty-four male Kunming mice were randomly allotted to either control (basal diet) or DHM diets (supplemented with 300 mg/kg DHM). Our data showed that DHM administration decreased the triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C) contents, and increased the catalase (CAT), total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px) activities in serum. In the liver, DHM decreased the TG and malondialdehyde (MDA) levels and increased the T-SOD and GSH-Px activities. For the tibialis anterior (TA) muscle, DHM increased the total antioxidant capacity (T-AOC) level and T-SOD activities. Western blotting and real-time quantitative PCR analysis showed that DHM increased the protein and mRNA levels of MyHC I and MyHC IIa and decreased the protein and mRNA levels of MyHC IIb in TA muscle, which may be achieved by activating the AMP-activated protein kinase (AMPK) signal. The mRNA levels of several regulatory factors related to mitochondrial function were up-regulated by DHM. In conclusion, dietary 300 mg/kg DHM supplementation improved lipid metabolism and antioxidant capacity and promoted the transformation of muscle fiber type from glycolysis to oxidation in mice.

Dietary dihydromyricetin supplementation enhances antioxidant capacity and improves lipid metabolism in finishing pigs.

Nowadays, chronic diseases have become a potential danger to human health and are highly concerning. Given that pigs are a suitable animal model for human nutrition and metabolism for its similar anatomical and physiological properties to those of humans, this study has used 24 castrated male Duroc × Landrace × Yorkshire (DLY) pigs as experimental subjects to explore the effects of dietary dihydromyricetin (DHM) supplementation on the antioxidant capacity and lipid metabolism. Results showed that dietary 300 and 500 mg DHM kg-1 diet supplementation increased the serum total superoxide dismutase (T-SOD) level, serum and liver reduced glutathione (GSH), muscle catalase (CAT) level and serum high-density lipoprotein cholesterol (HDL-C) level, and reduced the liver malondialdehyde (MDA) level and muscle triglyceride (TG) level in finishing pigs. Western blot analysis showed that dietary DHM supplementation activated the nuclear-related factor 2 (Nrf2) and AMP-activated protein kinase (AMPK)/acetyl-CoA carboxylase (ACC) signals. Real-time quantitative PCR analysis showed that dietary DHM supplementation upregulated the mRNA levels of lipolysis and fatty acid oxidation-related genes, and down-regulated the mRNA expression of lipogenesis-related genes in finishing pigs. Together, we provide evidence that dietary DHM supplementation improved the antioxidant capacity and lipid metabolism in finishing pigs.