RESUMO
To observe the therapeutic effect of treating pseudomyopia in children by auricular point sticking plus plucking manipulation. Two groups of auricular points were selected. There were Eye 1 (MA-L), Eye 2(MA-L), Liver (MA-SC 5) and Er Shenmen (MA-TF 1) in the first group and Central rim (MA-AT), Eye(MA-L 1), Superior Triangular Fossa (MA-TF) and Cervical Vertebrae (MA-AH 8)points. Then the cervical vertebrae were plucked and moved fore-and-aft circlewise. The result showed that among 45 affected eyes of the 35 cases, the total effective rate was 97.8%, which indicated the therapeutic effect was remarkable.
RESUMO
<p><b>OBJECTIVE</b>To investigate the effect of Zilongjin (ZLJ) on human androgen-dependent type of prostate cancer cell line LNCaP.</p><p><b>METHODS</b>MTT assay, flow cytometry and fluorescence microscopy were used to observe the effect of ZLJ in anti-proliferation, cell cycle arresting and apoptosis induction. RT-PCR was used to examine the effect of ZLJ on expressions of prostate marker gene (PSA), androgen receptor (AR), apoptosis related genes (bcl-2 and bax), and Western blot assay was used to detect the effect on protein expression of bcl-2 and bax.</p><p><b>RESULTS</b>ZLJ could cause apparent inhibition on proliferation, induce G0/G1 phase arresting and apoptosis in time- and dose-dependent manner on LNCaP cells. The concentration for inhibiting cell growth by 50% (IC50) in 72 hrs was 0.79 mg/ml. ZLJ could down-regulate the expression of PSA, AR, bcl-2 genes and lower bcl-2 protein expression, but showed ineffective on bax protein expression.</p><p><b>CONCLUSION</b>ZLJ displays its anti-tumor effects by way of inhibiting the cell proliferation, arresting the G0/G1 phase, inducing apoptosis, down-regulating PSA, AR, bcl-2 gene expression and lowering bcl-2 protein expressions.</p>