RESUMO
In this paper, the influence of the hepatoprotective drug Essentiale on the target (normal and regenerating liver) and the non-target (spleen and bone marrow) rat tissues was studied after whole body irradiation with the dose of 5.7 Gy gamma-radiation. The application of the drug 24 h before irradiation alleviated all the radiation induced changes of the histones and nucleic acids in the normal and regenerating liver tissue. In the non-target tissues only mild radioprotective effect was observed. The application of the preparation 30 min after irradiation was less effective than the application before irradiation. The repeated application of Essentiale after irradiation did not increase the beneficial effect of the previous preparation application.
Assuntos
Adjuvantes Imunológicos/farmacologia , Medula Óssea/efeitos da radiação , DNA/análise , Histonas/análise , Fígado/efeitos da radiação , Fosfatidilcolinas/farmacologia , RNA/análise , Protetores contra Radiação/farmacologia , Baço/efeitos da radiação , Animais , Medula Óssea/efeitos dos fármacos , Radioisótopos de Cobalto , Interpretação Estatística de Dados , Eletroforese em Gel de Poliacrilamida , Raios gama , Fígado/efeitos dos fármacos , Regeneração Hepática , Masculino , Doses de Radiação , Ratos , Ratos Wistar , Espectrofotometria , Baço/efeitos dos fármacosRESUMO
Radioprotective and therapeutical effect of silymarin (Flavobion) on development and repair of latent injury in rat liver was examined by its application during the continual gamma irradiation (dose rates 0.2 and 0.6 Gy/day) or after acute gamma irradiation (dose 6 Gy). Silymarin influence was evaluated on the basis of mitotic index and chromosomal aberration frequency in the liver regenerating after partial hepatectomy. We have found that silymarin application stimulates the process of liver regeneration in non-irradiated rats as well as in irradiated ones. Positive effect of silymarin (100 mg per kg p.o. ones per day) was manifested at both dose rates of continual irradiation with increase in mitotic activity and mitigation of chromosomal erration frequency in the regenerating liver in comparison with non-protected irradiated animals. Curative effect of silymarin (70 mg/kg p.o., twice per day) was shown especially after 14 days of its postradiation application.
Assuntos
Hepatopatias/prevenção & controle , Fígado/efeitos da radiação , Lesões Experimentais por Radiação/prevenção & controle , Protetores contra Radiação/uso terapêutico , Silimarina/uso terapêutico , Animais , Aberrações Cromossômicas , Relação Dose-Resposta à Radiação , Avaliação Pré-Clínica de Medicamentos , Raios gama , Fígado/ultraestrutura , Hepatopatias/tratamento farmacológico , Hepatopatias/etiologia , Masculino , Lesões Experimentais por Radiação/complicações , Lesões Experimentais por Radiação/tratamento farmacológico , Ratos , Ratos Wistar , Fatores de TempoRESUMO
The therapeutical effect of the hepatoprotective drug silymarin (Flavobion) was investigated in rats after total body gamma irradiation with a dose of 6 Gy. Silymarin (70 mg kg-1 p.o. by tube) was administered twice a day over 7 and 14 days after irradiation. At the end of therapy, the effect of silymarin was evaluated on the basis of quantitative changes of nucleic acids in the liver (regenerating after partial hepatectomy), spleen and bone marrow. It was found that the nucleic acid changes in irradiated rats were alleviated by the post-radiation application of silymarin in its target organ--the liver, but also in the spleen and bone marrow. We assume that the therapeutical effect of silymarin on radiation induced changes of nucleic acids in various tissues of rats is caused mainly by the activation of cellular metabolism including the metabolism of nucleic acids.