RESUMO
In two experiments adult rats (aged at least 6 months at the start of the procedure) received a diet enriched with added choline for a period of 10 weeks; control subjects were maintained on a standard diet during this time. All rats then underwent the spontaneous object recognition (SOR) procedure in which they were exposed to a pair of objects and then tested, after a retention interval, to a display with one object changed. Exploration of the changed object indicates retention and use of information acquired during the exposure phase. All subjects showed retention with a 24-h interval (Experiments 1 and 2) and when retested after a further 24â¯h (Experiment 1). But when tested for the first time after a 48-h interval (Experiment 2), control subjects showed no evidence of retention, exploring both objects equally, whereas those given the dietary supplement continued to show a preference for the changed object. This supports the conclusion that dietary choline supplementation can enhance performance on a task regarded as a test of declarative memory, and will do so even when the supplementations is given in adulthood.
Assuntos
Colina/administração & dosagem , Suplementos Nutricionais , Reconhecimento Psicológico , Animais , Masculino , Distribuição Aleatória , Ratos Wistar , Reconhecimento Psicológico/fisiologia , Fatores de TempoRESUMO
Waste stabilization ponds (WSPs), as part of municipal wastewater treatment strategies, can exhibit variability in performance due to climatic conditions. Under elevated temperature and strong solar radiation, algal blooms and subsequent high pH effluents have often been observed. In this study, four substrates (gravel, peat, organic mulch, and topsoil) were evaluated for their ability to attenuate high pH effluents from a WSP. Synthetic wastewater with pH > 9.5, and low organic and nutrient loadings, was used to mimic algal-induced high pH effluents in 72 L rectangular bench-scale superficial constructed wetland configuration reactors. Peat exhibited the highest attenuation ability, where the effluent pH decreased substantially from 10.3 to 7.7, primarily due to its high organic contents. Peat also removed 53.7% of the influent total phosphorus, which could effectively limit algal growth. No statistically significant differences were discovered among gravel, topsoil, and organic mulch in terms of pH attenuation. Topsoil and organic mulch both have a relatively high alkalinity, making them ideal to maintain consistent pH levels. However, naturally high chemical oxygen demand levels in organic mulch raised concerns in the leaching of these compounds into the treated wastewater, making it less appealing for systems with low organic loading.
Assuntos
Eliminação de Resíduos Líquidos/métodos , Águas Residuárias , Purificação da Água , Áreas Alagadas , Análise da Demanda Biológica de Oxigênio , Concentração de Íons de Hidrogênio , Fósforo/análise , Projetos Piloto , Lagoas , Água , Poluição da Água/prevenção & controleRESUMO
The traditional view of the medial temporal lobe (MTL) focuses on its role in episodic memory. However, some of the underlying functions of the MTL can be ascertained from its wider role in supporting spatial cognition in concert with parietal and prefrontal regions. The MTL is strongly implicated in the formation of enduring allocentric representations (e.g., O'Keefe, 1976; King et al., 2002; Ekstrom et al., 2003). According to our BBB model (Byrne et al., 2007), these representations must interact with head-centered and body-centered representations in posterior parietal cortex via a transformation circuit involving retrosplenial areas. Egocentric sensory representations in parietal areas can then cue the recall of allocentric spatial representations in long-term memory and, conversely, the products of retrieval in MTL can generate mental imagery within a parietal "window." Such imagery is necessarily egocentric and forms part of visuospatial working memory, in which it can be manipulated for the purpose of planning/imagining the future. Recent fMRI evidence (Lambrey et al., 2012; Zhang et al., 2012) supports the BBB model. To further test the model, we had participants learn the locations of objects in a virtual scene and tested their spatial memory under conditions that impose varying demands on the transformation circuit. We analyzed how brain activity correlated with accuracy in judging the direction of an object (1) from visuospatial working memory (we assume transient working memory due to the order of tasks and the absence of change in viewpoint, but long-term memory retrieval is also possible), (2) after a rotation of viewpoint, or (3) after a rotation and translation of viewpoint (judgment of relative direction). We found performance-related activity in both tasks requiring viewpoint rotation (ROT and JRD, i.e., conditions 2 and 3) in the core medial temporal to medial parietal circuit identified by the BBB model. These results are consistent with the predictions of the BBB model, and shed further light on the neural mechanisms underlying spatial memory, mental imagery and viewpoint transformations.
RESUMO
In two experiments adult rats were maintained on a diet enriched with added choline for 12 weeks prior to behavioral testing; control rats remained on the standard diet during this time. In Experiment 1 all rats received training in the Hall-Pearce negative transfer paradigm in which prior training with a conditioned stimulus (CS) paired with a small reinforcer retards further learning when the size of the reinforcer is increased. This effect, which has been attributed to a loss of associability by the CS, was obtained in control subjects but not in those given the supplement. Experiment 2 investigated the effect of prior nonreinforced exposure of the to-be-CS (latent inhibition). Such exposure retarded subsequent learning in control subjects, but latent inhibition was not obtained in those given the supplement. We conclude that the mechanism that reduces the attention paid to a stimulus that accurately predicts its consequences does not operate effectively after choline supplementation. These results are consistent with a role for the cholinergic system of the basal forebrain in modulation of attention.
Assuntos
Atenção/efeitos dos fármacos , Comportamento Animal/efeitos dos fármacos , Colina/farmacologia , Condicionamento Clássico/efeitos dos fármacos , Lipotrópicos/farmacologia , Animais , Colina/administração & dosagem , Inibição Psicológica , Lipotrópicos/administração & dosagem , Masculino , Testes Psicológicos , Ratos , Ratos WistarRESUMO
The neuronal mechanisms underlying the pathophysiology of bipolar disorder (BD) have not been fully characterized. The aim of this study was to compare metabolite levels in the hippocampus and the orbitofrontal cortex in a homogenous population of 12 euthymic patients with well-established BD and 12 age- and sex-matched healthy comparison subjects. Using a GE Signa, 3-Tesla scanner, we performed proton magnetic resonance spectroscopy (H-MRS) to examine levels of N-acetyl aspartate, glutamate and choline-containing compounds. Choline-containing compounds were significantly increased in the hippocampus and the orbitofrontal cortex in BD patients relative to control subjects. Significant elevations of glycerophosphocholine+phosphocholine (GPC+PCh) were measured in the hippocampus and the orbitofrontal cortex of patients. As choline is a marker of membrane phospholipid metabolism, the elevated choline in patients may indicate increased membrane breakdown in the brain regions examined. Abnormal neuronal loss within the hippocampus and orbitofrontal cortex further supports previous work suggesting that these regions are involved in the pathophysiology of BD.