Assuntos
Benzoatos/efeitos adversos , Hidrazinas/efeitos adversos , Isquemia/etiologia , Púrpura Trombocitopênica Idiopática/tratamento farmacológico , Pirazóis/efeitos adversos , Trombose/induzido quimicamente , Idoso , Biópsia , Clopidogrel/uso terapêutico , Angiografia por Tomografia Computadorizada , Tratamento Conservador , Feminino , Humanos , Oxigenoterapia Hiperbárica , Isquemia/patologia , Isquemia/terapia , Contagem de Plaquetas , Púrpura Trombocitopênica Idiopática/sangue , Pele/irrigação sanguínea , Pele/diagnóstico por imagem , Pele/patologia , Trombose/complicações , Trombose/diagnóstico , Trombose/tratamento farmacológico , Artérias da Tíbia/diagnóstico por imagem , Artérias da Tíbia/patologia , Dedos do Pé/irrigação sanguínea , Dedos do Pé/diagnóstico por imagem , Dedos do Pé/patologia , Resultado do Tratamento , Varfarina/uso terapêuticoRESUMO
Cysteine-based peroxidases, known as peroxiredoxins (Prx) or thioredoxin peroxidases (TPx), are important antioxidant enzymes that prevent oxidative damage caused by reactive oxygen species (ROS). In this study, we identified a novel mitochondrial 2-Cys Prx, BbTPx-2, from a bovine Babesia parasite, B. bovis. BbTPx-2 complementary DNA (cDNA) encodes a polypeptide of 254 amino acid residues. This protein has a mitochondrial targeting peptide at the N-terminus and two conserved cysteine residues of the typical 2-Cys Prx. By using a thiol mixed-function oxidation assay, the antioxidant activity of recombinant BbTPx-2 was revealed, and its antioxidant activity was comparable to that of a cytosolic 2-Cys Prx from B. bovis, BbTPx-1. Notably, we confirmed that BbTPx-2 was expressed in the mitochondrion of B. bovis merozoites. Taken together, the results suggest that the mitochondrial BbTPx-2 is an antioxidative enzyme for scavenging ROS in B. bovis.
Assuntos
Antioxidantes/metabolismo , Babesia bovis/enzimologia , Mitocôndrias/enzimologia , Peroxirredoxinas/metabolismo , Sequência de Aminoácidos , Animais , Babesia bovis/metabolismo , Sequência de Bases , Bovinos , Cisteína/química , DNA Complementar/genética , Mitocôndrias/genética , Oxirredução , Peroxirredoxinas/genética , Espécies Reativas de Oxigênio/metabolismo , Alinhamento de SequênciaRESUMO
A 3.5 yr old spayed female Staffordshire terrier weighing 25.5 kg was presented with a 7 wk history of bilateral plantigrade stance in the pelvic limbs directly following an ovariohysterectomy procedure. Upon presentation, the dog had bilateral atrophy of the distal pelvic limb muscles, enlarged popliteal lymph nodes, and ulcerative wounds on the dorsa of her rear paws. Orthopedic examination revealed intact calcaneal tendons bilaterally and neurologic examination localized the lesion to the distal sciatic nerve. A diagnosis of compressive and stretch neuropathy was made affecting the distal sciatic nerve branches. Physical therapy modalities included neuromuscular electrical stimulation, ultrasound, and low-level laser therapy. Other therapeutic modalities included the use of orthotics and progressive wound care. The dog had increased muscle mass, return of segmental reflexes, return of nociception, and the ability to walk on pelvic limbs with higher carriage of the hock 15 mo following presentation. The use of custom orthotics greatly increased the quality of life and other physical therapy modalities may have improved the prognosis in this dog with severe bilateral plantigrade stance due to neuropathy.