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1.
Fitoterapia ; 173: 105806, 2024 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-38181893

RESUMO

Actinomadura sp., which is usually found in muddy habitats, produces various secondary metabolites with biological activities. In this study, five new compounds named formosensin A (1), formosensin B (2), oxanthroquinone-3-O-α-d-mannose (8), oxanthromicin A (9), and oxanthromicin B (10) were isolated from the culture of Actinomadura sp. together with five known compounds (3-7). Their structures were elucidated by extensive spectroscopic methods including NMR and MS. In particular, the absolute configurations of compounds 1 and 2 were determined using computational methods. Moreover, compounds 1-2 and 8-10 were screened for cytotoxic activity using a panel of human tumor cell lines. Compound 9 induced significant cytotoxicity in five human tumor cell lines (HL-60, A-549, SMMC-7721, MCF-7, and SW480) with IC50 values of 8.7, 17.5, 15.0, 17.8, and 14.6 µM, respectively. These findings suggested that compound 9 could provide therapeutic benefits in the treatment of tumor-related diseases.


Assuntos
Actinomadura , Antineoplásicos , Humanos , Estrutura Molecular , Antineoplásicos/farmacologia , Linhagem Celular Tumoral , Antraquinonas
2.
Fitoterapia ; 152: 104875, 2021 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-33675886

RESUMO

One new limonoid, named 19-hydroxy methyl isoobacunoate diosphenol (1); one new degraded limonoid, named 9α-methoxyl dictamdiol (9); two new quinolone alkaloids, 1-methyl-3-[(7E,9E,12Z)-7,9,12-pentadecadienyl]-4(1H)-quinolone (11) and 1-methyl-3-[(7E,9E,11E)-7,9,11-pentadecadienyl]-4(1H)-quinolone (12), along with eight known compounds, evodol (2), 7ß-acetoxy-5-epilimonin (3), rutaevine (4), 6ß-acetoxy-5-epilimonin (5), limonin (6), obacunone (7), clauemargine L (8), hiiranlactone E (10) were isolated from the fruits of Evodia rutaecarpa (Juss.) Benth.. Structures of the four new compounds were elucidated on the basis of extensive spectroscopic techniques, including 1D and 2D NMR techniques. Compounds 3, 5, 9, 11 and 12 showed obviously cytotoxic activity against six human tumor lines, while compounds 11, 12 displayed anti-platelet aggregation induced by ADP at 50 µM and 100 µM.


Assuntos
Alcaloides/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Evodia/química , Limoninas/farmacologia , Quinolonas/farmacologia , Alcaloides/isolamento & purificação , Antineoplásicos Fitogênicos/isolamento & purificação , Plaquetas/efeitos dos fármacos , Linhagem Celular Tumoral , China , Frutas/química , Humanos , Limoninas/isolamento & purificação , Estrutura Molecular , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Agregação Plaquetária/efeitos dos fármacos , Quinolonas/isolamento & purificação
3.
Fitoterapia ; 120: 142-145, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28614697

RESUMO

Three new bisditerpenoid alkaloids, navicularines A-C (1-3), and three known ones (4-6), were isolated from the ground parts of Aconitum naviculare. Their structures were elucidated by spectroscopic methods. All the new compounds were tested against five cell lines (HL-60, SMMC-7721, A-549, MCF-7, SW480). It was found that navicularine B exhibited certain cytotoxic activities in vitro, with IC50 values of 13.50, 18.52, 17.22, 11.18, and 16.36µM, respectively.


Assuntos
Aconitum/química , Alcaloides/farmacologia , Antineoplásicos Fitogênicos/farmacologia , Diterpenos/farmacologia , Alcaloides/isolamento & purificação , Antineoplásicos Fitogênicos/isolamento & purificação , Linhagem Celular Tumoral , Diterpenos/isolamento & purificação , Humanos , Estrutura Molecular , Raízes de Plantas/química
4.
Yao Xue Xue Bao ; 51(4): 613-5, 2016 04.
Artigo em Chinês | MEDLINE | ID: mdl-29860745

RESUMO

To study the constituents of Karelinia caspia(Pall.) Less, three phenylpropanoid derivatives and other compounds were isolated by silica gel, RP-18 chromatographic methods. Compound 1 was a new phenylpropanoid glycoside named as kareline A. Their structures were determined by spectroscopic analysis, including 1D- and 2D-NMR and mass spectrometry.


Assuntos
Asteraceae/química , Glicosídeos/isolamento & purificação , Extratos Vegetais/química , Propanóis/isolamento & purificação , Glicosídeos/química , Estrutura Molecular , Propanóis/química
5.
Trials ; 16: 378, 2015 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-26303741

RESUMO

BACKGROUND: Although many patients with facial paralysis have obtained benefits or completely recovered after acupuncture or electroacupuncture therapy, it is still difficult to list intuitive evidence besides evaluation using neurological function scales and a few electrophysiologic data. Hence, the aim of this study is to use more intuitive and reliable detection techniques such as facial nerve magnetic resonance imaging (MRI), nerve electromyography, and F waves to observe changes in the anatomic morphology of facial nerves and nerve conduction before and after applying acupuncture or electroacupuncture, and to verify their effectiveness by combining neurological function scales. METHODS/DESIGN: A total of 132 patients with Bell's palsy (grades III and IV in the House-Brackmann [HB] Facial Nerve Grading System) will be randomly divided into electroacupuncture, manual acupuncture, non-acupuncture, and medicine control groups. All the patients will be given electroacupuncture treatment after the acute period, except for patients in the medicine control group. The acupuncture or electroacupuncture treatments will be performed every 2 days until the patients recover or withdraw from the study. The primary outcome is analysis based on facial nerve functional scales (HB scale and Sunnybrook facial grading system), and the secondary outcome is analysis based on MRI, nerve electromyography and F-wave detection. All the patients will undergo MRI within 3 days after Bell's palsy onset for observation of the signal intensity and facial nerve swelling of the unaffected and affected sides. They will also undergo facial nerve electromyography and F-wave detection within 1 week after onset of Bell's palsy. Nerve function will be evaluated using the HB scale and Sunnybrook facial grading system at each hospital visit for treatment until the end of the study. The MRI, nerve electromyography, and F-wave detection will be performed again at 1 month after the onset of Bell's palsy. TRIAL REGISTRATION: Chinese Clinical Trials Register identifier: ChiCTR-IPR-14005730. Registered on 23 December 2014.


Assuntos
Terapia por Acupuntura , Paralisia de Bell/terapia , Eletroacupuntura , Nervo Facial/fisiopatologia , Terapia por Acupuntura/efeitos adversos , Doença Aguda , Adolescente , Adulto , Paralisia de Bell/diagnóstico , Paralisia de Bell/fisiopatologia , China , Protocolos Clínicos , Eletroacupuntura/efeitos adversos , Eletromiografia , Feminino , Humanos , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade , Condução Nervosa , Exame Neurológico , Recuperação de Função Fisiológica , Projetos de Pesquisa , Índice de Gravidade de Doença , Fatores de Tempo , Resultado do Tratamento , Adulto Jovem
6.
Fitoterapia ; 104: 50-4, 2015 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-25987321

RESUMO

Three new brasilane-type sesquiterpenoids, brasilanes A-C (1-3), together with two new alkane derivatives, colisiderin A (4) and 7(E),9(E)-undecandiene-2,4,5-triol (5), were isolated from cultures of the basidiomycete Coltricia sideroides. Their structures were elucidated by NMR and MS data analyses. The absolute configuration of 4 was determined by TDDFT ECD calculations while brasilane-type sesquiterpenoids were isolated from cultures of mushroom for the first time. Compounds 2 and 4 showed weak cytotoxicities against HL-60 and SW480, respectively.


Assuntos
Basidiomycota/química , Sesquiterpenos/química , Agaricales/química , Antineoplásicos/química , Antineoplásicos/isolamento & purificação , Linhagem Celular Tumoral/efeitos dos fármacos , Células HL-60/efeitos dos fármacos , Humanos , Estrutura Molecular , Sesquiterpenos/isolamento & purificação
7.
Fitoterapia ; 102: 1-6, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25665943

RESUMO

Seven new drimane-type sesquiterpenoids, sulphureuines B-H (1-7), together with four known compounds (8-11), were obtained from cultures of mushroom Laetiporus sulphureus. All of these compounds were tested for their cytotoxicities against five human cancer cell lines (HL-60, SMMC-721, A-549, MCF-7, SW-480), compound 10 showed potent cytotoxic activity against HL-60, SMMC-721, A-549, SW-480, with IC50 values of 37.5, 14.8, 15.6, and 36.1µM, respectively.


Assuntos
Antineoplásicos/química , Polyporales/química , Sesquiterpenos/química , Agaricales/química , Antineoplásicos/isolamento & purificação , Linhagem Celular Tumoral , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Estrutura Molecular , Sesquiterpenos Policíclicos , Sesquiterpenos/isolamento & purificação
8.
Chem Biodivers ; 8(12): 2270-6, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22162164

RESUMO

Two coriamyrtin-type sesquiterpenes, fengfangin A (1) and tutin (2), and six diarylheptanoids, namely alnusone (3), centrolobol (4), muricarpone B (5), 1-(3,4-dihydroxyphenyl)-7-(4-hydroxyphenyl)heptan-3-one (6), (3S)-1-(3,4-dihydroxyphenyl)-7-(4-hydroxyphenyl)heptan-3-ol (7), and (3S)-1-(4-hydroxyphenyl)-7-(3,4-dihydroxyphenyl)heptan-3-ol (8), were isolated from the 95% EtOH extract of nidus vespae, the nest of Polistes species. Their structures were identified by spectroscopic methods. Compounds 1 and 8 are new products. The absolute configuration of 1 was determined by single-crystal X-ray diffraction analysis using Flack parameter. The biological tests showed that compounds 5, 6, and 8 could inhibit nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells with IC(50) values in the range of 13-17 µM, whereas the sesquiterpenes were inactive in this assay (>25 µM). In addition, the ecological significance of the presence of neurotoxic sesquiterpene lactones in nidus vespae is briefly discussed.


Assuntos
Anti-Inflamatórios não Esteroides/isolamento & purificação , Diarileptanoides/isolamento & purificação , Óxido Nítrico/efeitos adversos , Sesquiterpenos/isolamento & purificação , Vespas/química , Animais , Anti-Inflamatórios não Esteroides/química , Anti-Inflamatórios não Esteroides/farmacologia , Linhagem Celular , Diarileptanoides/química , Diarileptanoides/farmacologia , Macrófagos/efeitos dos fármacos , Macrófagos/metabolismo , Medicina Tradicional Chinesa , Camundongos , Modelos Moleculares , Estrutura Molecular , Sesquiterpenos/química , Sesquiterpenos/farmacologia , Vespas/crescimento & desenvolvimento
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