RESUMO
Soybean meal is one of the most promising alternatives to replace fishmeal in the aquaculture industry. However, its ingestion triggers an intestinal inflammatory process that compromises fish health and nutrition. Therefore, finding strategies that reduce the deleterious effects of a soy protein-based diet are relevant. In this work we analyzed the effects of an aloe vera (Aloe barbadensis miller, AV) extract on intestinal inflammation and innate immunity of zebrafish by adding it to the water and by supplementing it in a soybean meal-based diet. To search for potential immunomodulatory effects of AV, we tested its effectiveness in two inflammation assays and compared fish fed with either fishmeal or soybean meal-based feed supplemented with AV. Our results show a strong anti-inflammatory effect of AV. Furthermore, while soy-based meal strongly induces the expression of inflammation markers, supplementation with AV reverted this effect. Finally, we show that fish fed with a soy meal diet are highly susceptible to bacterial infection, but that this condition is significantly reduced when the soy meal is supplemented with AV. Our results suggest that AV is a good candidate to be incorporated as an additive in farmed fish diets to facilitate the replacement of fishmeal by soybean meal, maintaining intestinal health.
Assuntos
Aloe/química , Anti-Inflamatórios/uso terapêutico , Inflamação/terapia , Intestinos/imunologia , Extratos Vegetais/uso terapêutico , Proteínas de Soja/efeitos adversos , Peixe-Zebra/imunologia , Ração Animal , Animais , Aquicultura , Suplementos Nutricionais/análise , Intestinos/efeitos dos fármacos , Proteínas de Soja/administração & dosagemRESUMO
Assessing the environmental impact of a soil-topped landfill requires an accurate ecotoxicological diagnosis. This paper describes various diagnostic protocols for this purpose and their application to a real case: the urban solid waste (USW) municipal landfill of Getafe (Madrid, Spain). After their initial sealing with soil from the surroundings about 20 years ago, most USW landfills in the autonomous community of Madrid have continued to receive waste. This has hindered precise assessment of their impact on their environment and affected ecosystems. The procedure proposed here overcomes this problem by assessing the situation in edaphic, aquatic and ecological terms. The present study focused on the most influential soil variables (viz. salinity due largely to the presence of anions, and heavy metals and organic compounds). These variables were also determined in surface waters of the wetland most strongly affected by leachates running down landfill slopes. Determinations included the characterization of plant communities and microbial biodiversity. The study was supplemented with a bioassay under controlled conditions in pots containing soil contaminated with variable concentrations of Zn (as ZnCl(2)) intended to assess ecochemical actions in a population of Bromus rubens, which grows profusely in the landfill.
Assuntos
Ecotoxicologia/métodos , Eliminação de Resíduos , Poluentes do Solo/análise , Solo , Biodiversidade , Biota , Bromus , Metais Pesados/análise , Compostos Orgânicos/análise , Plantas , Microbiologia do Solo , Espanha , Áreas Alagadas , Zinco/análiseRESUMO
Diverticulitis of the rectum is a rare condition. This report covers patient history, diagnosis, and treatment involved in such a case. The patient presented with a history of rectal pain and muscle spasm of six months' duration. After several available examinations had been completed, i.e., digital examination, sigmoidoscopy, and barium-enema examination, the diagnosis of a rectal diverticulum was made. Initially, conservative treatment, including high-fiber diet and sitz baths, proved effective. Approximately nine months later, the patient developed severe rectal pain, unrelieved by previously effective measures. After the above-described examinations had been repeated, the rectal wall was found to be ulcerated and inflamed, and a diagnosis of diverticulitis of the rectum was made. Antibiotic therapy and evacuation of the 3- to 4-cm mass under anesthesia resulted in subsidence of symptoms and resolution of the mass. Segmental resection will be considered if the diverticulum becomes infected again.