Biosynthesis, characterization, bactericidal and sporicidal activity of silver nanoparticles using the leaves extract of Litchi chinensis.

Biosynthesis of silver nanoparticles (AgNPs) using plant extracts has become a promising alternative to the conventional chemical synthesis approach. In this study, cost-effective synthesis of AgNPs was attempted using leaves extract of Litchi chinensis. Bio-reduction reaction for the synthesis of NPs was checked by confirming the presence of AgNPs in solution by UV-vis spectrophotometry and with further characterization by fourier-transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). Surface plasmon resonance (SPR) band showed absorption peak at 422 nm indicating the formation of AgNPs, and FTIR spectra confirmed the presence of biological molecules involved in AgNPs synthesis. TEM analysis revealed the spherical shape of AgNPs with particle size distribution in a range of 5-15 nm. Further, the biosynthesized AgNPs showed significant bactericidal and sporicidal activity against model spore former Bacillus subtilis. AgNPs at concentrations ranging from 25 to 100 µg/mL showed bactericidal activity with inhibition zone ranging from 4-19 mm and sporicidal activity at 100-200 µg/mL in a range of 4.46-61.6% with an exposure time of 2-8 h. These findings exhibit distinctive potential of biogenic AgNPs for their efficient use in developing novel bactericidal and sporicidal agent against spore forming bacilli.

ß-sitosterol in different parts of Saraca asoca and herbal drug ashokarista: Quali-quantitative analysis by liquid chromatography-mass spectrometry.

ß-sitosterol is an important component in food and herbal products and beneficial in hyperlipidemia. Its higher concentrations in serum may lead to coronary artery disease in case of sitosterolemia. Therefore, it is essential to determine the quantity of ß-sitosterol in food and herbal drugs. Saraca asoca and its preparations have been widely used by traditional healers are also a source of ß-sitosterol. In the present study, quantitative estimation of ß-sitosterol present in hot and cold water extracts of bark, regenerated bark, leaves and flowers of the S. asoca and Ashokarista drugs were carried out first time using high performance liquid chromatography coupled (HPLC) with quadrupole time-of-flight mass spectrometry. Different concentrations of ß-sitosterol and crude extracts were estimated by HPLC and targeted mass spectrometry. Standard curve for ß-sitosterol was prepared from the intensities of transitions (397.50 → 147.0987 m/z) having regression coefficient (r (2)) 0.9952. Out of eight extracts and two drugs used in the study bark water, leaves water and leaves hot water extracts were found to have a considerable quantity of ß-sitosterol, i.e. 170, 123.5 and 19.3 ng/mL, respectively. The results showed significant differences in the distribution of ß-sitosterol among different organs of S. asoca and drugs prepared from its bark. HPLC/electrospray ionizationmass spectroscopy method is accurate, reproducible and requires less specimen, sample preparation and analysis time over HPLC assay. This type of approaches could be helpful for the quality control of herbal medicines and provides necessary information for the rational utilization of plant resources.