[Effects of cultivating Coptis chinensis and Paris polyphylla on soil microarthropod communities.] / 种植黄连和重楼对小型土壤节肢动物群落的影响.

In recent years, the area of herbal medicine planting is rapidly increasing. The effects of planting herbal medicines on soil invertebrate communities are still unclear. To reveal the effects of planting different herbal medicines on the soil microarthropod communities, soil microarthropods in two fields of planting Coptis chinensis and Paris polyphylla for 3-year and 5-year, respectively, were investigated in Pengzhou, Chengdu in July 2020. A total of 526 individuals of soil microarthropods were recorded and classified into 4 classes, 17 orders, 69 families, and 98 genera or taxonomic groups. The communities were dominated by Isotoma, Piatynothrus, Folsomia, and Paranura. The community structure of soil microarthropods differed obviously among the two herbal medicine fields, with the main influencing taxonomic groups of Proisotoma, Ocesobates and Epicridae. The total taxonomic group richness of soil microarthropods were richer in C. chinensis field than P. polyphylla field. There was no significant difference in the abundance and diversity index between the two fields. With the increases of cultivating years, the abundance of soil microarthropods in C. chinensis field declined significantly, and Shannon index increased significantly in P. polyphylla field. The redundancy analysis showed that the community structure of soil microarthropods was mainly affected by soil available N, pH, total K, and available K. It suggested that the effects of cultivating herbal medicines on soil microarthropod communities differed between herbal medicine species. Therefore, we recommended to intercrop C. chinensis and P. polyphylla for maintaining the stability of soil microarthropod diversity and promoting ecosystem function.

Resveratrol and Celastrol Loaded Collagen Dental Implants Regulate Periodontal Ligament Fibroblast Growth and Osteoclastogenesis of Bone Marrow Macrophages.

Collagen is widely used for dental therapy in several ways such as films, 3D matrix, and composites, besides traditional Chinese medicine (TCM), has been used in tissue regeneration and wound healing application for centuries. Hence, the present study was targeted for the first time to fabricate collagen film with TCM such as resveratrol and celastrol in order to investigate the human periodontal ligament fibroblasts (HPLF) growth and bone marrow macrophages (BMM) derived osteoclastogenesis. Further, the physicochemical, mechanical and biological activities of collagen-TCM films crosslinked by glycerol and EDC-NHS (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide-N-hydroxysulfosuccinimide) were investigated. Collagen film characterization was significantly regulated by the nature of plasticizers like hydrophobic and degree of polarity. Interestingly, the collagen film's denaturation temperature was increased by EDC-NHS than glycerol. FT-IR data confirmed the functional group changes due to chemical interaction of collagen with TCM. Morphological changes of HPLF cells cultured in control and collagen films were observed by SEM. Importantly, the addition of resveratrol upregulated the proliferation of HPLF cells, while osteoclastogenesis of BMM cells treated with mCSF-RANKL was significantly downregulated by celastrol. Accordingly, the collagen-TCM film could be an interesting material for dental regeneration, and especially it is a therapeutic target to restrain the elevated bone resorption during osteoporosis.

Effect of steam explosion on phenolic compounds and antioxidant capacity in adzuki beans.

BACKGROUND: Steam explosion is increasingly being used in the food processing industry as an efficient pretreatment technology. It is currently being used to pretreat adzuki beans at a pressure of 0.25-1.0 Mpa for 30 s and 90 s. In this study, the total polyphenol (TP) content in adzuki beans, including free polyphenols (FP) and bound polyphenols (BP), and their antioxidant activity, were determined after steam explosion treatment. RESULTS: The results showed that steam explosion can form large cavities and intercellular spaces, which aid the release of polyphenols. After steam explosion, the FP, BP, and TP content increased. The antioxidant capacity of FP and BP also increased, which demonstrated that there was a positive correlation between the polyphenol content and antioxidant capacity. Compounds of FP and BP were further identified by high-performance liquid chromatography (HPLC). Protocatechin was the main ingredient in FP and BP, and protocatechin was higher in FP. Isoquercetin only exists in FP, and caffeic acid only in BP. After steam explosion, an increase in the protocatechin, catechin, and epicatechin content was detected in FP and BP. The phenolic compound and antioxidant capacity yield was increased at a pressure of 0.25-0.75 Mpa, however it decreased at 1.0 Mpa. A pressure of 0.75 Mpa for 90 s is the optimal condition for polyphenol separation in adzuki beans. CONCLUSION: A proper and reasonable steam explosion can effectively increase the release of phenolics and enhance the antioxidant capacity in adzuki beans. © 2020 Society of Chemical Industry.