RESUMO
OBJECTIVE: To study the chemical constituents in roots of Caragana sinica. METHOD: The constituents were isolated by silica gel column chromatography, and their structures were identified by spectroscopic methods. RESULT: Seven compounds were identified as (+) - stenophyllol B (1), 4-hydroxybenzoic acid (2), odoratin (3), resveratrol (4), cararosinol A (5), leachinol C (6), carasinaurone (7) respectively. CONCLUSION: Compound 1 was a new compound which was the enantiomer of stenophyllol B. Compounds 2-6 were obtained from the plant for the first time.
Assuntos
Caragana/química , Medicamentos de Ervas Chinesas/química , Raízes de Plantas/química , Cromatografia em Gel , Medicamentos de Ervas Chinesas/análise , Estrutura Molecular , Parabenos/análise , Parabenos/química , Resveratrol , Estilbenos/análise , Estilbenos/químicaRESUMO
This study was intended to look for anti-HIV chemical constituents of aerial parts of Caragana rosea Turcz. Column chromatographic technique was used for the isolation and purification of constituents of Caragana rosea under the guide of anti-HIV assay. The structures were established on the basis of physical and chemical properties and spectroscopic data. Five compounds were obtained from the EtOAc fraction of aerial parts of Caragana rosea and identified as myricetin (1), mearnsetin (2), p-hydroxy cinnamic acid (3), cararosinol A (4) and cararosinol B (5). At the same time, one possible transformation route between cararosinol B and kobophenol A, another resveratrol tetramer isolated from this plant previously, was proposed. Compounds 4, 5 are new resveratrol tetramers, compounds 1 -3 were isolated from this plant for the first time. All compounds showed no activities in an in vitro assay against HIV-1.
Assuntos
Fármacos Anti-HIV/química , Benzofuranos/isolamento & purificação , Caragana/química , Componentes Aéreos da Planta/química , Plantas Medicinais/química , Estilbenos/isolamento & purificação , Fármacos Anti-HIV/isolamento & purificação , Fármacos Anti-HIV/farmacologia , Benzofuranos/química , Benzofuranos/farmacologia , Ácidos Cumáricos/química , Ácidos Cumáricos/isolamento & purificação , Ácidos Cumáricos/farmacologia , Flavonoides/química , Flavonoides/isolamento & purificação , Flavonoides/farmacologia , HIV-1/efeitos dos fármacos , Estrutura Molecular , Propionatos , Estilbenos/química , Estilbenos/farmacologiaRESUMO
The X-ray single-crystal structure of natural salidroside (=2-(4-hydroxyphenyl)ethyl beta-D-glucopyranoside; 1), isolated from Cistanche deserticola, is reported for the first time, as well as its absolute configuration. The radical-scavenging activity of 1 towards the superoxide radical anion (O*2-) was determined experimentally by chemiluminescence measurements of the pyrogallol-luminol system, and compared to that of the corresponding aglycone, i.e., tyrosol (=4-(2-hydroxyethyl)phenol; 2).
Assuntos
Antioxidantes/química , Glucosídeos/química , Glicosídeos/química , Fenóis/química , Antioxidantes/isolamento & purificação , Antioxidantes/farmacologia , Cistanche , Cristalografia por Raios X , Sequestradores de Radicais Livres/química , Sequestradores de Radicais Livres/isolamento & purificação , Sequestradores de Radicais Livres/farmacologia , Glucosídeos/isolamento & purificação , Glucosídeos/farmacologia , Glicosídeos/isolamento & purificação , Glicosídeos/farmacologia , Fenóis/isolamento & purificação , Fenóis/farmacologia , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Caules de PlantaRESUMO
The objectives of this research were to determine simultaneously the contents of two stilbene tetramers, carasinol B (1) and kobophenol A (2), and one stilbene trimer, (+)-alpha-viniferin (3), in Jin Que-gen in different regions. A HPLC method has been developed for efficiently quantifying the three analytes in the plant. Using this method, different samples of Jin Que-gen were evaluated. The results showed that contents of the three analytes varied significantly among different samples, and the contents of the three analytes in commercial Jin Que-gen were markedly lower than those in the plants collected directly from growing regions. And for the three analytes, the longer the cultivation time, the higher the contents.
Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/química , Extratos Vegetais/química , Plantas Medicinais/química , Estilbenos/análise , Benzofuranos/análise , Medicamentos de Ervas Chinesas/análise , Extratos Vegetais/análiseRESUMO
OBJECTIVE: To study the chemical constituents from Caragana intermedia. METHODS: The compounds were separated by chromatography methods. Their structures were identified by spectral analysis. RESULTS: Eight compounds were isolated and identified as 7,5'-dihydroxy-3 '-methoxyisoflavone-7-O-beta-D-glucoside (1), isorhamnetin 7-O-alpha-L-rhamnoside (2), 3, 4-dihydroxybenzoic acid (3), N-trans-caffeoyltyramine (4), D-3-O-methyl-inositol (5),7alpha-hydroxy-beta-sitosterol (6),7beta-hydroxy-beta-sitosterol (7) and stearic acid (8). CONCLUSION: All these compounds were obtained from this plant for the first time.
Assuntos
Caragana/química , Flavonoides/isolamento & purificação , Glicosídeos/isolamento & purificação , Plantas Medicinais/química , Flavonoides/química , Glicosídeos/química , Sitosteroides/química , Sitosteroides/isolamento & purificação , Ácidos Esteáricos/isolamento & purificaçãoRESUMO
The objectives of this research were to determine simultaneously the contents of two stilbene tetramers, carasinol B (1) and kobophenol A (2), and one stilbene trimer, (+)-alpha-viniferin (3), in the roots, tubers, and leaves of Caragana sinica in various seasons. A HPLC method has been developed for efficiently quantifying the three analytes in the plant. Using this method, different samples of Caragana sinica were evaluated. The results showed that the contents of 1, 2, and 3 in the roots were much higher than those in the tubers, and the contents of stilbene tetramers were maximal in winter while the contents of the stilbene trimer were maximal in summer. Compounds 1, 2, and 3 could not be detected in the flowers of Caragana sinica in our detection ranges.
Assuntos
Benzofuranos/química , Caragana/química , Estilbenos/química , Benzofuranos/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Extratos Vegetais/química , Folhas de Planta/química , Raízes de Plantas/química , Padrões de Referência , Reprodutibilidade dos Testes , Estações do Ano , Solventes , Estilbenos/isolamento & purificaçãoRESUMO
Natural products derived from plants provide a rich source for development of new anticancer drugs. Recent studies suggest that modulation of subcellular localization of retinoid X receptor-alpha (RXRalpha) represents a potential approach for inducing cancer cell apoptosis. In this study, we screened a herbal library for inducing translocation of RXRalpha from the nucleus to the cytoplasm. Our results revealed that the extract of Hypericum sampsonii, a member of the genus Hypericum, had remarkable effect on RXRalpha subcellular localization in various cancer cells. Treatment of NIH-H460 human lung cancer cells with H. sampsonii extract resulted in relocalization of RXRalpha from the nucleus to the cytoplasm. Cytoplasmic RXRalpha induced by H. sampsonii was associated with mitochondria, accompanied with cytochrome c release and apoptosis. H. sampsonii extract effectively inhibited the growth of various cancer cell lines, including NIH-H460 lung cancer, MGC-803 stomach cancer and SMMC7721 liver cancer cells. The growth inhibitory effect of H. sampsonii extract depended on levels of RXRalpha, as it failed to inhibit the growth of CV-1 cells lacking detectable RXRalpha, whereas transfection of RXRalpha into CV-1 cells restored its apoptotic response to H. sampsonii. Furthermore, the apoptotic effect of H. sampsonii was significantly enhanced when RXRalpha was overexpressed in NIH-H460 cells. Together, our results demonstrate that H. sampsonii contains ingredient(s) that induce apoptosis of cancer cells by modulating subcellular localization of RXRalpha.
Assuntos
Apoptose , Núcleo Celular/metabolismo , Hypericum , Receptor X Retinoide alfa/metabolismo , Transporte Ativo do Núcleo Celular , Linhagem Celular Tumoral , Humanos , Transporte Proteico , Transdução de Sinais , Ativação TranscricionalRESUMO
This research aims to study the metabolism and pharmacokinetics of phytoestrogen kobophenol A (1), the main active compound of Caragana sinica (Buc'hoz) Rehd. (Fabaceae), in rats. Metabolites of 1 in rats' feces were isolated and purified by multi-chromatograph techniques; three new metabolites of 1, named koboquinone A (M1), koboquinone B (M2) and koboquinone C (M3), were isolated, purified from rats' feces after they being orally administered with 1. Structure identification of the metabolites was fulfilled by spectroscopic analysis. M1 and M2 are structurally different to those natural occurring stilbene tetramers, which also have para-quinone structure. M1 also showed the activity of stimulating the proliferation of cultured osteoblasts. The pharmacokinetics of 1 in rats could be described by a two-compartmental model (P<0.05). The half-life was 0.68 h for i.v. administration and 5.78 h for oral administration. The oral bioavailability of 1 was calculated to be 2.0%; rats tissue distribution experiments show that 1 was prominently concentrated in livers. Both of the low oral bioavailability and the rapid reduction of 1 in blood indicated a suitable formulation is needed while it is developed as a new drug.
Assuntos
Caragana/química , Fitoestrógenos/farmacocinética , Estilbenos/farmacocinética , Animais , Masculino , Ratos , Ratos Sprague-Dawley , Distribuição TecidualRESUMO
Bioassay-guided fractionation of the ethanol extract of the aerial parts of Caragana rosea Turcz. led to the isolation of two new (cararosinol C and cararosinol D) and four known [scirpusin A (1), cis-scirpusin A (2), maackin (3), scirpusin B (4)] stilbene dimers. This is the first identification of these compounds from this plant and the first time that compound 2 has been isolated as a natural compound. Their structures were established mainly by spectral means. An in vitro assay against HIV-1 (IIIB), demonstrated compounds 1 and 4 to be active against HIV, with EC50 values of 10 microg/mL and 7 microg/mL respectively.
Assuntos
Fármacos Anti-HIV/farmacologia , Caragana , HIV-1/efeitos dos fármacos , Fitoterapia , Extratos Vegetais/farmacologia , Estilbenos/farmacologia , Fármacos Anti-HIV/administração & dosagem , Fármacos Anti-HIV/uso terapêutico , Humanos , Concentração Inibidora 50 , Componentes Aéreos da Planta , Extratos Vegetais/administração & dosagem , Extratos Vegetais/uso terapêutico , Estilbenos/administração & dosagem , Estilbenos/uso terapêuticoRESUMO
Four new eudesmanes, namely, 4(15)-eudesmene-1beta,7alpha-diol (1), 4(15)-eudesmene-1beta,7beta-diol (2), 7-trinoreudesma-4(15),8-dien-1beta-ol-7-one (3), and eudesma-4(15),7-dien-1beta-ol (4), as well as three known compounds, 5-epi-eudesma-4(15)-ene-1beta,6beta-diol (5), 4(15)-eudesmene-1beta,6alpha-diol (6), and 4(15)-eudesmene-1beta,5alpha-diol (7), were isolated from the aerial part of Caragana intermedia. The structures were elucidated by spectroscopic and spectrometric analyses including 1D, 2D NMR, HRMS, and IR. The structures of compounds 1, 5, 6, and 7 were confirmed by X-ray crystallographic analysis. Compound 7 showed glucose consumption activity with an IC value of 10.7 microg/mL in a C2C12 muscle cell assay. The MIC value of this compound (100 mg/kg) in a db/db mice model is equivalent to that of metformin in vivo.
Assuntos
Fabaceae/química , Plantas Medicinais/química , Sesquiterpenos de Eudesmano/isolamento & purificação , Fármacos Anti-HIV/química , Fármacos Anti-HIV/isolamento & purificação , Fármacos Anti-HIV/farmacologia , Cristalografia por Raios X , Glucose/metabolismo , Magnaporthe/efeitos dos fármacos , Medicina Tradicional Chinesa , Conformação Molecular , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Sesquiterpenos de Eudesmano/química , Sesquiterpenos de Eudesmano/farmacologia , EstereoisomerismoRESUMO
Two new phase I metabolites of phytoestrogen kobophenol A (1), called koboquinone A (2) and B (3), have been isolated from the feces of rats orally administered 1. Their structures were determined by spectroscopic methods. 2 also showed activity of stimulating the proliferation of cultured osteoblasts.
Assuntos
Benzoquinonas/química , Fitoestrógenos/química , Animais , Biotransformação , Proliferação de Células/efeitos dos fármacos , Espectroscopia de Ressonância Magnética , Masculino , Osteoblastos/efeitos dos fármacos , Ratos , Ratos Sprague-Dawley , Espectrometria de Massas por Ionização por Electrospray , Espectrofotometria UltravioletaRESUMO
A new resveratrol dimer, carasiphenol A (1), and a new resveratrol trimer, carasiphenol B (2), have been isolated from the aerial parts of Caragana sinica. Their structures have been elucidated from spectroscopic evidence, especially HMBC and NOE experiments. The relative configuration of the known dimer pallidol (6) was confirmed by X-ray diffraction.
Assuntos
Caragana/química , Medicamentos de Ervas Chinesas/química , Cristalografia por Raios X , Medicamentos de Ervas Chinesas/isolamento & purificação , Conformação Molecular , Estrutura Molecular , Componentes Aéreos da Planta/químicaRESUMO
AIM: To study the chemical constituents of Caragana intermedia. METHODS: The compounds were separated by chromatography methods, their structures were identified by spectral analysis. RESULTS: Ten compounds were isolated and identified as 5,7,4'-trihydroxy-3,3'-dimethoxyflavone (1), 3,5,7,8,4'-pentahydroxy-3'-methoxyflavone(2), puercetin(3), limocitrin(4), 5,7,3',4'-tetrahydroxy-3-methoxyflavone(5), 7,3',5'-trihydroxyflavanone(6), 5,7,3',4'-tetrahydroxy-3,8-dimethoxyflavone(7), butein(8), liquiritigenin(9) and 5,7,4'-trihydroxy-3,8-dimethoxyflavone(10). CONCLUSION: Compound 6 is a new compound and the others were obtained from this plant for the first time.
Assuntos
Caragana/química , Chalcona/análogos & derivados , Flavanonas/isolamento & purificação , Plantas Medicinais/química , Chalcona/química , Chalcona/isolamento & purificação , Chalconas , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/isolamento & purificação , Flavanonas/química , Flavonoides/química , Flavonoides/isolamento & purificação , Estrutura Molecular , Quercetina/química , Quercetina/isolamento & purificaçãoRESUMO
To examine the binding sites of miyabenol C (Miy C) and kobophenol A ( Kob A) with estrogen receptor (ER), computer modeling was applied to determine 3D structure of Miy C and Kob A. Molecular docking of the components to ER was carried out to find the binding sites between them. PCR mutagenesis was used to change the structure of ER cDNA. After the mutated sites were confirmed by DNA sequencing, report gene assay was used to study the effects of Miy C and Kob A on the trans-activating ability of ER. Results indicated that the effect of Miy C on the trans-activating ability of mutant 1 of ER [M1ER (ER M(517)AG(521)D)] was decreased, and Kob A had no stimulating effects on the trans-activating ability of M1ER. Miy C and Kob A had no stimulating effects on the trans-activating ability of mutant 2 of ER [M2ER (ER E(353)GR(394)G)]. Therefore, the ER sites for Miy C and Kob A may be located at Glu(353), Arg(394), Met(517) and Gly(521).
Assuntos
Receptores de Estrogênio/metabolismo , Estilbenos/metabolismo , Sequência de Bases , Sítios de Ligação/genética , Ligação Competitiva , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/metabolismo , Medicamentos de Ervas Chinesas/farmacologia , Células HeLa , Humanos , Modelos Moleculares , Dados de Sequência Molecular , Mutação , Receptores de Estrogênio/química , Receptores de Estrogênio/genética , Estilbenos/química , Estilbenos/farmacologia , Ativação Transcricional/efeitos dos fármacosRESUMO
AIM: To improve E-screen assay and make it more accurate to screen estrogenic compounds. METHODS: Estrogen receptor antisense RNA expression plasmid (pCASER) was constructed and introduced into MCF-7 with lipofectAMINE(TM), and positive clones were screened out with G418. PCR amplification was employed to identify whether estrogen receptor (ER) cDNA fragment had been inserted into MCF-7 cell genomes. Western blot was applied to detect the expression of ER. Cell growth was determined by MTT assay. RESULTS: One ER antisense clone (MTASER) had been screened out. The effects of 17beta-estradiol, genistein, droloxifen, miyabenol C, and kobophenol A on MCF-7 were stronger than those effects on MTASER. Epidermal growth factor (EGF) had equivalent stimulatory effects on the proliferation of MCF-7 and MTASER. CONCLUSION: The improved E-screen assay could screen estrogenic compounds more accurately than original E-screen assay did.