Change characteristics and influencing factors of grassland degradation in adjacent areas of the Qinghai-Tibet Plateau and suggestions for grassland restoration.

Natural grasslands are being progressively degraded around the world due to climate change and socioeconomic factors. Most of the drivers, processes, and consequences of grassland degradation are studied separately, and it is not yet clear whether the change characteristics and influence factors of adjacent areas of grassland are identical. We analyzed changes in grassland area and quality, and the influences of climate changes and socioeconomic factors from 1980-2018 in Maqu County, Xiahe County and Luqu County on the eastern Qinghai-Tibet Plateau (QTP). We found that areas with high and medium coverage grassland in Maqu County and Luqu County decreased continuously with time, while low coverage grassland areas increased in three counties. In Xiahe County, the medium coverage grassland area reduced with time (except for 2010), while the high and low coverage grassland areas increased. The actual net primary productivity of the three counties showed a downward trend. In Maqu County, the total grassland area had an extremely significant positive correlation with number of livestock going to market, commodity rate, gross domestic product (GDP), primary industry, tertiary industry, household density, and levels of junior middle school education and university education in the area. In Luqu County, the total grassland area high coverage grassland area were significantly negatively correlated with total number of livestock, secondary industry, levels of primary school education, and temperature. Ecological education was positively correlated with high coverage grassland, and negatively correlated with low coverage grassland in all three areas. The results of this study suggest that the best ways to restore the area and quality of grasslands in these areas would be to reduce the local cultivated land area and slow down the development of the primary and tertiary industries in Maqu County, and to control industry development and the total number of livestock in Luqu County. This study also suggests that improving education level and strengthening the level of ecological education are conducive to the restoration of grasslands.

In vitro characterization of glucuronidation of vanillin: identification of human UDP-glucuronosyltransferases and species differences.

Vanillin is a food flavoring agent widely utilized in foods, beverages, drugs, and perfumes and has been demonstrated to exhibit multiple pharmacological activities. Given the importance of glucuronidation in the metabolism of vanillin, the UDP-glucuronosyltransferase conjugation pathway of vanillin was investigated in this study. Vanillin glucuronide was identified by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and a hydrolysis reaction catalyzed by ß-glucuronidase. The kinetic study showed that vanillin glucuronidation by HLMs and HIMs followed Michaelis-Menten kinetics and the kinetic parameters were as follows: 134.9 ± 13.5 µM and 81.3 ± 11.3 µM for K(m) of HLMs and HIMs, 63.8 ± 2.0 nmol/min/mg pro and 13.4 ±2.0 nmol/min/mg pro for Vmax of HLMs and HIMs. All UDP-glucuronosyltransferase (UGT) isoforms except UGT1A4, 1A9, and 2B7 showed the capability to glucuronidate vanillin, and UGT1A6 exerted the higher V(max)/K(m) values than other UGT isoforms for the glucuronidation of vanillin when assuming expression of isoforms is similar in recombinant UGTs. Kinetic analysis using liver microsomes from six studied speices indicated that vanillin had highest affinity for the monkey liver microsomes enzyme (K(m) = 25.6 ± 3.2 µM) and the lowest affinity for the mice liver microsomes enzyme (K(m) = 149.1 ± 18.4 µM), and intrinsic clearance was in the following order: monkey > dog > minipig > mice > rat ~ human. These data collectively provided important information for understanding glucuronidation of vanillin.