Distinct effects of chemical- and bio- flocculants on the sludge acidogenic fermentation for volatile fatty acids production by affecting the acidogenic steps, microbial community structure and metabolic functions.

Flocculants play crucial roles in sludge treatment, while the specific impact of chemical and bio-flocculants on sludge anaerobic fermentation was unknown. This study unveiled the contrasting effects of chitosan (CTS) and poly­aluminum chloride (PAC) on volatile fatty acids (VFAs) generation during sludge fermentation. CTS supplementation resulted in 17.2 % increase in VFAs production, while PAC exposure led to 7.6 % reduction compared to the control. Further investigation revealed that CTS facilitated sludge solubilization and hydrolysis, thus providing sufficient organic substrates for VFAs generation. Additionally, environmental-friendly CTS exposure positively influenced the abundance and activity of functional anaerobes, as well as the expression of genes associated with VFAs biosynthesis. In contrast, PAC exposure resulted in the formation of larger sludge flocs, which hindered WAS solubilization and hydrolysis. Meanwhile, its potential microbial toxicity also impeded the microbial metabolic activity (i.e., genetic expressions), resulting in unsatisfactory VFAs production.

Identification of ST1 reveals a selection involving hitchhiking of seed morphology and oil content during soybean domestication.

Seed morphology and quality of cultivated soybean (Glycine max) have changed dramatically during domestication from their wild relatives, but their relationship to selection is poorly understood. Here, we describe a semi-dominant locus, ST1 (Seed Thickness 1), affecting seed thickness and encoding a UDP-D-glucuronate 4-epimerase, which catalyses UDP-galacturonic acid production and promotes pectin biosynthesis. Interestingly, this morphological change concurrently boosted seed oil content, which, along with up-regulation of glycolysis biosynthesis modulated by ST1, enabled soybean to become a staple oil crop. Strikingly, ST1 and an inversion controlling seed coat colour formed part of a single selective sweep. Structural variation analysis of the region surrounding ST1 shows that the critical mutation in ST1 existed in earlier wild relatives of soybean and the region containing ST1 subsequently underwent an inversion, which was followed by successive selection for both traits through hitchhiking during selection for seed coat colour. Together, these results provide direct evidence that simultaneously variation for seed morphology and quality occurred earlier than variation for seed coat colour during soybean domestication. The identification of ST1 thus sheds light on a crucial phase of human empirical selection in soybeans and provides evidence that our ancestors improved soybean based on taste.