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1.
Lipids ; 51(7): 807-19, 2016 07.
Artigo em Inglês | MEDLINE | ID: mdl-27270404

RESUMO

Dietary cis-9,trans-11 (c9t11) conjugated linoleic acid (CLA) fed at 0.5 % w/w was previously shown to attenuate inflammation in the murine collagen-induced (CA) arthritis model, and growing evidence implicates c9t11-CLA as a major anti-inflammatory component of dairy fat. To understand c9t11-CLA's contribution to dairy fat's anti-inflammatory action, the minimum amount of dietary c9t11-CLA needed to reduce inflammation must be determined. This study had two objectives: (1) determine the minimum dietary anti-inflammatory c9t11-CLA intake level in the CA model, and (2) compare this to anti-inflammatory effects of dairy fat (non-enriched, naturally c9t11-CLA-enriched, or c9t11-CLA-supplemented). Mice received the following dietary fat treatments (w/w) post arthritis onset: corn oil (6 % CO), 0.125, 0.25, 0.375, and 0.5 % c9t11-CLA, control butter (6 % CB), c9t11-enriched butter (6 % EB), or c9t11-CLA-supplemented butter (6 % SB, containing 0.2 % c9t11-CLA). Paw arthritic severity and pad swelling were scored and measured, respectively, over an 84-day study period. All c9t11-CLA and butter diets decreased the arthritic score (25-51 %, P < 0.01) and paw swelling (8-11 %, P < 0.01). Throughout the study, plasma tumor necrosis factor (TNFα) was elevated in CO-fed arthritic mice compared to non-arthritic (NA) mice but was reduced in 0.5 % c9t11-CLA- and EB-fed mice. Interleukin-1ß and IL-6 were increased in arthritic CO-fed mice compared to NA mice but were reduced in 0.5 % c9t11-CLA- and EB-fed mice through day 42. In conclusion, 0.125 % c9t11-CLA reduced clinical arthritis as effectively as higher doses, and decreased arthritis in CB-fed mice suggested that the minimal anti-inflammatory levels of c9t11-CLA might be below 0.125 %.


Assuntos
Anti-Inflamatórios/administração & dosagem , Artrite Experimental/dietoterapia , Gorduras na Dieta/análise , Ácidos Linoleicos Conjugados/administração & dosagem , Animais , Anti-Inflamatórios/farmacologia , Artrite Experimental/imunologia , Suplementos Nutricionais/análise , Relação Dose-Resposta a Droga , Interleucina-1beta/sangue , Interleucina-6/sangue , Ácidos Linoleicos Conjugados/farmacologia , Camundongos , Resultado do Tratamento , Fator de Necrose Tumoral alfa/sangue
2.
J Nutr ; 144(2): 177-84, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24285692

RESUMO

Dietary trans-10,cis-12 (t10c12) conjugated linoleic acid (CLA) has been shown to reduce inflammation in a murine collagen-induced arthritis (CA) model. To understand the anti-inflammatory potential of t10c12-CLA in the diet, the minimum dose of pure dietary t10c12-CLA capable of reducing CA was investigated. Because plasma inflammatory cytokines often do not reflect the progression of late-stage arthritis, inflamed tissue cytokine concentrations were also investigated in relation to increasing dietary t10c12-CLA amounts. Mice were randomly assigned to the following dietary treatments upon the establishment of arthritis: corn oil (CO) or 0.125%, 0.25%, 0.375%, or 0.5% t10c12-CLA (wt:wt) for 84 d. Sham mice (no arthritis) were fed CO and served as controls. Arthritic paw score, based on subjective assessment of arthritic severity, and paw thickness decreased linearly overall [16-65% (P < 0.001) and 0.5-12% (P < 0.001), respectively] as dietary t10c12-CLA increased (P < 0.001, R(2) < 0.81). Increasing dietary t10c12-CLA was associated with a decrease in plasma interleukin (IL)-1ß at days 21 and 42 compared with CO-fed arthritic mice, such that mice fed ≥0.25% t10c12-CLA had IL-1ß concentrations that were similar to sham mice. Plasma cytokines returned to sham mice concentrations by day 63 regardless of treatment; however, an arthritis-induced elevation in paw IL-1ß decreased linearly as dietary t10c12-CLA concentrations increased at day 84 (P = 0.007, R(2) = 0.92). Similarly, increasing dietary t10c12-CLA linearly decreased paw tumor necrosis factor (TNF)-α (P = 0.05, R(2) = 0.70). In conclusion, ≥0.125% t10c12-CLA dose-dependently reduced inflammation in a murine CA model.


Assuntos
Anti-Inflamatórios/uso terapêutico , Artrite Experimental/prevenção & controle , Dieta , Gorduras na Dieta/uso terapêutico , Interleucina-1beta/sangue , Ácidos Linoleicos Conjugados/uso terapêutico , Fator de Necrose Tumoral alfa/metabolismo , Animais , Anti-Inflamatórios/farmacologia , Artrite Experimental/sangue , Artrite Experimental/metabolismo , Colágeno , Gorduras na Dieta/farmacologia , Relação Dose-Resposta a Droga , Ácidos Linoleicos Conjugados/farmacologia , Masculino , Camundongos , Camundongos Endogâmicos DBA , Distribuição Aleatória , Índice de Gravidade de Doença
3.
Am J Physiol Regul Integr Comp Physiol ; 293(2): R669-76, 2007 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-17553852

RESUMO

A naturally occurring fatty acid, conjugated linoleic acid (CLA), reduces immune-induced TNF and inducible cyclooxygenase (COX-2) expression; key mediators of inflammation in rheumatoid arthritis (RA). On the basis of previous work, it was hypothesized that dietary CLA would act as an anti-inflammatory agent in select animal models of RA. In the collagen antibody-induced arthritis (CAIA) model, mice fed CLA (mixed isomers of c9, t11, and t10, c12-CLA) for 3 wk before anticollagen antibody injection had reduced lipopolysaccharide-induced plasma TNF levels and had arthritic scores that were 60% of mice fed corn oil (CO). In the collagen-induced arthritis (CIA) model, mice fed mixed isomers of CLA for 21 days before immunization had lower IgG(1) titers, earlier signs of joint inflammation, but similar arthritis scores compared with CO fed mice during the remaining 70-day post-injection period. Beginning on day 80 to 133, CLA-fed mice had arthritic scores 70% that of the CO-fed mice. In a second CIA experiment, CLA was fed only after the booster injection. Plasma IgG(1) levels were not reduced and arthritis onset was delayed 4 days in CLA-fed mice compared with the CO-fed mice. Peak arthritis score was similar between CLA and CO-fed mice from day 35 to 56. Because CLA reduced inflammation in the CAIA model, delayed onset of arthritis in the CIA model (CIA experiment 2) and reduced arthritis score after day 80 in the CIA model (CIA experiment 1), we concluded that dietary CLA exhibited anti-inflammatory activity that was dependent on antibody.


Assuntos
Artrite Reumatoide/dietoterapia , Artrite Reumatoide/imunologia , Ácidos Linoleicos Conjugados/imunologia , Ácidos Linoleicos Conjugados/farmacologia , Ração Animal , Animais , Artrite Experimental/dietoterapia , Artrite Experimental/imunologia , Artrite Experimental/patologia , Artrite Reumatoide/patologia , Autoanticorpos/sangue , Ciclo-Oxigenase 2/metabolismo , Gorduras na Dieta/imunologia , Gorduras na Dieta/farmacologia , Modelos Animais de Doenças , Imunoglobulina G/sangue , Lipopolissacarídeos/imunologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos DBA , Fator de Necrose Tumoral alfa/metabolismo
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