Content and gain of macro minerals in the empty body and body tissues of growing bulls.

This research aimed to generate basic data for specifying the macro mineral requirements of beef bulls. Hence, the contents of the macro minerals calcium, phosphorus, sodium, potassium, sulfur, and magnesium in the empty body and body tissue fractions of growing Fleckvieh (German Simmental) bulls with 120-780 kg live weight were determined. Results were used to calculate mineral gain rates in bulls within a wide weight range from 100 to 800 kg live weight. Calcium and phosphorus represented the largest shares in the animals' bodies. Body mineral content changed during animal growth due to progressing bone mineralization and increasing amounts of fat in all body tissues. Peak mineral gain rates were observed for calcium, phosphorus, and magnesium during the 200-400 kg live weight range. The gain rates of sodium, potassium, and sulfur declined steadily during cattle growth. The provided data allow to adjust the existing values of net mineral requirements of growing Fleckvieh bulls within the factorial requirement calculation method.

Guanidinoacetic acid as a partial replacement to arginine with or without betaine in broilers offered moderately low crude protein diets.

Guanidinoacetic acid (GAA) is the direct precursor of creatine and can spare arginine (Arg) for creatine synthesis in low crude protein (CP) broiler diets. This study aimed to determine the extent GAA could spare Arg in broilers offered low CP diets and if supplemental betaine provides additional benefits. Seven hundred twenty-day-old Ross 308 male broilers were assigned into 9 dietary treatments with 8 replicates of 10 birds each. The treatments were; normal CP diet, a low CP (-15 g/kg) diet deficient in Arg, a low CP diet sufficient in Arg, and low CP diets with GAA, where 0.1% added L-Arg was spared by GAA at 50, 100, and 150% with and without 0.1% betaine. The treatments were offered during grower (d 10-24) and finisher (d 25-42) phases. The birds offered a low CP Arg deficient diet had 7.8% lower weight gain, 10 points higher FCR, 8.5% lower breast meat yield, 27.2% lower breast meat creatine concentration and 30.4% more abdominal fat pad compared to those offered a normal CP diet. When Arg was added back to the Arg deficient diet, growth performance, breast meat yield and creatine concentration loss were restored. When GAA spared Arg at 150%, feed intake, weight gain, FCR, breast and abdominal fat yields, breast meat moisture, drip loss, and breast meat creatine concentration became comparable to Arg sufficient low CP and normal CP treatments. When GAA spared Arg at 100 and 50%, FCR was 3 and 5 points lower than the normal CP treatment. Breast meat creatine concentration was positively correlated to feed efficiency (r = 0.70, P < 0.001) and breast meat moisture (r = 0.33, P < 0.01), and negatively correlated to relative weight of abdominal fat (r = -0.37, P < 0.01) and breast meat pH (r = -0.49, P < 0.001). There were no benefits of adding betaine with GAA on the parameters measured but the results with GAA were consistent in the presence or absence of betaine.