RESUMO
We studied immunotropic properties of synthetic selenium-organic preparation 2,6-dipyridinium-9-selenabicyclo[3.3.1]nonyl dibromide (974zh). The experimental preparation reduced the cAMP/cGMP ratio, which indicated an increase in proliferative activity of cells of immunocompetent organs (thymus and spleen) in experimental animals. It was shown that 974zh intensified the immune response to Yersinia pestis EV thereby increasing the resistance to the plague agent.
Assuntos
Imunidade Inata/efeitos dos fármacos , Compostos de Selênio/farmacologia , Adjuvantes Imunológicos/química , Adjuvantes Imunológicos/farmacologia , Adjuvantes Imunológicos/uso terapêutico , Animais , Terapia Combinada , AMP Cíclico/metabolismo , GMP Cíclico/metabolismo , Feminino , Masculino , Camundongos , Compostos Orgânicos/química , Compostos Orgânicos/farmacologia , Compostos Orgânicos/uso terapêutico , Peste/tratamento farmacológico , Peste/imunologia , Peste/prevenção & controle , Vacina contra a Peste/administração & dosagem , Selênio/química , Selênio/farmacologia , Selênio/uso terapêutico , Compostos de Selênio/química , Compostos de Selênio/uso terapêutico , Baço/efeitos dos fármacos , Baço/imunologia , Baço/metabolismo , Timo/efeitos dos fármacos , Timo/imunologia , Timo/metabolismo , Potência de Vacina , Virulência/efeitos dos fármacos , Yersinia pestis/efeitos dos fármacos , Yersinia pestis/imunologia , Yersinia pestis/patogenicidadeRESUMO
The results of the study of the preparation of V. cholerae eltor membrane, obtained by the lysis and inactivation of microbial cells with urea and the subsequent differential centrifugation and nuclease treatment. As revealed in this study, the outer membrane preparation, when introduced parenterally and orally to mice, induced pronounced immunity to experimental cholera infection and the production of vibriocidal antibodies in high titers. The treatment of V. cholerae eltor membranes with trypsin led to further increase of the immunogenic potency of the preparation. The protective action of V. cholerae eltor outer membranes considerably exceeded the protective effect of currently used whole-cell eltor vaccine. This opens prospects for using the above-mentioned preparation for the improvement of chemical vaccine as a component ensuring the formation of antibacterial immunity.