RESUMO
Two aerobic, Gram-positive, rod-shaped bacterial strains, 5YN10-14(T) and GR21-5(T), were isolated from the Yongneup wetland and ginseng soil in Korea, respectively. The two strains formed ellipsoidal or oval spores positioned centrally or paracentrally in swollen sporangia. On the basis of 16S rRNA gene sequence analysis, these strains were related to members of the genus Cohnella. 16S rRNA gene sequence similarity between strains 5YN10-14(T) and GR21-5(T) was 95.9 %. Strains 5YN10-14(T) and GR21-5(T) showed, respectively, 94.3 and 95.2 % 16S rRNA gene sequence similarity to Cohnella thermotolerans CCUG 47242(T), 94.6 and 94.4 % to Cohnella hongkongensis HKU3(T), 94.7 and 94.7 % to Cohnella laeviribosi RI-39(T), and 95.4 and 94.8 % to Cohnella phaseoli GSPC1(T). The major fatty acids of strain 5YN10-14(T) were anteiso-C(15 : 0) (51.1 %), iso-C(16 : 0) (18.5 %) and C(16 : 0) (13.2 %), and the major fatty acids of strain GR21-5(T) were anteiso-C(15 : 0 ) (48.9 %), iso-C(16 : 0) (15.0 %) and iso-C(15 : 0) (12.2 %). The two strains contained menaquinone with seven isoprene units (MK-7) as the predominant quinone, and diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine as major polar lipids; however, strain 5YN10-14(T) also contained lysylphosphatidylglycerol as a major polar lipid, whereas strain GR21-5(T) had an unknown aminophospholipid as another major polar lipid. The DNA G+C contents of strains 5YN10-14(T) and GR21-5(T) were 58.8 and 61.3 mol%, respectively. Based on the results of the phylogenetic and phenotypic data presented, it was concluded that the two strains represent two novel species of the genus Cohnella , for which the names Cohnella yongneupensis sp. nov. (type strain 5YN10-14(T)=KACC 11768(T)=DSM 18998(T)) and Cohnella ginsengisoli sp. nov. (type strain GR21-5(T)=KACC 11771(T)=DSM 18997(T)) are proposed.
Assuntos
Bacillales/classificação , Bacillales/isolamento & purificação , Microbiologia do Solo , Bacillales/genética , Bacillales/metabolismo , DNA Bacteriano/genética , DNA Ribossômico/genética , Ácidos Graxos/química , Ácidos Graxos/metabolismo , Dados de Sequência Molecular , Panax/microbiologia , Filogenia , RNA Ribossômico 16S/genética , Áreas AlagadasRESUMO
Two bacterial isolates from ginseng fields in Korea, strains GR17-7(T) and GP18-1(T), were characterized using a polyphasic approach. Phylogenetic analysis of their 16S rRNA gene sequences revealed a clear affiliation with the Gammaproteobacteria, and showed that the closest phylogenetic relationships were with members of the genus Rhodanobacter. The 16S rRNA gene sequence similarity between strains GR17-7(T) and GP18-1(T) was 97.2 %. Both strains showed 16S rRNA gene sequence similarities of 95.2-96.9 % to type strains of recognized Rhodanobacter species. The G+C contents of the DNA of strains GR17-7(T) and GP18-1(T) were 61.0 and 62.5 mol%, respectively. According to the DNA-DNA hydridization tests, the hybridization value between strains GR17-7(T) and GP18-1(T) was 34 %. Strains GR17-7(T) and GP18-1(T) showed less than 32 % DNA-DNA relatedness with Rhodanobacter fulvus KCTC 12098(T) and Rhodanobacter spathiphylli LMG 23181(T). Strains GR17-7(T) and GP18-1(T) were aerobic, Gram-negative, rod-shaped, and catalase- and oxidase-positive. Major fatty acids of both strains were iso-C(17 : 1)omega9c and iso-C(16 : 0). Based on the data presented, two novel Rhodanobacter species are proposed, with the names Rhodanobacter ginsengisoli sp. nov. (type strain GR17-7(T)=KACC 11762(T)=DSM 18993(T)) and Rhodanobacter terrae sp. nov. (type strain GP18-1(T)=KACC 11761(T)=DSM 19241(T)).