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OBJECTIVE@#To compare the clinical efficacy and safety between syndrome-differentiation acupuncture combined with rehabilitation training and simple rehabilitation training for children with autism spectrum disorder (ASD).@*METHODS@#A total of 60 children with ASD were randomly divided into an observation group and a control group, 30 cases in each group. In the control group, routine rehabilitation training was applied; in the observation group, syndrome-differentiation acupuncture (the main points were Baihui [GV 20], Dingshenzhen, Niesanzhen, etc., the supplementary acupoints were selected according to syndrome-differentiation) combined with rehabilitation training were applied, all the treatments were given once a day, 5-day continuous treatment with 2-day interval, 12 weeks were required. Before treatment and after 6, 12 weeks of treatment, the autism treatment evaluation checklist (ATEC), childhood autism rating scale (CARS) and autism behavior checklist (ABC) scores were observed, the therapeutic effect and safety were evaluated in the two groups.@*RESULTS@#After 6 and 12 weeks of treatment, except for the sensory perception score after 6 weeks of treatment in the control group, the item scores and total scores of ATEC, CARS scores and ABC scores were decreased compared with those before treatment in the two groups (P<0.05). After 6 weeks of treatment, the social score and total score of ATEC, CARS score in the observation group were lower than those in the control group (P<0.05); after 12 weeks of treatment, the item scores and total score of ATEC, CARS score and ABC score in the observation group were lower than those in the control group (P<0.05). The total effective rate in the observation group was 80.0% (24/30), which was higher than 56.7% (17/30) in the control group (P<0.05). There was no serious adverse reactions in the two groups, and there was no significant difference in the incidence rate of adverse reactions between the two groups (P>0.05).@*CONCLUSION@#Syndrome-differentiation acupuncture combined with rehabilitation training could improve the core symptoms in children with ASD, especially sensory perception and social ability, and with good safety, which is superior to simple rehabilitation training.
Assuntos
Criança , Humanos , Transtorno do Espectro Autista/terapia , Terapia por Acupuntura , Resultado do Tratamento , Pontos de Acupuntura , MedicinaRESUMO
OBJECTIVE@#To investigate the clinical efficacy and safety of percutaneous foraminal endoscopy in the treatment of lumbar lateral recess stenosis in elderly.@*METHODS@#The clinical data of 31 elderly patients with lumbar lateral recess stenosis treated by percutaneous foraminal endoscopic decompression from March 2018 to August 2019 were retrospectively analyzed. Including 16 males and 15 females, aged from 65 to 81 years with an average of (71.13±5.20) years, the course of disease ranged from 3 months to 7 years with an average of (14.36±6.52) months. Visual analogue scale (VAS) and Oswestry disability index (ODI) were used to assess clinical symptom and functional status before operation and 1, 6, 12 months after operation. At the final follow-up, the modified Macnab standard was used to evaluate clinical efficacy.@*RESULTS@#All patients were completed the operation successfully. The operation time was from 75 to 120 min with an average of (97.84±11.22 ) min. All 31 patients were followed up from 12 to 28 months with an average of (17.29±5.56) months. Postoperative lumbago-leg pain VAS and ODI were significantly improved at 1, 6, and 12 months(P<0.01). At the final follow-up, according to the modified Macnab standard to evaluate the effect, 23 got excellent results, 5 good, 3 fair. One patient had severe adhesions between peripheral tissues and nerve root, and postoperative sensory abnormalities in the lower extremities were treated conservatively with traditional Chinese medicine and neurotrophic drugs, which recovered at 2 weeks after surgery. No complications such as nerve root injury and infection occurred.@*CONCLUSION@#The intervertebral foraminal endoscopy technique, which is performed under local anesthesia for a short period of operation, ensures adequate decompression while minimizing complications, and is a safe and effective surgical procedure for elderly patients with lumbar lateral recess stenosis.
Assuntos
Masculino , Feminino , Humanos , Idoso , Lactente , Constrição Patológica/cirurgia , Estenose Espinal/cirurgia , Descompressão Cirúrgica/métodos , Estudos Retrospectivos , Vértebras Lombares/cirurgia , Endoscopia/métodos , Resultado do TratamentoRESUMO
Yin-deficiency-heat (YDH) syndrome is a very common subhealth status in Traditional Chinese Medicine. However, currently, there is no unified standard for diagnosing YDH syndrome. We applied the iTRAQ-2D LC-MS/MS method to explore the potential of serum protein profiles as biomarker for YDH syndrome. A total of 120 differentially expressed proteins (79 downregulated and 41 upregulated) were identified by the proteomic profiling. The results of KEGG pathway analysis showed that the functions of the differentially expressed proteins were mainly involved in complement and coagulation cascades. The clinical data showed that YDH syndrome was closely related to inflammation and coagulation, compared with the healthy controls. The ELISA validation results indicated that the expression levels of ALB, CFI, and KLKB1 were downregulated in the YDH syndrome group (p < .05). Moreover, we established a decision tree model based on the combination of these three proteins and achieved a sensitivity of 87.5%, a specificity of 84.4%, and AUC of 0.891. The results indicated that the combination of ALB, CFI, and KLKB1 may serve as potential biomarkers for diagnosing YDH syndrome. Our study can provide a new method for YDH syndrome diagnosis, and may also provide an experimental basis to understand the molecular mechanism of YDH syndrome.
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Proteínas Sanguíneas/metabolismo , Medicina Tradicional Chinesa , Úlceras Orais/diagnóstico , Deficiência da Energia Yin/diagnóstico , Adulto , Biomarcadores/sangue , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Úlceras Orais/sangue , Proteômica , Espectrometria de Massas em Tandem , Deficiência da Energia Yin/sangueRESUMO
For decades, intravenous immunoglobulin (IVIg) has provided safe and effective therapy for immunodeficient patients. This proof-of-principle study describes a novel approach to generate personalized IVIg for chronic, antibiotic-resistant infection in real time.
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Antibacterianos/uso terapêutico , Terapia Biológica , Imunoglobulinas Intravenosas/uso terapêutico , Infecções por Mycoplasma/terapia , Medicina de Precisão , Infecção da Ferida Cirúrgica/terapia , Idoso , Animais , Bovinos , Humanos , Imunoglobulinas Intravenosas/efeitos adversos , Síndromes de Imunodeficiência/terapia , Masculino , Infecções por Mycoplasma/diagnóstico , Mycoplasma hominis/efeitos dos fármacos , Estudo de Prova de Conceito , Infecção da Ferida Cirúrgica/microbiologiaRESUMO
OBJECTIVE: To observe the effect of electroacupuncture (EA) and manual acupuncture interventions on colonic inflammatory injury, cytokine level and cell apoptosis in ulcerative colitis (UC)rats, so as to reveal its mechanisms underlying improvement of UC. METHODS: A total of 32 SD rats were randomized into control, model, EA and manual acupuncture groups (8 rats/group). The UC model was established by intrarectally administration of 5% trinitro-benzene-sulfonic acid (TNBS)+ dehydrated alcohol. Both "Quchi" (LI 11) and "Zusanli" (ST 36) were punctured with filiform needles for 20 min in the manual acupuncture group or stimulated with EA (8 Hz/25 Hz, 2-4 mA, and duration of 20 min) in the EA group. The treatment was conducted once daily for consecutive 6 days. Changes of body weight and pathological state of colitis were observed. The contents of pro-inflammatory cytokines (TNF-α, IL-1 ß, IL-6), anti-inflammatory cytokine (IL-10), homocysteine (Hcy) and myeloperoxidase (MPO, two oxygen free radicals associated substances) in the colon tissues were detected by ELISA, and the protein expression levels of Bcl-2,Bax,phosphorylated (p)-inhibitor of nuclear factor kappaB kinase α(IκBα) and p-p 65 (a subunit of nuclear factor) of colonic tissues were detected by Western blot, separately. RESULTS: Compared with the control group, the body weight was decreased and the state of the swelling and hemorrhage of the colon got worsened in the model group, while the state of the swelling and hemorrhage of the colon was better in both EA and manual acupuncture groups, and the body weight was clearly increased from day 4 on in both treatment groups. The concentrations of colonic TNF-α, IL-1 ß, IL-6, IL-10, MPO and Hcy were all significantly higher in the model group than in the control group (P<0.01), but those of colonic TNF-α, IL-1 ß, IL-6 in both EA and manual acupuncture groups, those of MPO and Hcy in the EA group were significantly down-regulated following the intervention (P<0.05, P<0.01), and that of IL-10 was notably further increased in the manual acupuncture group (P<0.05). In addition, modeling-induced remarkable down-regulation of colonic Bcl-2/Bax, and marked up-regulation of expression of IκBα and p-p 65 proteins were significantly suppressed in both EA and manual acupuncture groups (P<0.01). CONCLUSIONS: Both EA and manual acupuncture interventions Feb alleviate the colonic lesions in UC rats, which Feb be related to their functions in regulating levels of pro-inflammatory and anti-inflammatory cytokines, in balancing the expression of apoptosis-related protein and anti-apoptosis-related protein and in down-regulating the expression of the key nuclear transcription factors in the colonic tissue.
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Colite Ulcerativa/fisiopatologia , Colite Ulcerativa/terapia , Citocinas/imunologia , Eletroacupuntura , Animais , Apoptose , Colite Ulcerativa/genética , Colite Ulcerativa/imunologia , Colo/imunologia , Citocinas/genética , Humanos , Interleucina-10/genética , Interleucina-10/imunologia , Interleucina-1beta/genética , Interleucina-1beta/imunologia , Interleucina-6/genética , Interleucina-6/imunologia , Masculino , Ratos , Ratos Sprague-Dawley , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/imunologiaRESUMO
Thalassemia is inherited hemolytic anemia caused by globin production disorder,clinically known as beta thalassemia and alpha thalassemia resulted from beta and alpha chain synthesis disorder.Significant anemic clinical manifestations are observed in intermediate -level and severe -level patients with the 2 types of anemia, affecting greatly the growth and life quality of children patients who need blood transfusion to stay alive.The incidence rate of thalassemia is as high as 7% -20% in South China,so the prevention and standaridized treatment of thalasse-mia and the management over target population draw more attention.This paper is a review on the latest development of blood transfusion,chelation therapy,health education,birth intervention for thalassemia.
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Triptolide is used in traditional Chinese medicine. It has the advantages of a unique mechanism of action, a wide antitumor spectrum, multiple targets, multi-channel effects and low toxicity. The current study was conducted to evaluate whether the potential anticancer effects of triptolide reduces proliferation and enhances apoptosis of human nonsmall cell lung cancer (NSCLC) cells, and to assess the underlying anticancer mechanisms. In PC9 cells, treatment with triptolide reduced cell proliferation and increased cell apoptosis and caspase3 and 9 activity. Triptolide treatment reduced miR21 expression and enhanced phosphatase and tensin homolog (PTEN) protein expression levels in the PC9 cells. Furthermore, the upregulation of miR21 expression levels suppressed the effect of triptolide on cell viability and PTEN protein expression levels in PC9 cells. To the best of our knowledge, the present study is the first to demonstrate that triptolide reduced the proliferation and enhanced the apoptosis of human NSCLC cells through PTEN by targeting miR-21.
Assuntos
Antineoplásicos/farmacologia , Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Diterpenos/farmacologia , MicroRNAs/fisiologia , PTEN Fosfo-Hidrolase/genética , Fenantrenos/farmacologia , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Ensaios de Seleção de Medicamentos Antitumorais , Compostos de Epóxi/farmacologia , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/metabolismo , PTEN Fosfo-Hidrolase/metabolismo , Interferência de RNARESUMO
Tanshinone IIA is known to induce apoptosis in several types of cancer cells. However, little is known about its activity in chemoresistant cells. The aim of this study was to investigate the anticancer properties of tanshinone IIA in cisplatin-resistant human ovarian cancer COC1/DDP cells in vitro. We used a variety of methods to measure cell viability, the resistance index (RI) of cisplatin, cellular apoptosis, p38 mitogen-activated protein kinase (MAPK) expression and phosphorylation, and the mRNA expression of several genes implicated in drug resistance including survivin, Caspase-3, excision repair cross-complementing gene 1 (ERCC1), multidrug resistance (MDR), lung resistance protein (LRP) and glutathione-S-transferase-π (GST-π). We found that tanshinone IIA time- and dose-dependently inhibited the proliferation of COC1/DDP cells and caused significant apoptosis. Western blotting revealed that tanshinone IIA also increased phospho-p38 MAPK in a time- and dose-dependent manner. After treatment by tanshinone IIA for 48 h, the RI of cisplatin and the mRNA expression of survivin, ERCC1 and LRP were all significantly decreased. Furthermore, blockade of p38 signal transduction decreased apoptotic cell rates and dramatically elevated the mRNA expression of the survivin, ERCC1 and LRP genes. We therefore conclude that tanshinone IIA induces apoptosis and reduces cisplatin resistance in COC1/DDP cells and thus causes significant growth inhibitory effects. This mechanism appears to involve p38-mediated downregulation of survivin, ERCC1 and LRP mRNA expression.