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1.
Food Chem ; 416: 135870, 2023 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-36898342

RESUMO

Tannins are involved in the taste of foods and multi bioactivity of traditional herbal medicines. The characteristics of tannins are believed to derive from their connectivity with proteins. However, the mode of interaction between proteins and tannins is not yet understood because of the complexity of the tannin structure. Then this study aimed to elucidate the detail binding mode of tannin and protein by the 1H-15N HSQC NMR method using the 15N-labeled MMP-1that have not been used so far. The HSQC results suggested cross-link sites between MMP-1s, which cause protein aggregation and inhibit MMP-1 activity. This study presents the first 3D protein aggregation model of condensed tannins, which is important for understanding the bioactivity of polyphenols. Furthermore, it can broaden the understanding of the range of interactions between other proteins and polyphenols.


Assuntos
Plantas Medicinais , Proantocianidinas , Agregados Proteicos , Taninos/química , Polifenóis/farmacologia , Plantas Medicinais/metabolismo
2.
Biosci Biotechnol Biochem ; 76(8): 1571-2, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22878196

RESUMO

A new type of flavonoid was isolated from an adzuki bean-water extract by various chromatographic techniques. The chemical structure was determined by ultraviolet-visible (UV-Vis) spectroscopy, mass spectrometry (MS), and various nuclear magnetic resonance (NMR) experiments, and it was found to be unique in that the xanthylium skeleton was present in this molecule.


Assuntos
Produtos Biológicos/isolamento & purificação , Fabaceae/química , Flavonoides/isolamento & purificação , Compostos Heterocíclicos com 3 Anéis/isolamento & purificação , Extratos Vegetais/química , Produtos Biológicos/química , Flavonoides/química , Compostos Heterocíclicos com 3 Anéis/química , Extração Líquido-Líquido , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Espectrofotometria
3.
Antimicrob Agents Chemother ; 53(2): 765-71, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19015328

RESUMO

Transmissible spongiform encephalopathies are associated with the conformational conversion of the prion protein from the cellular form (PrP(C)) to the scrapie form. This process could be disrupted by stabilizing the PrP(C) conformation, using a specific ligand identified as a chemical chaperone. To discover such compounds, we employed an in silico screen that was based on the nuclear magnetic resonance structure of PrP(C). In combination, we performed ex vivo screening using the Fukuoka-1 strain-infected neuronal mouse cell line at a compound concentration of 10 microM and surface plasmon resonance. Initially, we selected 590 compounds according to the calculated docked energy and finally discovered 24 efficient antiprion compounds, whose chemical structures are quite diverse. Surface plasmon resonance studies showed that the binding affinities of compounds for PrP(C) roughly correlated with the compounds' antiprion activities, indicating that the identification of chemical chaperones that bind to the PrP(C) structure and stabilize it is one efficient strategy for antiprion drug discovery. However, some compounds possessed antiprion activities with low affinities for PrP(C), indicating a mechanism involving additional modulation factors. We classified the compounds roughly into five categories: (i) binding and effective, (ii) low binding and effective, (iii) binding and not effective, (iv) low binding and not effective, and (v) acceleration. In conclusion, we found a spectrum of compounds, many of which are able to modulate the pathogenic conversion reaction. The appropriate categorization of these diverse compounds would facilitate antiprion drug discovery and help to elucidate the pathogenic conversion mechanism.


Assuntos
Doenças Priônicas/tratamento farmacológico , Príons/antagonistas & inibidores , Príons/química , Animais , Linhagem Celular , Simulação por Computador , Bases de Dados Genéticas , Avaliação Pré-Clínica de Medicamentos , Humanos , Ligantes , Camundongos , Proteínas Recombinantes/antagonistas & inibidores , Proteínas Recombinantes/química , Ressonância de Plasmônio de Superfície
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