RESUMO
OBJECTIVE: To investigate the hemostatic effect of modified Sijunzi Granules (MSG) in primary immune thrombocytopenia (ITP) zebrafish model and explore the potential mechanism. METHODS: AB strain wild type zebrafish were treated with simvastatin (6 µmol/L) for 24 h to establish the hemorrhage model (model control group). The zebrafish were treated with MSG at different doses (55.6, 167, and 500 µg/mL), respectively. The hemostatic effect was assessed by examining the intestinal bleeding and hemostatic rate. 5-Hydroxytryptamine (5-HT) content was determined using enzyme-linked immunosorbent assay (ELISA) assay. The expressions of 5-HT2aR, 5-HT2bR, and SERT genes were detected by quantitative real-time polymerase chain reaction(PCR). The protein expressions of protein kinase B (Akt), p-Akt, extracellular regulated protein kinases (Erk), and p-Erk were examined using Western blot analysis. RESULTS: The intestinal bleeding rate was 37%, 40%, and 80% in the 55.6, 167, and 500 µg/mL dose of MSG, respectively, in which 55.6 and 167 µg/mL MSG dose groups were associated with significantly decreased intestinal bleeding rate when compared with the model control group (70%, P<0.05). Significantly higher hemostatic rates were also observed in the 55.6 (54%) and 167 (52%) µg/mL MSG dose groups (P<0.05). MSG increased the 5-HT content and mRNA expression levels of 5-HT2aR, 5-HT2bR, and SERT (P<0.05). In addition, caspase3/7 activity was inhibited (P<0.05). Significant increase in p-Akt and p-Erk was also detected after treatment with MSG (P<0.05). CONCLUSIONS: MSG could reduce the incidence and severity of intestinal bleeding in zebrafish by activating MAPK/Erk and PI3K/Akt signal pathways through regulating the levels of 5-HT and its receptors, which may provide evidence for the treatment of ITP.
RESUMO
Background: There are many kinds of medicinal animal resources, which are an important part of traditional Chinese medicine resources (TCM). However, the use of medicinal animals in TCM, especially wild animals, has become a sensitive problem at home and abroad. Systematic analysis on the research status and direction of medical animals in the last 10 years which for promoting the sustainable development of Chinese medicine. Methods: PubMed, Web of Science, Embase, CINAHL, CNKI, VIP database and WanFang Database were selected, and SPSS 25.0 software was used to analyze annual publications, journals, global distribution, authors, coauthors and co-authors rate, author institutions and high-frequency keywords. Results: Chinese Journal of modern Chinese medicine occupies the majority articles with a high co-authorship rate, but low impact factors. The development of medical animals around the world is not balanced. The top three countries are China, United Kingdom and United States. However, these countries have less exchanges and cooperation with each other. The Institute of TCM of Chinese Academy owns the most research achievements. At present, the hot spots involve the identification and quality of medical animals, applied basic research. Conclusion: The identification, quality and applied basic research of medical animals are still worthy of increasing research investment. In addition, it is necessary to strengthen exchanges and international cooperation among different countries in TCM, and promote the high-quality development in medical animals.