Gastric cancer patient-derived organoids model for the therapeutic drug screening.

BACKGROUND: Gastric cancer (GC) is a highly heterogeneous disease featuring many various histological and molecular subtypes. Therefore, it is imperative to have well-characterized in vitro models for personalized treatment development. Gastric cancer patient-derived organoids (PDOs), re-capitulating in vivo conditions, exhibit high clinical efficacy in predicting drug sensitivity to facilitate the development of cancer precision medicine. METHODS: PDOs were established from surgically resected GC tumor tissues. Histological and molecular characterization of PDOs and primary tissues were performed via IHC and sequencing analysis. We also conducted drug sensitivity tests using PDO cultures with five chemotherapeutic drugs and twenty-two targeted drugs. RESULTS: We have successfully constructed a PDOs biobank that included EBV+, intestinal/CIN, diffuse/GS, mixed and Her2+ GC subtypes, and these PDOs captured the pathological and genetic characteristics of corresponding tumors and exhibited different sensitivities to the tested agents. In a clinical case study, we performed an additional drug sensitivity test for a patient who reached an advanced progressive stage after surgery. We discovered that the combination of napabucasin and COTI-2 exhibited a stronger synergistic effect than either drug alone. CONCLUSION: PDOs maintained the histological and genetic characteristics of original cancer tissues. PDOs biobank opens up new perspectives for studying cancer cell biology and personalized medicine as a preclinical study platform.

Estimating the catechin concentrations of new shoots in green tea fields using ground-based hyperspectral imagery.

Hyperspectral imagery was applied to estimating non-galloyl (EC, EGC) and galloyl (ECG, EGCG) types of catechins in new shoots of green tea. Partial least squares regression models were developed to consider the effects of commercial fertilizer (CF) and organic fertilizer (OF). The models could explain each type of catechin with a precision of more than 0.79, with a few exceptions. When the CF model was applied to the OF hyperspectral reflectance and the OF model was applied to the CF hyperspectral reflectance for mutual prediction, the prediction accuracy was better with the OF models than CF models. The prediction models using both CF and OF data (hyperspectral reflectances, and concentrations of catechins) had a precision of more than 0.76 except for the non-galloyl-type catechins as a group and EGC alone. These results provide useful data for maintaining and improving the quality of green tea.

Clinical observation on moxibustion at Baihui (GV20) plus Tuina for children with postnasal drip syndrome / 针灸推拿医学（英文版）

Objective: To observe the clinical efficacy of moxibustion at Baihui (GV20) plus Tuina (Chinese therapeutic massage) for children with postnasal drip syndrome (PNDS).Methods: A total of 60 cases were divided into an observation group and a control group according to the random number table method, with 30 cases in each group. Cases in the control group received mometasone furoate nasal spray and nasal irrigation with 0.9％ normal saline. Cases in the observation group received additional moxibustion at Baihui (GV20) plus Tuina at the head and face. All the cases were treated for four consecutive weeks. The clinical efficacy and traditional Chinese medicine (TCM) symptoms score were compared between the two groups. Results: The total effective rate of the observation group was 93.3％ while that of the control group was 70.0％. The difference between the two groups was statistically significant (P<0.05). After treatment, the TCM symptom scores in both groups declined significantly, showing a statistical difference from that before treatment (P<0.05), and the score of the observation group was notably lower than that of the control group (P<0.05). Conclusion: The clinical efficacy of moxibustion at Baihui (GV20) plus Tuina at the head and face on the basis of nasal spray with Western medicine is more significant than that of Western medicine alone for PNDS in children.

Exploring the preconception of the first year of medical students on medicine before entering medical school.

PURPOSE: First-year students can form a preconception based on life experiences before entering college and identifying learners' existing characteristics can be useful foundation data for curriculum development. This study examines what preconceptions freshman students had about medicine before entering medical school. METHODS: A total of 110 first-year students were asked about what preconceptions they had about "medicine". A total of 1,124 data were used in the content analysis method. RESULTS: The results were extracted into 5, and 12 twelve categories. On the theme of "scientific discipline", the knowledge students had about general health was based on scant expertise and little evidence. Students perceived medicine as Western and scientific, considering Korean traditional medicine as unscientific. Students believed that "medical practice" should be a "disease treatment" and "patient-centered" approach rather than a "social responsibility". In "the role of the doctor", students were concerned about the doctor's being financially stable on the positive side, and about the high-intensity workload on the negative side. In "medical education", students believed that studying medicine would be "hard and difficult" because of the "importance of memorizing" and "extensive study load". In "specialty stereotype", students had biases that were mostly concentrated on "psychiatry" and "surgery". CONCLUSION: Perception of "medicine" has been revealed to a varied range of themes, but some have been inaccurate or unrealistic. These prejudices and groundless beliefs have a gap with the learning outcomes that students should achieve in the curriculum, and these preconceptions seem to have been influenced by South Korea's unique cultural context.

Observation on clinical effects of tuina plus montelukast sodium tablets for children with cough variant asthma / 针灸推拿医学（英文版）

Objective:To observe the clinical effects of tuina plus oral administration of montelukast sodium tablets for children with cough variant asthma (CVA). Methods:A hundred and six children with CVA were randomly divided into a treatment group and a control group, 53 cases in each group. The treatment group was treated with Tuina therapy plus oral administration of montelukast sodium tablets, and the control group was treated only by oral administration of montelukast sodium tablets. Tuina treatment was given three times per week. 5 mg of montelukast sodium tablets was given orally before bedtime every night. The therapeutic effects were assessed after the treatment of four weeks in both groups. Results:The total effective rates were 92.5% in the treatment group and 77.4% in the control group. The difference in the total effective rate between the two groups was statistically significant (P<0.05). Conclusion:The therapeutic effect of tuina plus montelukast sodium tablets for children with CVA is better than that of oral administration of montelukast sodium tablets alone.

Observation on clinical effects of tuina plus montelukast sodium tablets for children with cough variant asthma / 针灸推拿医学（英文版）

Objective:To observe the clinical effects of tuina plus oral administration of montelukast sodium tablets for children with cough variant asthma (CVA). Methods:A hundred and six children with CVA were randomly divided into a treatment group and a control group, 53 cases in each group. The treatment group was treated with Tuina therapy plus oral administration of montelukast sodium tablets, and the control group was treated only by oral administration of montelukast sodium tablets. Tuina treatment was given three times per week. 5 mg of montelukast sodium tablets was given orally before bedtime every night. The therapeutic effects were assessed after the treatment of four weeks in both groups. Results:The total effective rates were 92.5% in the treatment group and 77.4% in the control group. The difference in the total effective rate between the two groups was statistically significant (P<0.05). Conclusion:The therapeutic effect of tuina plus montelukast sodium tablets for children with CVA is better than that of oral administration of montelukast sodium tablets alone.

[Clinical observation on treatment of gastrointestinal dysfunction by fu'an liquid for retention enema in children with critical illness].

OBJECTIVE: To observe the therapeutic effect of Fu'an Liquid (FAL) for retention enema in treating gastrointestinal (GI) dysfunction of children with critical illness. METHODS: Eighty-nine patients were randomly divided into two groups, 52 in the treated group and 37 in the control group. Conventional therapy of western medicine was given to both groups and to the treated group FAL was given additionally. Plasma endothelin (ET) level was measured during admission, GI dysfunction occurrence and after treatment, and the therapeutic effect as well as the recovery of GI condition were observed. RESULTS: The total effective rate of FAL in treating GI dysfunction was 84.62%, which was significantly higher than that in the control group (62.17%) (P < 0.05). In the treated group, 34 cases were treated successfully, 16 died and the other 2 abandoned, the mortality rate being 30.77%, while in the control group, the corresponding numbers were 16, 18, 3 and 48.65%. The mortality rate in the treated group was lower than that in the control group (chi 2 = 4.64, P < 0.05). Level of ET in both groups was higher than normal range during admission (P < 0.01), it further increased when GI dysfunction occurred (P < 0.01), and decreased when successfully treated, the decrease was quicker in the treated group than that in the control group (P < 0.05). CONCLUSION: In children with critical illness, ET level would increase when the patient was complicated with GI dysfunction. FAL for retention enema could reduce the ET level effectively, promote the recovery of patients from GI dysfunction, so as to play a definite role in enhancing the successful rate of rescue.

Clinical observation on treatment of gastrointestinal dysfunction by fu'an liquid for retention enema in children with critical illness / 中国中西医结合杂志

<p><b>OBJECTIVE</b>To observe the therapeutic effect of Fu'an Liquid (FAL) for retention enema in treating gastrointestinal (GI) dysfunction of children with critical illness.</p><p><b>METHODS</b>Eighty-nine patients were randomly divided into two groups, 52 in the treated group and 37 in the control group. Conventional therapy of western medicine was given to both groups and to the treated group FAL was given additionally. Plasma endothelin (ET) level was measured during admission, GI dysfunction occurrence and after treatment, and the therapeutic effect as well as the recovery of GI condition were observed.</p><p><b>RESULTS</b>The total effective rate of FAL in treating GI dysfunction was 84.62%, which was significantly higher than that in the control group (62.17%) (P < 0.05). In the treated group, 34 cases were treated successfully, 16 died and the other 2 abandoned, the mortality rate being 30.77%, while in the control group, the corresponding numbers were 16, 18, 3 and 48.65%. The mortality rate in the treated group was lower than that in the control group (chi 2 = 4.64, P < 0.05). Level of ET in both groups was higher than normal range during admission (P < 0.01), it further increased when GI dysfunction occurred (P < 0.01), and decreased when successfully treated, the decrease was quicker in the treated group than that in the control group (P < 0.05).</p><p><b>CONCLUSION</b>In children with critical illness, ET level would increase when the patient was complicated with GI dysfunction. FAL for retention enema could reduce the ET level effectively, promote the recovery of patients from GI dysfunction, so as to play a definite role in enhancing the successful rate of rescue.</p>