Effect of different nitrogen forms and ratio on growth and active ingredient content of Platycodon grandiflorum / 中国中药杂志

To providing evidence about nitrogen adequate application of Platycodon grandiflorum, the pot culture experiment was conducted to study the effect of nitrogen on the growth, physiological metabolism and the quality of P. grandiflorum. The activity of NR, GS and SOD, POD and CAT were determined. And the nitrate and ammonium nitrogen content, photosynthetic characteristics, active components of P. grandiflorum were determined. The results showed that the nitrate nitrogen content and P. biomass reached its maximum value, when NH4(+)-N/NO3(-) -N was 0: 100, the activity of NR. The activity of GS was the highest at the NH4(+) -N/NO3(-) -N ratio of 25:75 and ammonium nitrogen content was the highest at 75:25. The activity of SOD decreased and then increased with the increasing of NO3(-) -N. At the NH4(+) -N/NO3(-) -N ratio of 25: 75, the activity of CAT had its maximum value and the content of MDA had the minimum value. At the same time, the content of platycodon D was the highest at this treatment. The studies had shown that different nitrogen forms and ratio had a significant effect on the characteristics of photosynthetic physiology, nitrogen metabolism and resistance adjustment, growth and the quality of P. grandiflorum. The NH4(+) -N/NO3(-) -N ratio of 25: 75 was a suitable ratio of nitrogen forms for the growth of P. Grandiflorum and accumulating the content of platycodon D.

Karyotype and pollen morphology of main Fritillaria thunbergii cultivars / 中国中药杂志

<p><b>OBJECTIVE</b>To study the differences in pollen morphology and karyotype among main Fritillari thunbergii cultivars.</p><p><b>METHOD</b>Pollen morphologies of three F. thunbergii cultivars were examined by scanning electron microscopy (SEM), and the chromosome numbers and karyotypes were studied by applying traditional squash technique.</p><p><b>RESULT</b>The pollen shape of F. thunbergii (Xiaye) was ovoid, while those of the other F. thunbergii (Kuanye, Duozi) were oblong. There were significant differences in mesh ridge width, mesh size among three F. thunbergii cultivars. The karyotype formula ofthree cultivars were as follows: F. thunbergii (Xiaye) was 2n =2x =3m +1sm + 8st(2SAT) + 12t(4SAT), F. thunbergii (Kuanye) was 2n = 2x =2m + 2sm + 10st(2SAT) + 10t (2SAT), F. thunbergii (Duozi) was 2n =2x = 24 =2m +2sm +5st(2SAT) +15t(4SAT). The three cultivars of karyotype belonged to 3B; There were the heterozygosity of homologous chromosome in both F. thunbergii (Xiaye) and F. thunbergii (Duozi).</p><p><b>CONCLUSION</b>The genetic diversity of F. thunbergii is very rich, which could enhance the adaptability, and lay the foundations for new variety selection of F. thunbergii.</p>

Effect of different nitrogen forms and concentrations on biomass and alkaloids of isatidis folium / 中国中药杂志

<p><b>OBJECTIVE</b>In order to provide theoretical basis of improving nitrogen utilization efficiency in Isatis indigotica, the biomass and active components in Isatidis Folium under different nitrogen forms and concentrations were analyzed.</p><p><b>METHOD</b>I. indigotica was cultivated by sand culture in greenhouse, and the experiment was designed with orthogonal test L25 (5(6)). As an index to the biomass and indigo, indirubin, the effects on the I. indigotica by three factors [NO3(-) -N, NH4(+) -N, CO(NH2)2] at five different levels were studied.</p><p><b>RESULT</b>There were significant difference of the biomass and active components by different nitrogen forms and concentrations. The effect of amide nitrogen [CO(NH2)2] on biomass of Isatidis Folium was the most apparent, and the effect of ammonium nitrogen on indirubin was more obvious. Considering the biomass and active ingredient, one combination was optimized, which was (NH4)2SO(4)-7.5 mmol x L(-1), KNO(3)-2.5 mmol x L(-1), CO(NH2)(2)- 5 mmol x L(-1).</p><p><b>CONCLUSION</b>It is important to promote the growth in pre-stage of I. indigotica, and cost-effective combination of balanced nitrogen fertilizer could reasonably promote the growth, and improve the contents of active components and individual biomass.</p>

Mechanism of copper and zinc on tanshinones of cultivated Salvia miltiorrhiza / 中国中药杂志

<p><b>OBJECTIVE</b>To study the mechanism of microelement including Cu and Zn on the accumulation of three danshinones in Salviae miltiorrhizae root and build a theory basis for its good quality and high yield.</p><p><b>METHOD</b>Sand culture experiments were conducted to study the effect of Cu and Zn on the accumulation of three danshinones and oxidase including peroxidase and polyphenol oxidase activity in the plant root. The correlation between available Cu and Zn contents in matrix and oxidase activity in the plant root and, the correlation between available Cu and Zn contents in matrix and contents of tanshinones in the root were discussed.</p><p><b>RESULT</b>Contents of danshinones in the root increased with the increasing of Cu and Zn concentration. Dynamic monitoring on contents of dan-shinones of the plant roots growing in the pots with different Cu and Zn concentration in the whole growing season showed that the contents of danshinones for 60 days were the lowest, for 120 days the highest and then dropped for 150 days. In addition, among available Cu and Zn contents of matrix, oxidase including peroxidase and polyphenol oxidase activity and contents of tanshinones in the root,the correlation between two factors were significant difference (P < 0.05 or P < 0.01).</p><p><b>CONCLUSION</b>The mechanism of Cu and Zn on the accumulation of danshinones may be that Cu and Zn improve the activity of peroxidase and polyphenol oxidase, which promote transformation of phenolic compounds to terpenes and therefore to increase contents of danshinones.</p>

Effects of formula fertilization to yield and content on polysaccharide of Isatis indigotica / 中国中药杂志

<p><b>OBJECTIVE</b>To select the optimum formula fertilization of Isatis indigotica through analyzing the yield and contents of polysaccharide of Radix Isatis for different treatments.</p><p><b>METHOD</b>An orthogonal experiment design on the basis of three factors and four levels was applied for studying the effect of formula fertilization on yield. The contents of polysaccharides were determined with phenol-witriolic colorimetry.</p><p><b>RESULT</b>The optimum formula fertilization of Radix Isatis was carbamide 869.0 kg x hm(-2), superphosphate 1 428.6 kg x hm(-2) and potassium sulfate 0 kg x hm(-2).</p><p><b>CONCLUSION</b>Superphosphate can observably influence the yields of Radix Inditis. while carbamide influence the contents of polysaccharide of Radix Inditis.</p>

Analysis on biomasses and contents of active components in roots of Salvia miltiorrhiza from different populations / 中国中药杂志

<p><b>OBJECTIVE</b>To provide some evidence for breeding excellent lines of Salvia miltiorrhiza.</p><p><b>METHOD</b>Contents of tanshinone II(A), cryptotanshinone, salvialic acid A and salvianolic acid B in root of S. miltiorrhiza from different populations were determined by HPLC. Total tanshinone and total salvianolic acids were determined by spectrophotometry.</p><p><b>RESULT</b>The biomass of individual plant from Zhongjiang of Sichuan province, content of tanshinone II(A), from Shanxi province, cryptotanshinone and total tanshinone from Zhongjiang of Sichuan province, salvialic acid A of High-Stem cultivation from Hebei province, salvianolic acid B and total salvianolic acids of tetroploid were higher than the other samples, respectively. With the value of biomass of individual plant multiplying contents of every active components as indicative constituents, the multiplying value from Zhongjiang of Sichuan province was higher than the other samples.</p><p><b>CONCLUSION</b>With biomasses and effective components as indicative constituents, it suggested that S. miltiorrhiza from Zhongjiang of Sichuan province was a better derivative material.</p>

Chemical constituents from water extract of Radix isatidis / 药学学报

<p><b>AIM</b>To study the chemical constituents from water extract of Radix isatidis. (Isatis indigotica Fort. ).</p><p><b>METHODS</b>The water extract was underwent absorption by D101 macroporous resin, the portion eluted by ethanol of different concentrations was isolated and purified on silica gel column repeatedly. The obtained compounds were identified and structurally elucidated by their physico-chemical properties and spectral analysis.</p><p><b>RESULTS</b>Five compounds were isolated from water extract of Radix isatidis, and were partly identified separately: 3-[2'-(5'-hydroxymethyl) furyl] -1 (2H) -isoquinolinone-7-O-beta-D-glucoside (I), lariciresinol-4,4'-di-O-beta-D-glucopyranoside (II), lariciresinol-4-O-beta-D-glucopyranoside (III), 2-hydroxy-1, 4-benzenedicarboxylic acid (IV), mannitol (V).</p><p><b>CONCLUSION</b>Compound I is a new compound and compounds IV and V were isolated from the plant for the first time.</p>

Comparison of the botanic morphology and blooming characteristics of four cultivars of rose / 中国中药杂志

<p><b>OBJECTIVE</b>To establish identifying method for further development and utilization by studying botanic morphology and blooming characteristics of four varieties of roses in Jiangsu province.</p><p><b>METHOD</b>Flower-bud and flower-form were observed by dissection and plant modality and blooming process were investigated.</p><p><b>RESULT AND CONCLUSION</b>The flower form and plant modality was obviously different among the 4 varieties of roses. The process of differentiation of flower-bud could be divided into five stages: the transformation of nutritive growth cone, the occurrence and development of sepal, formation of petal primordium, formation of pistil and stamen. The blooming process was made up of flower-bud period, display-petal period, initiating blooming period, blooming period, withering period and corresponding biological marks.</p>