RESUMO
Investigation of ultrastructural peculiarities of morpho-functional changes of macrophages have been studied with the purpose of determining the dynamics and thrust of destructive-necrotic processes in these cells when the ischemic-gangrenous form of diabetic foot syndrome develops show what under the influence of intravenous ozone therapy stimulant effect on functional activity and beneficial effect on elimination, mainly due to genetically programmed cell death (apoptosis), playing a significant role in the regulatory mechanisms of the inflammatory process. The stimulation of macrophages functional activity under the influence of ozone, as well as the presence of destructive changes in such cells without necrotizing lesions, is explained by the inclusion of the mechanism of apoptosis as a positive factor in the regulation of local homeostasis at the completion of the inflammatory (exudative) stage of the wound process.
Assuntos
Complicações do Diabetes/terapia , Macrófagos/ultraestrutura , Ozônio/uso terapêutico , Administração Intravenosa , Apoptose , Estudos de Casos e Controles , Complicações do Diabetes/patologia , Pé Diabético/patologia , Pé Diabético/terapia , Gangrena/patologia , Gangrena/terapia , Humanos , Inflamação/patologia , Inflamação/terapia , Mitocôndrias/ultraestrutura , Necrose , Ozônio/administração & dosagem , Solução Salina/administração & dosagem , Solução Salina/uso terapêuticoRESUMO
The severity of purulent-inflammatory process in patients with diabetes mellitus is determined by lymphocytotoxic test. The test shows that application of intravenous ozone therapy with individually selected ozone dose significantly decreases the spread of necrotic suppurative focus already on the third day of treatment. Granulation tissue and marginal epithelization in the wound develops on the 6-8th day of hospitalization; normalization of glycemic levels shorten of the period of the hospital stay up to 3-5 days, compared to the control group.
Assuntos
Diabetes Mellitus/tratamento farmacológico , Pé Diabético/tratamento farmacológico , Fatores Imunológicos/uso terapêutico , Ozônio/uso terapêutico , Supuração/tratamento farmacológico , Alprostadil/uso terapêutico , Amputação Cirúrgica/métodos , Anti-Inflamatórios/uso terapêutico , Glicemia/efeitos dos fármacos , Glicemia/metabolismo , Diabetes Mellitus/sangue , Diabetes Mellitus/patologia , Diabetes Mellitus/cirurgia , Pé Diabético/sangue , Pé Diabético/patologia , Pé Diabético/cirurgia , Esquema de Medicação , Heparina/uso terapêutico , Humanos , Niacina/uso terapêutico , Pentoxifilina/uso terapêutico , Supuração/sangue , Supuração/patologia , Supuração/cirurgiaRESUMO
Our study evaluated the levels of peroxide oxidation of lipids, oxidative modification of proteins, antioxidant protection and dynamic changes in markers of toxicity in patients with diabetes mellitus and purulent-inflammatory complications. In total, 124 patients were enrolled in the study and were divided into two groups according the treatment methods. Study group consisted of 53 patients, who received intravenously ozonized saline in addition to conservative treatment. The control group consisted of 71 patients who received only conventional therapy. The study period was 6-15 days. The results showed that the use of ozone therapy is not accompanied by negative injury responses at the level of hemostasis parameters and blood biochemical characteristics. Furthermore, ozone therapy may have a favorable effect on treatment outcome in patients with purulent-inflammatory complications and daibetes mellitus.
Assuntos
Abscesso/terapia , Complicações do Diabetes/terapia , Ozônio/uso terapêutico , Infecções dos Tecidos Moles/terapia , Abscesso/sangue , Antioxidantes/metabolismo , Biomarcadores/sangue , Estudos de Casos e Controles , Complicações do Diabetes/sangue , Complicações do Diabetes/cirurgia , Humanos , Peroxidação de Lipídeos , Oxirredução , Carbonilação Proteica , Infecções dos Tecidos Moles/sangue , Infecção da Ferida Cirúrgica/sangue , Infecção da Ferida Cirúrgica/terapiaRESUMO
Studying the effect of ozone therapy on the course of pyoinflammatory processes with diabetes mellitus in the experiment, conducted on 30 white 24-30 month rats, weight 300-450 gr with purulent-inflammatory processes, it was found out that diabetes, which was simulated by subcutaneous injection of alloxan, causes mosaic disturbances of hemostasis system in the presence of pyoinflammatory processes. Complicated changes in blood condition were also detected against the background of diabetes mellitus: chronometric hypocoagulation on the intrinsic pathway of blood coagulation in association with chronometric hypercoagulation by the external thrombinogenesis mechanism and fibrinogenesis depression against the background of hypofibrinogenaemia. Thus, the use of ozone therapy in the presence of soft tissues abscesses in old rats with diabetes does not demonstrate significant protective properties with reduced azoalbumin lysis, total non-enzymatic fibrinolytic activity and proteinase activity by Kunitz in blood plasma. Such changes in old rats can be considered as an exacerbation of purulent inflammation, increase in toxicity, reduced protective properties, increase in manifestations of hypercoagulation against the background of chronic course of diabetes, caused by age-related changes.