Assuntos
Colostro/imunologia , Endopeptidases/farmacologia , Imunoglobulina A Secretora/análise , Imunoglobulina A/análise , Imunoglobulina G/análise , Imunoglobulina M/análise , Suínos/imunologia , Animais , Cromatografia em Gel , Eletroforese em Gel de Poliacrilamida , Feminino , Suco Gástrico , Imunoeletroforese , Testes de Neutralização , Pepsina A/farmacologia , Gravidez , Vírus da Gastroenterite Transmissível/imunologia , Tripsina/farmacologiaRESUMO
To ascertain what class of immunoglobulin (Ig; IgA, IgG, or IgM) is most efficacious in protection, a large quantity of colostrum from sows immunized with virulent transmissible gastroenteritis (TGE) virus was fractionated by chromatographic and gel filtration methods. The isolated IgG, IgA, and IgM(A) had specific virus-neutralizing activities of 1:7.6, 1:342, and 1:302 per milligram of protein, respectively. Each Ig was fed to groups of hysterectomy-derived colostrum-deprived neonatal pigs before and after exposure (challenge) with virulent TGE virus. The 7 pigs fed IgG survived the challenge exposure, but 2 of 7 fed IgA and 1 of 7 fed IgM(A) died of TGE. Three of the survivor pigs that had been fed IgG and 2 of the survivor pigs that had been fed IgA had increased serum antibody titers between 8 and 19 days after challenge exposure, but none of the survivor pigs fed IgM(A) had TGE antibody. In contrast, 12 of 14 virus-control pigs died of TGE and the 2 survivors had antibody conversion. The data show that all 3 Ig classes in immune colostrum will protect neonatal pigs against exposure with virulent TGE virus.
Assuntos
Colostro/imunologia , Gastroenterite Suína Transmissível/prevenção & controle , Imunoglobulina A , Imunoglobulina G , Imunoglobulina M , Animais , Animais Recém-Nascidos , Formação de Anticorpos , Feminino , Gastroenterite Suína Transmissível/imunologia , Imunização Passiva , Gravidez , Suínos , Vacinação/veterináriaRESUMO
Similar immunoglobulin (Ig) classes were obtained from porcine colodtral whey by either column or batch chromatographic procedures; a stepwise buffer elution technique was used. Specific transmissible gastroenteritis virus neutralizing antibody was found in the 4 major fractions eluted comprising of IgG1, IgG2, IgA, and IgM. The IgG1, and IgG2 were essentially homogeneous, and the IgA- AND IgM-rich fractions had to be recycled several times through Sephadex G-200 to obtain pure IgA and IgM that had specific virus neutralizing activities per mg of protein of 342.1 and 302. 4, compared with 7.6 for IgG. By a combination of the batch chromatographic procedures and gel filtration, gram amounts of specific Ig could be fractionated from the same colostrum.