Potassium phosphate and potassium carbonate administration by feed or drinking water improved broiler performance, bone strength, digestive phosphatase activity and phosphorus digestibility under induced heat stress conditions.

Potassium phosphate (K2HPO4) and potassium carbonate (K2CO3) administration by feed or water were evaluated on broiler performance, bone strength, alkaline phosphatase activity (ALP), and phosphorus digestibility under heat stress and high chloride condition. Experimental groups include control; 15 cc/kg K2HPO4; 30 cc/kg K2HPO4; 15 cc/l K2HPO4; and 3.7 g/kg K2CO3. Body weight (BW), feed and water consumption, plasma potassium, phosphorus, and calcium concentration along with plasma and digestive ALP and intestinal digesta pH were measured during the trial. Tibia ash, calcium and phosphorus content, and breaking strength were measured on days 21 and 42 and phosphorus digestibility on day 36 of age. As a result of this, study feed and water consumption was increased by supplementation of the feed or water with K2HPO4 (P ≤ 0.001). K2HPO4 increased body weight at 42 days of age (P ≤ 0.001). Tibia ash and phosphorus content was increased by K2HPO4 supplementation (P ≤ 0.004; P ≤ 0.003). K2CO3 did increased tibia ash but not changed tibia phosphorus content significantly. Tibia shear force, shear energy, extension, and length were improved by K2HPO4 administration at 42 days of age (P ≤ 0.001). Administration of either feed or water with K2HPO4 increased plasma potassium, phosphorus, and calcium concentration at 21 days of age, whereas K2CO3 reduced plasma potassium at 21 days of age (P ≤ 0.05). Plasma ALP reduced by addition of 15 cc K2HPO4 and K2CO3 to diets at 42 days of age, whereas digestive ALP was increased by inclusion of K2HPO4 and not by K2CO3. Supplementation of either feed or water with K2HPO4 increased phosphorus digestibility, whereas K2CO3 reduced phosphorus digestibility (P ≤ 0.003). Jejunum and ileum pH was reduced by K2HPO4 or by K2CO3 at 21 and 42 days of age (P ≤ 0.006; (P ≤ 0.05). Over all, results of current study revealed that K2HPO4 can be a suitable potassium salt choice instead of KCL in hot weather conditions especially when the water or diet contains high levels of chloride.

Effects of gelatin prepared from calf bones rich in phosphorus on broiler performance, bone characteristics and digestive enzymes activity.

1. Gelatin prepared from calf bones (GCB) is a novel source of high-quality protein and phosphorus. Its inclusion in broiler chicken diets may improve bone strength, plasma and digestive alkaline phosphatase activity (ALP), phosphorus digestibility and performance of broilers. Therefore, di-calcium phosphate in a corn-soy control diet was replaced with 12, 24, and 36 g/kg of GCB in a completely randomised design with four treatments of six replicates and 10 chicks in each pen. The trial lasted from 1 to 42 d of age. 2. Body weight and feed intake were measured weekly. Plasma calcium and phosphorus concentration along with plasma and digestive ALP were assayed throughout the trial. Trypsin, α-amylase, lipase and total protease activity were assayed at 14 and 28 d of age. Tibia ash, calcium and phosphorus content and breaking strength were measured at 14, 28 and 42 d of age. Phosphorus digestibility was measured at 36 d of age. 3. Body weight and feed intake showed no significant differences between controls and diets containing 12 and 36 g/kg GCB. Tibia ash and tibia length were increased by supplementation of GCB (P ≤ 0.001). Tibia calcium and phosphorus content were increased by GCB inclusion at 14 d of age (P ≤ 0.001). Digestive alkaline phosphatase activity was increased and trypsin activity was reduced by inclusion of GCB (P ≤ 0.001; P ≤ 0.004). α-amylase activity decreased by inclusion of 12 and 24 g/kg GCB, whereas an increase in α-amylase activity was observed by inclusion of 36 g/kg GCB (P ≤ 0.001). Supplementation of diets with GCB increased phosphorus digestibility (P ≤ 0.01) and suppressed ileum growth during the experimental period. 4. Results of the current study showed that phosphorus from gelatin can greatly improve broiler bone characteristics and phosphorus digestibility and complete elimination of inorganic phosphate sources from broiler diets is feasible with inclusion of 36 g/kg high phosphorus gelatin.

Arginine, soy isoflavone and hydroxypropylmethylcellulose have protective effects against obesity in broiler breeder hens fed on high-energy diets.

1. The present study was undertaken to determine the effects of arginine, soy isoflavone (ISF) and hydroxypropylmethylcellulose (HPMC) on obesity in broiler breeder hens. 2. A total of 320 Cobb 500 hens, 45 weeks of age, were assigned to 64 floor pens. The experiment was conducted as a completely randomised design in a factorial arrangement (2 × 2 × 2 × 2) with 4 replicates of 5 hens in each pen. Factors included two concentrations of HPMC (0 and 1%), two concentrations of arginine (8.4 and 12 g/kg), two concentrations of ISF (zero and three times more than that present in basal diets) and two contents of energy (11.7 and 14.6 MJ/kg). Performance criteria and blood characteristics of hens were measured during the experimental period. Expression of genes involved in lipid metabolism was determined in the liver at 55 weeks of age. 3. Hens given high-energy diets showed increased BW (body weight), ovary weight and abdominal fat pad and enhanced plasma glucose, triglyceride (TG), cholesterol, haemoglobin, haematocrit and low lymphocyte percentages. The expression of malic enzyme, peroxisome proliferator-activated receptor-α (PPARα), peroxisome proliferator-activated receptor-γ (PPARγ) and inducible nitric oxide (iNOS) increased and sterol regulatory element binding protein-1c (SREBP1c) decreased with increasing energy content of diets. Arginine addition decreased TG, cholesterol and A1-c haemoglobin concentration and increased PPARα, PPARγ and iNOS expression. Inclusion of ISF and HPMC decreased BW, egg weight, plasma TG, cholesterol and increased egg production and also enhanced PPARγ and iNOS expression. Significant interactions were observed between energy concentration and ISF and HPMC on BW. 4. The results of the current study revealed that ISF, HPMC and arginine have beneficial effects on controlling the metabolism of obese broiler breeder hens and using a mix of these products minimises the harmful effects of obesity.

Black cumin seeds, Artemisia leaves (Artemisia sieberi), and Camellia L. plant extract as phytogenic products in broiler diets and their effects on performance, blood constituents, immunity, and cecal microbial population.

Two experiments were conducted to evaluate the effects of using black cumin seeds (BCS), Artemisia leaves (AL), and Camellia L. plant extract (CLE) in the diets of broiler chicks. Experiment 1 was conducted as a completely randomized design in a factorial arrangement (2 × 2) with 8 replicates of 4 chicks in each battery cage. Factors included 2 levels of BCS and AL (0 and 1%). Experiment 2 was conducted as a completely randomized design with 4 treatments (control, 0.3 and 0.5 g/kg of CLE, and 0.5 g/kg of mannanoligosaccharide) of 8 replicates and 4 chicks in each. Body weight and cumulative feed intake were measured at 21, 35, and 42 d of age. Antibody response against SRBC was measured on d 28 and 42. Blood characteristics, relative weight and length of different parts of the carcass, gastrointestinal pH, villi length, and crypt depth were measured at 42 d of age. Artemisia addition did not affect BW and feed conversion ratio (FCR) but decreased feed intake significantly up to 21 d of age (P ≤ 0.01). Black cumin significantly increased BW (P ≤ 0.05) at 21 and 42 d of age and decreased FCR throughout the experimental period (P ≤ 0.01). Artemisia significantly increased monocytes but had no effect on gastrointestinal pH, antibody response, and relative weight and length of different parts of the carcass. Black cumin increased red blood cells, hematocrit, hemoglobin, gizzard relative weight, and pH but decreased antibody response and monocytes percentage (P ≤ 0.01). Artemisia did not affect plasma lipid profile but decreased coliform and Escherichia coli populations of ceca significantly (P ≤ 0.01 and P ≤ 0.05, respectively). Addition of 0.5 g/kg of CLE decreased BW, feed intake, and FCR throughout the experiment (P ≤ 0.01). Camellia increased gizzard and proventriculus pH, villi length, and crypt depth (P ≤ 0.01) but decreased primary antibody response, total white blood cell count, and cholesterol concentration (P ≤ 0.05). The results of this experiment showed that using BCS alone or mixed with AL improved broiler health and performance but CLE negatively affected broiler BW and feed intake and is not a good alternative to commercial mannanoligosaccharide.