Hepatoprotective Angelica sinensis silver nanoformulation against multidrug resistant bacteria and the integration of a multicomponent logic gate system.

The rampant usage of antibiotics has led to the emergence of toxicity, especially hepatotoxicity and the emergence of microbial drug resistance. Hence, a series of novel hepatoprotective, biocompatible, antibacterial silver nanoformulations (AS-AgNPs) were developed by using the important Chinese medicinal plant Angelica sinensis. The different size of AS-AgNPs were characterized by UV-Visible spectroscopy, transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR). The size-dependent antibacterial properties of AS-AgNPs were investigated against Gram-positive, Gram-negative and multi-drug resistant bacteria. The minimum inhibitory concentration (MIC) of AS-AgNPs with different size against six bacteria was found to be in the range of 5-100 µg mL-1 with no resistance till 12 cycles. TEM and SEM results of bacteria after the treatment suggested that AS-AgNPs disrupted the cell membrane by creating pores. The cytocompatibility and cytoprotective effect of AS-AgNPs were evaluated against HepG2 cell lines, which showed that 85% of cells were viable up to 100 µg mL-1 of the concentration with almost no change in AST and ALT levels. Further, a logic combinatorial library, including basic logic gates (AND, OR, NOR, INHIBIT, IMPLICATION, and YES), three input logic gates (OR, and NOR) and combinatorial gates (INH-OR, INH-YES, INH-INH, AND-NOR, and NOT-AND-NOR) were designed by integrating multi-components based on the interaction between AS-AgNP1 and bacteria, where DiSC3(5) was used as the signal reporter. This system clearly demonstrates the ability of simple logic circuits to perform sophisticated analysis for the detection of multiple bacteria.

Nanoclay-based drug delivery systems and their therapeutic potentials.

Safe, therapeutically effective, and patient-compliant drug delivery systems are needed to design novel tools and strategies to combat the deadliest of diseases such as cancer, SARS, H7N9 avian influenza, and dengue infection. The major challenges in drug delivery are cytotoxicity, poor biodistribution, insufficient functionality, ineffective drug incorporation in delivery devices, and subsequent drug release. Clay minerals are a class of nanolayered silicates that have good biocompatibility, high specific surface area, chemical inertness, colloid, and thixotropy, and are attractive practical and potential nanomaterials in medicine. These properties enable the usage of nanoclays as drug carriers for the delivery of antibiotics, antihypertensive drugs, anti-psychotic, and anticancer drugs. The review examines the latest advances in nanoclay-based drug delivery systems and related applications in gene therapy and tissue engineering. Clay minerals, particularly montmorillonite, kaolinite, and halloysite are used to delay and/or target drug release or even improve drug dissolution due to their surface charge. Chemical modification of clay minerals such as intercalation of ions into the interlayer space of clay minerals or surface modification of clay minerals is a strategy to tune the properties of nanoclays for the loading and release of a drug. The modified nanoclay can take up drugs by encapsulation, immobilization, ion exchange reaction, or electrostatic interactions. Controlled drug release from the drug-clay originates from the incorporation and interactions between the drug and inorganic layers, including electrostatic interactions and hydrogen bonding. Montmorillonite has proven non-toxic through hematological, biochemical, and histopathological analyses in rat. Montmorillonite can also act as a potent detoxifier. Halloysite nanotubes can bind synthetic and biological components such as chitosan, gelatin, and alginate innate nanocarriers for the improved loading and controlled release of drugs, proteins, and DNA. The peculiar properties of clay nanoparticles lead to promising applications in drug delivery, gene delivery, tissue engineering, cancer and stem cell isolation, and bioimaging.

Removal of toxic contaminants from water by sustainable green synthesised non-toxic silver nanoparticles.

The study describes the synthesis of silver nanoparticles using 21 different plant extracts having medicinal properties. Molecular ultraviolet-visible spectroscopy shows that the λmax of nanoparticles synthesised by different plant extracts varied and ranged between 400 and 468 nm. The ultraviolet results revealed that although synthesis of nanoparticles occurred by all plant extracts successfully, their size varies, this was further confirmed by differential light scattering. The synthesised nanoparticles were investigated for their antimicrobial properties. The most promising silver nanoparticles Ocimum sanctum and Artemisia annua assisted were further characterised using transmission electron microscopy and energy dispersive X-ray spectroscopy (EDX). EDX data confirms that synthesised nanoparticles are highly pure. Further these two plant assisted nanoparticles were studied for chemocatalytic and adsorptive properties. The silver nanoparticles from Ocimum sanctum can catalyse the reduction of 4-nitrophenol (63%) within 20 min in the presence of NaBH4, whereas Artemisia annua assisted silver nanoparticles did not show significant chemocatalytic activity. Both the promising nanoparticles can efficiently adsorb textile dyes from aqueous solutions. These synthesised nanoparticles were also exploited to remove microbial and other contaminants from Yamuna River water. The nanoparticles show excellent antimicrobial properties and can be reused repeatedly.