1.
J Chromatogr B Analyt Technol Biomed Life Sci
; 927: 218-22, 2013 May 15.
Artigo
em Inglês
| MEDLINE
| ID: mdl-23394897
RESUMO
An ultra high pressure liquid chromatographic method coupled with diode array detector (UHPLC-DAD) has been developed and validated for the fast separation and determination of three major methylxanthines, i.e., caffeine, theophylline and theobromine, in various herbal beverages. Isocratic elution using 0.1vol% formic acid/CH3OH (92.5:7.5, v/v) enabled the completion of the separation cycle in less than 3min using a flow rate of 0.7mL/min and a column temperature of 50°C. Validation of the method included linearity (0.5-50mg/L), limits of detection (12-35µg/L) and quantification (40-120µg/L), precision, matrix effect and accuracy. The percent recoveries ranged between 90 and 108%.